Method for preparing carbon dot from mushroom and carbon dot prepared by using method and application

A technology of carbon dots and mushrooms, which is applied in the field of preparation of luminescent materials, can solve the problems that have not yet been seen, and achieve the effects of good fluorescence performance, good application prospect and good biocompatibility.

Inactive Publication Date: 2017-08-15
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But so far, there is no report on the preparation of carbon dots using mushrooms as carbon sources.

Method used

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  • Method for preparing carbon dot from mushroom and carbon dot prepared by using method and application
  • Method for preparing carbon dot from mushroom and carbon dot prepared by using method and application
  • Method for preparing carbon dot from mushroom and carbon dot prepared by using method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1. Preparation of carbon dots with mushrooms as carbon source

[0027] 1. Preparation of carbon dots

[0028] Such as figure 1 As shown, fresh mushrooms were dried at 80°C for 24 hours, and then ground into powder with a mortar; take 0.6 g of mushroom powder, add 6 mL of ultrapure water, mix well, put in a 25 mL polytetrafluoroethylene stainless steel reaction kettle, 200 React at ℃ for 6 hours; cool the resulting reaction solution to room temperature, centrifuge at 10,000 rpm for 15 minutes, collect the supernatant, filter it with a microporous membrane with a pore size of 0.22 μm, and dialyze the filtrate with a dialysis bag with a molecular weight cut-off (MWCO) of 100 for 24 hours Afterwards, freeze-dry to obtain carbon dot powder; disperse the carbon dot powder with ultrapure water to obtain a 1 μg / mL carbon dot dispersion, and store at 4°C.

[0029] 2. Characterization of carbon dots

[0030] The optimal excitation wavelength of carbon dots is 370 nm, a...

Embodiment 2

[0045] Example 2, optimization of conditions for preparing carbon dots with mushrooms as carbon source

[0046] 1. Response time optimization

[0047] According to the method described in Example 1, carbon dots were prepared by reacting at 200° C. for 4, 5, 6, 7, 8, and 9 hours, respectively. The result is as Figure 8 As shown in A, the fluorescence effect of carbon dots prepared by reacting for 5-7 hours is better, and the best reaction time is 6 hours.

[0048] 2. Reaction temperature optimization

[0049] According to the method described in Example 1, carbon dots were prepared by reacting at 120, 140, 160, 180, and 200° C. for 6 hours, respectively. The result is as Figure 8 As shown in B, the fluorescence effect of the carbon dots prepared by the reaction at 180-200°C is better, and the best reaction temperature is 200°C.

Embodiment 3

[0050] Example 3. Application of carbon dots prepared from mushrooms as a carbon source in cell imaging

[0051] Inoculate human lung adenocarcinoma cells (A549 cells) into 6 wells of a 96-well plate, add blocking solution to each well, put them in a wet box, and then put them in a 37°C, 5% CO 2 Incubate in the incubator for 30 minutes, take out the 96 microwell plate, first wash off the blocking solution with 0.01mol / L phosphate buffer solution, then use filter paper to dry up the liquid around the orifice plate, and the carbon dot dispersion prepared in Example 1 with A549 cells in 6 wells at 37°C, 5% CO 2 Incubate for 1 hour in the incubator, wash with 0.01mol / L phosphate buffer, and then observe the labeling of the cells under a fluorescence microscope.

[0052] The results of carbon dot labeling of A549 cells are as follows: Figure 9 As shown, it can be seen that A549 cells exhibit obvious green fluorescence under a fluorescent microscope, indicating that the carbon do...

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Abstract

The invention discloses a method for preparing a carbon dot from mushroom. The method comprises the steps of mixing mushroom powder and water at the ratio of (0.08g-0.1g):1mL, and then reacting at 180-200 DEG C for 5-7h to prepare the carbon dot. A native plant resource mushroom widespread in the natural world is utilized as a carbon source, and the carbon dot is prepared through a simple hydrothermal method, so that the method is green and environment-friendly, and the obtained carbon dot has the advantages of good fluorescence property, good light stability, low toxicity and good biocompatibility and has a good application prospect in the aspect of cell imaging.

Description

technical field [0001] The invention belongs to the technical field of preparation of luminescent materials, relates to a method for preparing carbon dots from mushrooms, and also relates to the carbon dots prepared by the method and the application of the carbon dots. Background technique [0002] In recent years, as a new and important member of the family of carbon nanomaterials, carbon dots have received more and more attention. In addition to having excellent optical properties similar to traditional semiconductor quantum dots, carbon dots also have good photostability, low toxicity, good biocompatibility and environmental friendliness, mild preparation conditions, simple steps, abundant and cheap raw materials, etc. The incomparable advantages of quantum dots. At present, the research of carbon dots focuses on finding a simple preparation method and its application in fluorescence analysis. [0003] The synthesis of carbon dots is generally divided into top-down meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/15C09K11/65G01N21/64
CPCC01P2002/70C01P2002/82C01P2004/04C09K11/65G01N21/6486
Inventor 杨晓明杨焜诚王颖怡王珊珊刘明旭付彬
Owner SOUTHWEST UNIVERSITY
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