L-theanine zinc chelate and preparation method thereof, as well as tea drink containing L-theanine zinc chelate, and preparation method thereof

A technology of zinc theanine and chelates, which is applied in the preparation of organic compounds, carboxylic acid amide preparation, chemical instruments and methods, etc., can solve the problem of expensive and time-consuming electrolytic synthesis, and the lack of L-theanine and zinc ions. Related reports and other issues to achieve the effect of improving the body's immune system, increasing bioavailability, and fast absorption

Inactive Publication Date: 2017-07-07
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Electrosynthesis is expensive and time-consuming
At present, there are more researches at home and abroad on zinc glycine, zinc methionine, zinc glutamate, etc., but there are almost no relevant reports on the chelate of L-theanine and zinc ions.

Method used

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  • L-theanine zinc chelate and preparation method thereof, as well as tea drink containing L-theanine zinc chelate, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for preparing a tea beverage containing L-theanine zinc chelate, comprising the steps of:

[0035] First: Inoculate Bacillus subtilis stored on a slant at 4°C on a plate medium, culture at 37°C for 16 hours, and then transfer to 3mL liquid medium for activation. The secondary seed liquid of Bacillus subtilis activated twice was transferred to 30 mL of fermentation culture liquid according to the inoculation amount of 3% volume fraction, placed in a constant temperature incubator for shaking culture at 37°C, and cultured overnight for 12 hours.

[0036] Second: collect the fermented liquid and centrifuge at 8000 rpm for 25 minutes to obtain crude enzyme liquid of γ-glutamyl transpeptidase. Enzyme activity was measured using a spectrophotometer. Select 20 mL of crude enzyme solution with an enzyme activity of 0.01 U / mL for the catalytic reaction.

[0037] The 3rd: add respectively the L-glutamine (concentration 15mmol / L) of crude enzyme liquid weight 10% and et...

Embodiment 2

[0045] A method for preparing a tea beverage containing L-theanine-zinc chelate, comprising the steps of:

[0046] First: Inoculate Bacillus subtilis stored on a slant at 4°C on a plate medium, culture at 37°C for 16 hours, and then transfer to 3mL liquid medium for activation. The secondary seed liquid of Bacillus subtilis activated twice was transferred to 30 mL of fermentation culture liquid according to the inoculum amount of 3% volume fraction, placed in a constant temperature incubator for shaking culture at 37°C, and cultured overnight for 14 hours.

[0047] Second: collect the fermented liquid and centrifuge at 8000 rpm for 25 minutes to obtain crude enzyme liquid of γ-glutamyl transpeptidase. Enzyme activity was measured using a spectrophotometer. Select 20 mL of crude enzyme solution with an enzyme activity of 0.15 U / mL for the catalytic reaction.

[0048] The 3rd: add respectively the L-glutamine (concentration 30mmol / L) of crude enzyme liquid weight 10% and ethyl...

Embodiment 3

[0056] A method for preparing a tea beverage containing L-theanine-zinc chelate, comprising the steps of:

[0057] First: Inoculate Bacillus subtilis stored on a slant at 4°C on a plate medium, culture at 37°C for 16 hours, and then transfer to 3mL liquid medium for activation. The secondary seed liquid of Bacillus subtilis activated twice was transferred to 30 mL of fermentation culture liquid according to the inoculation amount of 3% volume fraction, and placed in a constant temperature incubator for shaking culture at 37°C, and cultured overnight for 16 hours.

[0058] Second: collect the fermented liquid and centrifuge at 8000 rpm for 25 minutes to obtain crude enzyme liquid of γ-glutamyl transpeptidase. Enzyme activity was measured using a spectrophotometer. Select 20 mL of crude enzyme solution with an enzyme activity of 0.2 U / mL for the catalytic reaction.

[0059] The 3rd: add respectively the L-glutamine (concentration 50mmol / L) of crude enzyme liquid weight 10% and...

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Abstract

The invention discloses an L-theanine zinc chelate and a preparation method thereof, as well as a tea drink containing the L-theanine zinc chelate, and a preparation method thereof. The preparation method of the L-theanine zinc chelate comprises the following steps: building a reaction system, wherein L-theanine and zinc salt are used as raw materials, and a buffer solution with pH of 2-7 is used as a reaction medium; mixing at 30-70 DEG C; and performing separation and purification on a reaction solution after complete reaction to obtain the L-theanine zinc chelate. The mass ratio of the L-theanine to the zinc salt is (5-1):1; the L-theanine is prepared by fermenting gamma-glutamyltranspeptidase produced by fermentation of bacillus subtilis by taking L-glutamine and ethylamine hydrochloride as substrates. The L-theanine is prepared by using a microbial enzyme method; on the base, zinc ions and the L-theanine are chelated by using a water system synthesis method to obtain the L-theanine zinc chelate; the production cost is low, the environmental safety is high, the resource consumption is small, and the large-scale safety production is achieved.

Description

technical field [0001] The invention relates to a chelate compound, a functional tea drink containing it and a preparation method, in particular to a L-theanine zinc chelate compound, a preparation method, a tea drink containing the chelate compound, and a preparation method. Background technique [0002] In recent years, the research on the relationship between the trace element zinc and human health has been paid more and more attention, and related zinc supplement products have also been enriched. Zinc supplementation products currently on the market can be divided into three generations: the first generation is inorganic form of zinc, representing zinc sulfate, zinc acetate, etc.; the second generation is organic form of zinc, representing zinc gluconate, zinc glycyrrhizinate, zinc lactate and Zinc glutamate, etc.; the third generation is zinc in biological form, representing protein zinc, etc. The first-generation zinc is still partly sold in clinical medicine, but it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C231/12C07C237/06A23L33/165A23L33/175A23F3/34C12P13/04
CPCC07C231/12A23F3/34A23V2002/00C07C237/06C12P13/04A23V2200/324A23V2250/0646A23V2250/1642
Inventor 陈立李建科
Owner SHAANXI NORMAL UNIV
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