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A antagonistic wood mold and its application that promote the growth of crops

A technology of Trichoderma dark green and inoculum, applied in the field of microorganisms, can solve problems such as damage to wheat growth, environmental pollution, economic losses, etc., and achieve the effects of reducing agricultural production input, reducing ecological environment pollution, and improving yield and quality.

Active Publication Date: 2020-12-22
SHANDONG AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of pesticides is limited by factors such as the amount of pesticides applied, the method of application, and degradation. Chemical control is also likely to cause pathogenic bacteria to develop drug resistance.
[0009] Wheat sheath blight is a common and highly harmful soil-borne disease, which harms the growth of wheat, causes diseases of the roots and even the whole plant of wheat, and causes significant economic losses.
In disease control, chemical pesticide seed dressing has a certain effect on soil-borne diseases. However, the use of chemical pesticides will cause environmental pollution and pesticide residues. At the same time, the long-term and excessive use of chemical pesticides will seriously harm or even destroy the agricultural ecosystem. It kills the beneficial microorganisms in the environment, increases the resistance of plant pathogens, and eventually leads to a decline in the control effect or even a failure of control

Method used

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  • A antagonistic wood mold and its application that promote the growth of crops
  • A antagonistic wood mold and its application that promote the growth of crops
  • A antagonistic wood mold and its application that promote the growth of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 Antagonistic Trichoderma source and screening

[0045] 1. Source of test strain

[0046]In 2012, the root soils of wheat fields were collected from Jining, Dezhou, Liaocheng, Heze, Tai'an, Zibo, Binzhou, Weifang and other areas in Shandong. 2-3 counties were selected for soil collection in each area, with a total of more than 50 samples. 70 strains of Trichoderma were isolated by dilution plate method.

[0047] The 10 excellent Trichoderma strains and T10 strains preserved in the laboratory were presented by friends.

[0048] 2. Isolation of Trichoderma

[0049] 2.1 Collection of soil samples

[0050] For plots with serious incidence of wheat sheath blight and root rot, when sampling, first remove the 1-2cm soil layer on the soil surface and shovel off, then take soil, take three points for each soil, and mix the soil at each point fully After uniformity, put it into a sterile sampling bag, take it back to the laboratory and store it in a refrigerator ...

Embodiment 2

[0131] Embodiment 2 Trichoderma morphology and molecular identification

[0132] 1 Culture identification of Trichoderma morphology

[0133] After the purified Trichoderma was cultured to produce spores, the spores and spore-forming peduncles were picked out, the spores were observed under a microscope, and the original literature was consulted for identification.

[0134] The colony grows fast on the PDA plate, the mycelium layer is thicker, densely bundled, white and flat at the initial stage, and dark green due to the production of conidia in the later stage. The back of the colony is colorless, sometimes pale yellow. The hyphae are transparent, with septa, and the cell wall is smooth. The conidiophores grow upright from the mycelia, with secondary to tertiary branches, and the whole is like a branch; the angle between the branches and the conidiophores is approximately right angle, and the end is a small stalk. Small stem bottle-shaped, conidia spherical or oblong.

[0...

Embodiment 3

[0202] Example 3 Determination of growth-promoting and disease-preventing effects of antagonistic Trichoderma strains

[0203] 1. Trichoderma solid fermentation product and sheath blight inoculum preparation

[0204] (1) Trichoderma solid fermentation product preparation

[0205] Wheat bran: Straw powder: Corn flour is used as the raw material. Trichoderma spore suspension is added to the sterilized material. After one week of fermentation, a large number of green spores are produced, and the spore concentration reaches 1×10 9 cfu / g.

[0206] (2) Preparation of Trichoderma spore suspension

[0207] Weigh a certain amount of the prepared trichoderma solid fermentation product, and dilute it in water to the required concentration according to the required concentration.

[0208] (3) Preparation of sheath blight inoculum

[0209] After steaming the wheat grains for 30 minutes, put them into Erlenmeyer flasks, 200g per bottle, 2g of glucose, autoclave at 121°C for 20min, and w...

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Abstract

The use of chemical pesticides to prevent and control agricultural diseases will cause environmental pollution, pesticide residues, harm the agricultural ecosystem, kill beneficial microorganisms in the environment, increase the resistance of plant pathogens, and ultimately lead to reduced control effects or even failure of control. The present invention provides a kind of antagonistic Trichoderma for promoting the growth of crops, the preservation number is CGMCC No.9774 and 9775 Trichoderma dark green ( Trichoderma atroviride ) H18‑1‑1 and TF28. The Trichoderma provided by the present invention can not only be applied in agricultural biological control, but also can promote the growth of crops such as wheat, and can prevent and treat Rhizoctonia graminearum, Fusarium flavus, Fusarium explantarum and / or Fusarium oxysporum caused by disease.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an antagonistic trichoderma for promoting the growth of crops and its application. Background technique [0002] Rhizoctonia graminearum, Latin name Rhizoctonia cerealis , a fungus belonging to the order Asposporales. It can cause sheath blight of wheat, barley, corn, and rice. It is a soil-borne fungal disease that commonly occurs in wheat-growing areas in my country and is seriously harmful. [0003] Wheat sheath blight caused by Rhizoctonia graminearum infection is a worldwide disease. In nearly 20 provinces (cities) in my country, there are different degrees of occurrence and damage. In Jiangsu, Zhejiang, Anhui, Shandong, Henan, Shaanxi, Guizhou, Hubei and Sichuan provinces, the occurrence is common, the damage is serious, and the output loss is light. 5%-10%, and severe cases will reduce production by 20%-40%. Even cause dry pregnant (white) ears and no grain har...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/38A01P3/00A01P21/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 于金凤吴江南徐文凤张莉刘春菊杜传印毛志全
Owner SHANDONG AGRICULTURAL UNIVERSITY
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