Method for preparing sericin film material based on enzymatically grafted vinyl monomer
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A technology of vinyl and membrane materials, applied in the direction of peptides and fermentation, which can solve the problems of low accessibility and achieve the effects of improved formability, high efficiency, and expanded application scope
Active Publication Date: 2017-07-25
JIANGNAN UNIV
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But on the other hand, although HRP can catalyze the generation of phenolic oxygen free radicals from tyrosine residues in sericin, and trigger the graft copolymerization of sericin and vinyl-containing ester monomers, the tyrosine residues in sericin protein The quantity is limited or its accessibility is low in the enzymatic reaction, and it is difficult to carry out efficient graft copolymerization with vinyl-containing ester monomers, so that less ester monomer copolymers are introduced on the surface of sericin after the reaction, that is, silk The water solubility of collagen is still high, and the formability of sericin material still needs to be improved
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Embodiment 1
[0024] Prepare the sericin film material with the method mentioned in the present invention, concrete process step is as follows:
[0025] (1) Preparation of sericin solution: Degumming silk with soda ash, wherein 5g / L soda ash, temperature 95°C, treatment time 1 hour; then dialyzed with deionized water for 24 hours to obtain a sericin solution with a concentration of 20g / L ;
[0026] (2) enzymatic sericin grafting primary amine compounds containing phenolic hydroxyl groups: add transglutaminase and p-hydroxyphenylethylamine to the sericin solution in step (1), wherein transglutaminase 5U / mL, p-hydroxyphenylethylamine 2.5g / L, temperature 40°C, pH 6.5, treatment time 12 hours; then dialyzed in deionized water for 24 hours to remove unreacted p-hydroxyphenylethylamine to obtain a concentration of 20g / The modified sericin solution of L;
[0027] (3) Enzyme-catalyzed graft copolymerization of sericin and vinyl-containing ester monomers: in the sericin solution of step (2), add...
Embodiment 2
[0035] Prepare the sericin film material with the method mentioned in the present invention, concrete process step is as follows:
[0036] (1) Preparation of sericin solution: degumming silk with soda ash to prepare sericin solution;
[0037] Treatment process prescription and conditions: Soda ash 7.5g / L, temperature 95°C, treatment time 0.75 hours; then dialyze with deionized water for 30 hours to obtain a sericin solution with a concentration of 25g / L;
[0038] (2) enzymatic sericin grafting with primary amine compounds containing phenolic hydroxyl groups: adding transglutaminase and dopamine to the sericin solution in step (1) for treatment to increase the number of phenolic hydroxyl groups on the sericin;
[0039] Treatment process prescription and conditions: transglutaminase 8U / mL, dopamine 4g / L, temperature 40°C, pH 6.8, treatment time 8 hours; then dialyze in deionized water for 24 hours to remove unreacted dopamine, and obtain Concentration is the modified sericin solu...
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Abstract
The invention discloses a method for preparing a sericin film material based on an enzymatically grafted vinyl monomer. The method aims at catalyzing the graft copolymerization of sericin and a vinyl-containing ester monomer through an enzyme, decreasing the water solubility of the sericin and improving the moldability of the sericin film material. A phenolic hydroxyl containing primary amine compound is grafted onto sericin protein through a transglutaminase method; the reactivity of the sericin is improved; afterwards, the graft copolymerization of the sericin and the vinyl-containing ester monomer is catalyzed by means of horseradish peroxide; the sericin film material is prepared. The method comprises the following specific processes and steps of (1), preparing a sericin protein solution; (2), enzymatically making the sericin graft the phenolic hydroxyl containing primary amine compound through the enzyme; (3), catalyzing the graft copolymerization of the sericin and the vinyl-containing ester monomer through the enzyme; (4), molding the sericin film material. In comparison with a chemical method or an ultraviolet irradiation method and the like by which the graft copolymerization of the sericin and the vinyl monomer is initiated, the method provided by the invention is high in graft copolymerization efficiency; the enzyme treatment condition is mild; the moldability of the sericin film material is ameliorated.
Description
technical field [0001] The invention relates to a method for preparing sericin membrane material based on enzymatic grafting of vinyl monomers, which is characterized in that a primary amine compound containing phenolic hydroxyl groups is first grafted on sericin by enzymatic method, and then enzymatically catalyzed by enzymatic sericin and The invention relates to graft copolymerization of vinyl-containing ester monomers to prepare sericin film materials, belonging to the field of textile biotechnology. Background technique [0002] Silk is composed of silk fibroin and sericin covering the surface of sericin. Silk fibroin has a linear structure and high crystallinity, and is mostly used in textile fiber processing; silk sericin (SS) is a spherical protein, containing more hydrophilic polar amino acids, and its supramolecular structure is mainly random coils , high water solubility. After the silk is degummed by alkaline method or protease method, the sericin in the outer ...
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