A kind of composite biological preparation and its application

A technology for biological preparations and crop nutrition, applied in the direction of biocides, chemicals for biological control, biocides, etc., can solve the problem that the density of mycelium is not as good as that of fermentation alone, the growth and reproduction behaviors interfere with each other, and the effect of plant disease control is affected. It can improve soil stability and porosity, reduce labor input, and improve the effect of prevention and control.

Active Publication Date: 2018-10-02
莱阳市圣德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If mixed fermentation is used, the growth and reproduction behaviors of the two fungi will interfere with each other, resulting in mycelium density not as good as the sum of separate fermentations, and the amount of spore production is also difficult to satisfy
This will greatly affect the control effect of plant diseases

Method used

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  • A kind of composite biological preparation and its application
  • A kind of composite biological preparation and its application
  • A kind of composite biological preparation and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] The spore suspension of Rhizopus stoloniferus is prepared according to the following method: first, the bacterial classification of refrigerated preservation is inoculated on flat PDA medium with inoculating loop, and described PDA medium is: potato 200g, sucrose 20g, agar 18g, distilled water 1000mL, Transfer to 3 generations; then use the inoculation loop to pick the edge hyphae and transfer to the PDB medium, the PDB medium is: 200g of potatoes, 20g of sucrose, 1000mL of distilled water, the culture temperature is 28°C, and the culture time is 10 days. The fermented liquid is filtered to obtain the spore suspension of Rhizopus stoloniferum, and the live spore concentration is (5~9)×10 8 pieces / ml.

[0054] The spore suspension of described Trichoderma viride is prepared according to the following method: first, the strains preserved in cold storage are inoculated on flat PDA medium with an inoculation loop, and the PDA medium is: potato 200g, sucrose 20g, agar 18g, d...

Embodiment 2

[0060] The spore suspension of Rhizopus stolonifer and the spore suspension of Trichoderma viride were simultaneously inoculated into a fermenter in a weight ratio of 1:1 for submerged fermentation; the rest of the steps and process parameters were the same as in Example 1. Finally, the composite biological preparation of Example 2 of the present invention was obtained.

[0061] Measure its bacteriostatic rate and relative inhibitory rate (%) to Alternaria apple according to the same method as Example 1. See results figure 2 and Table 1.

Embodiment 3

[0063] The spore suspension of Rhizopus stolonifer and the spore suspension of Trichoderma viride were simultaneously inoculated into a fermenter in a weight ratio of 1:9 for liquid submerged fermentation; the rest of the steps and process parameters were the same as in Example 1. The composite biological preparation of Example 3 of the present invention was finally obtained.

[0064] Measure its bacteriostatic rate and relative inhibitory rate (%) to Alternaria apple according to the same method as Example 1. See results figure 2 and Table 1.

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Abstract

The invention discloses a composite biological preparation for preventing and treating soil-borne diseases, which comprises a mixed fermentation broth obtained by submerged fermentation of rhizopus stolonifer and trichoderma viride. Furthermore, its use for controlling soil-borne diseases, preferably Fusarium wilt and Verticillium wilt, is disclosed. The invention utilizes the liquid submerged fermentation technology, and the two kinds of microorganisms reproduce very quickly and have extremely high extracellular secretion activity; the technical effect that cannot be achieved by simple compounding can be realized, and a large amount of bacteria and spores can be obtained rapidly at the same time. The composite biological preparation of the invention has strong bactericidal activity on Alternaria fungi, so it can effectively prevent and control soil-borne diseases such as Fusarium wilt or Verticillium wilt. When used with fertilizer components, it also significantly increases soil stability and porosity.

Description

technical field [0001] The invention belongs to the field of biological technology and relates to a composite biological preparation and its application, in particular to a composite biological preparation for preventing and treating soil-borne diseases and its application. Background technique [0002] Soil-borne diseases of plants refer to diseases in which pathogenic bacteria living in the soil infect plants from the roots or stems of plants under suitable conditions, resulting in wilting and death of plants. Common soil-borne diseases mainly include the following types: Common soil-borne diseases at the seedling stage include damping-off disease caused by Pythium, blight caused by Rhizoctonia solani, etc.; common prominent diseases at the adult plant stage include those caused by Fusarium wilt of melons caused by Fusarium, Verticillium wilt of eggplant caused by Verticillium, bacterial soft rot of Chinese cabbage caused by Erwinia, Pythium root rot caused by Pythium melo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P1/02A01N63/04A01P3/00C05G3/00C05G3/04C12R1/845C12R1/885C05G3/80
CPCC12N1/14C12P1/02C05F11/08C05G3/00A01N63/30C05G3/80C12R2001/845C12N1/145C12R2001/885A01N2300/00
Inventor 杨文慧陈靠山赵明松辛奕
Owner 莱阳市圣德生物科技有限公司
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