Fluorescence probe based on BODIPY type singlet oxygen

Abstract

The invention discloses a fluorescence probe based on BODIPY type singlet oxygen. The fluorescence probe has the characteristics of high sensitivity, high selectivity and fast response in a neutral environment for detection of singlet oxygen. The fluorescence probe has no fluorescence, fluorescence is obviously enhanced after response with the singlet oxygen, the fluorescence intensity and the concentration of the singlet oxygen have good linear relation, the fluorescence probe can be used for quantitative detection, and other active oxygen substances do not interfere detection. The prepared fluorescence probe has classic BODIPY spectral characteristic, can detect mercury ions in chemical samples, biological samples or medical samples on the basis of fluorescence enhancement or chromaticity enhancement, and is suitable for fluorescence imaging.

Description

technical field

[0001] The invention belongs to the field of fluorescent probes and specifically designs a preparation method and application of an enhanced singlet oxygen fluorescent probe based on BODIPY fluorescent dye. Background technique

[0002] In biological systems, singlet oxygen ( 1 o 2 ) is an important reactive oxygen species. 1 o 2 With high chemical activity and large diffusion radius, it can penetrate the mitochondrial membrane or nuclear membrane and cause DNA damage; it can interact with histones, and histidine residues pass through 1 o 2 Oxidation produces a new photoproduct. Moreover, its amount is directly proportional to cell damage and death; it can oxidize cysteine, histidine, tryptophan, methionine and tyrosine residues, making proteins lose their function; it can make enzyme protein molecules undergo intramolecular Or intermolecular cross-linking, leading to configuration changes and inactivation or exposing some hidden peptide bonds and being...

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