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Preparation method and application of a compound inhibitor of honeysuckle polyphenol oxidase

A polyphenol oxidase and compound inhibitor technology, applied in the field of traditional Chinese medicine processing, can solve the problems of insignificant increase of luteolin content and large equipment investment, and achieve the effect of accurate measurement and content improvement.

Inactive Publication Date: 2019-10-29
HEBEI UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Drying methods such as vacuum drying, microwave vacuum combined drying, and heat pump far-infrared combined drying have problems such as large investment in equipment, and ineffective improvement of the content of the index component luteolin in honeysuckle.

Method used

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  • Preparation method and application of a compound inhibitor of honeysuckle polyphenol oxidase
  • Preparation method and application of a compound inhibitor of honeysuckle polyphenol oxidase
  • Preparation method and application of a compound inhibitor of honeysuckle polyphenol oxidase

Examples

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Effect test

preparation example Construction

[0031] ④ Preparation of reference solution

[0032] Accurately weigh 1.06mg luteolin reference substance and 10.02mg chlorogenic acid reference substance, put them in a 25mL brown measuring bottle, add 70% ethanol to dissolve and constant volume, and prepare luteolin containing 42.40μg per 1mL, containing 400.80μg The mixed reference substance solution of chlorogenic acid is stored in refrigerator;

[0033] ⑤ Preparation of the test solution

[0034] Accurately weigh about 2 g of honeysuckle powder (passed through a No. 4 sieve), put it in a 200 mL conical flask with a stopper, accurately add 50 mL of 70% ethanol, weigh it, ultrasonically (power 250 W, frequency 35 kHz) for 60 min, let it cool, and weigh again Weight, use 70% ethanol to make up for the loss, shake well, filter, accurately measure 10mL of the filtrate, evaporate the solvent, add 70% ethanol to the residue, put it in a 5mL brown measuring bottle, add 70% ethanol to volume, shake Evenly, to obtain final product...

Embodiment 1

[0042] 1. Inhibition of browning of fresh honeysuckle

[0043] (1) Selection of raw material honeysuckle: select fresh, no disease, no obvious damage to the epidermis, no yellowing fresh honeysuckle flower buds.

[0044] In this example, honeysuckle was collected from Julu (the third batch of honeysuckle) in Xingtai City, Hebei Province on July 28, 2016.

[0045] (2) Configuration of compound polyphenol oxidase inhibitor formula one: vitamin C, β-cyclodextrin, kojic acid, and sodium chloride were weighed according to mass percentages of 0.01%, 0.10%, 0.40%, and 0.50%, respectively, and Dissolve in 100mL water.

[0046] (3) Anti-browning treatment: Soak the selected honeysuckle in the prepared compound polyphenol oxidase inhibitor aqueous solution at a solid-to-liquid ratio of 1:8 (mass ratio) for 120 minutes, then take it out and drain.

[0047] (4) Drying treatment. The honeysuckle samples treated with the compound polyphenol oxidase inhibitor formula 1 and the untreated hone...

Embodiment 2

[0050] 1. Inhibition of browning of fresh honeysuckle

[0051] (1) Selection of raw material honeysuckle: select fresh, no disease, no obvious damage to the epidermis, no yellowing fresh honeysuckle flower buds.

[0052] In this example, honeysuckle was collected from Julu (the third batch of honeysuckle) in Xingtai City, Hebei Province on July 28, 2016.

[0053] (2) Configuration of compound polyphenol oxidase inhibitor formula two: vitamin C, β-cyclodextrin, kojic acid, and sodium chloride were weighed according to mass percentages of 0.015%, 0.10%, 0.40%, and 1.00%, respectively, and Dissolve in 100mL water.

[0054] (3) Anti-browning treatment: Soak the selected honeysuckle in the prepared compound polyphenol oxidase inhibitor aqueous solution at a solid-to-liquid ratio of 1:8 (mass ratio) for 120 minutes, then take it out and drain.

[0055] (4) Drying treatment, the honeysuckle samples treated with the compound polyphenol oxidase inhibitor formula 2 and the untreated h...

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Abstract

The invention provides a honeysuckle flower and polyphenol oxidase composite inhibitor, a preparation method and application, and belongs to the field of processing of traditional Chinese medicines. The honeysuckle flower and polyphenol oxidase composite inhibitor is an aqueous solution, takes water as a solvent and comprises the following ingredients by mass percentage: 0.1-0.4% of kojic acid, 0.005-0.015% of vitamin C, 0.05-0.15% of beta-cyclodextrin and 0.5-1.5% of sodium chloride. Through determination with an HPLC (high performance liquid chromatography), the content of galuteolin in honeysuckle flowers is obviously improved. According to the formula provided by the invention, the problems of browning and decrease of the content of effective ingredients of the honeysuckle flowers caused by polyphenol oxidase can be solved, the effect is good, the operation is simple and convenient, and the price is low.

Description

technical field [0001] The invention relates to a preparation method and application of a compound inhibitor of honeysuckle polyphenol oxidase, and belongs to the field of traditional Chinese medicine processing. Background technique [0002] Honeysuckle is the dry flower bud or the first flower of Lonicera Lonicerae, which is cold in nature and sweet in taste; it returns to the lung and stomach meridians; it has the effects of clearing away heat and detoxifying, and evacuating wind-heat. As a large amount of traditional Chinese medicine for both medicine and food, honeysuckle has a high health care function and a very high economic value. The honeysuckle picking period is from May to September every year. Fresh honeysuckle is green and white in color, full of flower buds, high in water content, and difficult to store. The main preservation method of honeysuckle is dehydration and drying immediately after picking. At present, the drying method of honeysuckle in my country m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 李菁马东来郑玉光冯建明
Owner HEBEI UNIV OF CHINESE MEDICINE