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Screening medium suitable for extensively drug-resistant Pseudomonas aeruginosa and preparation method

A Pseudomonas aeruginosa and culture medium technology, applied in the field of microbiology, achieves the effects of simple preparation method, high sensitivity and shortened detection time

Active Publication Date: 2021-04-27
廊坊恒益生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of screening culture medium and preparation method applicable to extensively drug-resistant Pseudomonas aeruginosa, thereby solving the aforementioned problems existing in the prior art

Method used

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  • Screening medium suitable for extensively drug-resistant Pseudomonas aeruginosa and preparation method
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  • Screening medium suitable for extensively drug-resistant Pseudomonas aeruginosa and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: the preparation of the nutrient substance that promotes the growth of Pseudomonas aeruginosa

[0037] 1. Pre-treatment of animal blood: purchase 1000ml of pig blood at the farmer’s market, take 100ml and add 900ml of distilled water, use SKG 1246 wall-breaking cooking machine to stir and break the clot to obtain blood mixture, the speed is 1200 rpm, and the time is 3 minute.

[0038] 2. Adjust the pH value: use 1mol / L sodium hydroxide (pH: 14) to adjust the pH value of the blood mixture to 8.5 to obtain the blood raw material solution;

[0039] 3. High-temperature and high-pressure hydrolysis: Put the blood raw material liquid into a high-temperature and high-pressure reaction kettle, and carry out high-temperature and high-pressure hydrolysis reaction while stirring under the conditions of temperature 195°C, atmospheric pressure 2.1 atmospheres, and stirring speed of 100 rpm. For 1h, the hydrolyzate was obtained, and the high-temperature and high-pressur...

Embodiment 2

[0042] Example 2: Comparative test of NAC agar plate and NAC agar plate (without antibiotic combination) added with nutrients that promote the growth of Pseudomonas aeruginosa

[0043] 1. Prepare NAC agar plate

[0044]Weigh 20 g of peptone, 0.3 g of dipotassium hydrogen phosphate, 0.2 g of magnesium sulfate, 0.2 g of cetrimethylene bromide, 0.015 g of nalidixic acid, and 15 g of agar, and dissolve them in 1 L of distilled water, mix well, and pressurize at 121°C After being sterilized for 25 min, it was poured onto a sterile plate and cooled at room temperature to obtain a NAC agar plate, which was set as the control group.

[0045] 2. Prepare NAC agar plates with nutrients that promote the growth of Pseudomonas aeruginosa (no antibiotic combination)

[0046] Weigh 20 g of peptone, 0.3 g of dipotassium hydrogen phosphate, 0.2 g of magnesium sulfate, 0.2 g of cetrimethylene bromide, 0.015 g of nalidixic acid, and 15 g of agar, add 50 ml of filtrate and 950 ml of distilled wat...

Embodiment 3

[0051] Example 3: NAC agar plate and the screening method based on the screening medium suitable for extensively drug-resistant Pseudomonas aeruginosa and its screening medium comparison test

[0052] 1. Prepare NAC agar plate

[0053] Weigh 20 g of peptone, 0.3 g of dipotassium hydrogen phosphate, 0.2 g of magnesium sulfate, 0.2 g of cetrimethylene bromide, 0.015 g of nalidixic acid, and 15 g of agar, and dissolve them in 1 L of distilled water, mix well, and pressurize at 121°C After being sterilized for 25 min, it was poured onto a sterile plate and cooled at room temperature to obtain a NAC agar plate, which was set as the control group.

[0054] 2. Preparation of agar plate for selective isolation and cultivation of extensively drug-resistant Pseudomonas aeruginosa (add antibiotic combination)

[0055] (1) Weigh 20 g of peptone, 0.3 g of dipotassium hydrogen phosphate, 0.2 g of magnesium sulfate, 0.2 g of cetrimethylene ammonium bromide, 0.015 g of nalidixic acid, and 15...

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Abstract

The invention discloses a screening medium suitable for extensively drug-resistant Pseudomonas aeruginosa and a preparation method thereof, and relates to the technical field of microbes. The screening medium includes basic medium, nutrients for promoting the growth of Pseudomonas aeruginosa, antibiotics for detecting multidrug-resistant Pseudomonas aeruginosa, and distilled water. The method: preparing nutrients that promote the growth of Pseudomonas aeruginosa, adding the nutrients that promote the growth of Pseudomonas aeruginosa to the basic medium, and autoclaving to obtain an initial medium; after sterilization, When the temperature of the initial culture medium is between room temperature and 60°C, add antibiotics for the detection of multidrug-resistant Pseudomonas aeruginosa, mix well, dispense into different wells of the sample plate, and cool at room temperature to obtain the antibiotics suitable for extensively drug-resistant Pseudomonas aeruginosa. Screening media for monocysts. The screening medium of the invention has the advantages of shortened detection time, high sensitivity and simple preparation method.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a screening medium suitable for extensively drug-resistant Pseudomonas aeruginosa and a preparation method. Background technique [0002] Pseudomonas aeruginosa is a common opportunistic pathogen that can cause purulent lesions. After infection, the pus and exudate are green, so it is also known as Pseudomonas aeruginosa. It is one of the clinically important opportunistic pathogens. one. [0003] In clinical sample testing, highly suspicious colonies are often selected from the numerous colonies obtained from the test samples by using blood agar culture medium for isolation and culture, and after confirming that the cultured colonies are free from contamination by foreign bacteria, the cultured colonies System identification and drug susceptibility experiments were performed. The detection time of this traditional detection method usually reaches 72 hours or more, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/38C12R1/385
CPCC12N1/38C12Q1/045
Inventor 宋征奇杨学敏朱克先谭谨刘丽霞刘红霞马晓玉
Owner 廊坊恒益生物技术有限公司
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