Preparation method for titanium dioxide nano particle fluorescence probe for detection DDT

A nanoparticle and titanium dioxide technology, which is applied in the field of material science, can solve the problems of DDT trace detection, etc., and achieve the effect of improving selective recognition, enhancing fluorescence intensity, and improving high-sensitivity detection

Active Publication Date: 2017-07-21
HEFEI UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

At present, there is no report on the modification of amino groups on the surface of the titanium dioxide shell to detect trace amounts of DDT, and...

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  • Preparation method for titanium dioxide nano particle fluorescence probe for detection DDT
  • Preparation method for titanium dioxide nano particle fluorescence probe for detection DDT
  • Preparation method for titanium dioxide nano particle fluorescence probe for detection DDT

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preparation example Construction

[0050] A method for preparing a titanium dioxide nanoparticle fluorescent probe for DDT detection, characterized in that: the aminopropyl group on the imprinted recognition site inside the titanium dioxide nanoparticle fluorescent probe can interact with the DDT molecule entering the recognition site Function, the hydrogen atom on the aminopropyl group forms a hydrogen bond with the chlorine atom on the DDT molecule to stabilize the aminopropyl group. When light is excited, it prevents the electron transfer of the nitrogen atom on the aminopropyl group to the NBD-APTS complex. According to the light-induced The mechanism of electron transfer leads to the enhancement of the fluorescence intensity of the complex. Through the matching of imprinted sites and the change of fluorescence intensity, the recognition and detection of DDT molecules are realized. The preparation process includes the following three steps:

[0051] 1.1 Preparation of NBD-APTS complex: First, accurately weig...

Embodiment

[0056] Example: Firstly, the NBD-APTS complex is prepared according to the covalent coupling reaction, and then the fluorescent probe of titanium dioxide nanoparticles whose surface is rich in amino groups can be obtained according to the non-covalent bond (hydrogen bond) reaction and cross-linking reaction.

[0057]The first step is the preparation of the NBD-APTS complex: First, accurately weigh 0.0016g of 4-chloro-7-nitrobenzofurazan (NBD-Cl) with an electronic balance and place it in a 50mL single Then, use a microsampler with a volume range of 100 μL ~ 1000 μL to draw 220 μL of 3-aminopropyltriethoxysilane (3-aminopropyltriethoxysilane, APTS) into the above flask, and finally, add Add 16 mL of ethanol to the flask, ultrasonically disperse the mixture in the flask for 5 min, and react at 50°C for 2 h at a stirring speed of 500 rpm in an inert atmosphere;

[0058] The second step is the preparation of the EDA-ICPTS complex: First, accurately weigh 0.4240g 4,4'-ethylenebisph...

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Abstract

The invention discloses a preparation method for a titanium dioxide nano particle fluorescence probe for detection DDT. In the method, TiO2 is used as a core, and modified with 4-chloro-7-nitrobenzofuran fluorescence probe and aminopropyl functional groups on the surface, and at last quasi target molecules are printed on the surface, so that a silicon dioxide printed shell layer is formed. The preparation process of the fluorescence probe comprises three steps of 1, preparation of a fluorescence precursor; 2, silanization of the quasi target molecules, and 3, preparation and elution of the titanium dioxide nanoparticles printed with the quasi target molecules. The target molecule DDT enters a recognition site, a helium atom of the DDT and a hydrogen atom of aminopropyl form a hydrogen bond, so that the aminopropyl is stable, when in optical excitation, electrons of the nitrogen atom on the aminopropyl is prevented from being transferred to an NBD-APTS compound, fluorescence strength of the compound is enhanced according to a photoinduced electron transfer mechanism, recognition and detection of the DDT molecule are achieved by matching print sites and changing the fluorescence strength, photocatalysis of TiO2 is excited by changing an excitation wavelength, and thus degradation of the DDT molecule is achieved.

Description

technical field [0001] The invention relates to the field of material science, in particular to a method for preparing a titanium dioxide nanoparticle fluorescent probe used for DDT detection. Background technique [0002] In terms of safety, the use of pesticides has brought us great benefits, but it also poses a serious threat to our country's ecological environment and human health. In particular, pesticide residues in agricultural and animal husbandry products and food have chronic toxicity, direct poisoning, "three causes" (carcinogenic, teratogenic, mutagenic) effects and environmental hormone effects on the human body. Pesticide residues pose a long-term and serious threat to my country's food safety. The public's sense of safety for agricultural products and food has declined significantly, and it has also seriously affected the competitiveness of my country's agricultural products in the international market. Therefore, in recent years, the detection of trace pesti...

Claims

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Application Information

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IPC IPC(8): C09K11/02C09K11/06A62D3/176G01N21/64B01J21/06
CPCA62D3/176A62D2101/04A62D2101/22B01J21/063B01J35/004C09K11/025C09K11/06C09K2211/1088G01N21/643
Inventor 周杨群高大明漆天瑶席小倩代星辰朱德春陈红孙虹张凌云
Owner HEFEI UNIV
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