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Rapid aquaculture water nitrite detection kit

A detection kit and nitrite technology, which is applied in the direction of material analysis by observing the impact on chemical indicators, and analysis by making materials undergo chemical reactions, can solve the problem of uncontrollable powder usage, deterioration of reagent quality, detection Inaccurate results and other problems, to achieve the effect of increasing the effective shelf life, maintaining physical and chemical properties, and improving sensitivity

Active Publication Date: 2017-09-05
福建大北农华有水产科技集团有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In intensive aquaculture water bodies, the stocking density of aquatic animals is high, and the amount of bait fed is large. A large amount of nitrogen-containing organic matter such as residual bait, animal and plant excreta, and dead remains is easily accumulated in the aquaculture water body. In addition, untreated aquaculture Wastewater and industrial and domestic sewage are discharged or washed by rainwater into the aquaculture water body, resulting in serious excess of nitrite in the aquaculture water body
[0003] Excessive nitrite can easily lead to a large number of harmful bacteria and conditional pathogens, and the physical fitness of fish, shrimp and other farmed animals will decline, and their anti-stress ability will be poor. cause huge economic losses
[0004] Although the common water quality nitrite detection kits on the market are easy to operate and easy to carry, they have poor anti-interference ability, are prone to false positive and false negative results, and have low sensitivity.
And most of them are powder or a combination of various reagents. Farmers often encounter problems such as uncontrollable powder usage and deterioration of reagent quality during the detection process, which makes the detection results inaccurate; sometimes more detection reagents are used. It is cumbersome, and the test results are prone to large errors if you do not pay attention to it
Chinese patents CN 102507553A, CN103743727A, and CN 104407097 are all kits for detecting nitrite content in aquaculture water. They are all mixed with ordinary chemical indicators. The former reagent has cumbersome steps and poor reagent stability, while the latter two The color development time of the reagent is a bit long

Method used

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  • Rapid aquaculture water nitrite detection kit
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  • Rapid aquaculture water nitrite detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Weigh each raw material in parts by mass: 45 parts of p-aminobenzenesulfonic acid, 40 parts of naphthaleneethylenediamine hydrochloride, 85 parts of hydrochloric acid, 30 parts of cetyltrimethylammonium bromide, 25 parts of disodium edetate Parts, fully dissolved in 60 parts of distilled water, that is.

[0026] Kit assembly: 1 bottle of test reagent, 1 colorimetric bottle and 1 colorimetric card.

[0027] The specific operation steps are as follows:

[0028] 1) Use the water sample to be tested to rinse the colorimetric bottle 2 to 3 times;

[0029] 2) Draw the water sample to be tested into the colorimetric bottle, and set the volume to 15mL;

[0030] 3) Add 5 drops of the test agent dropwise, cover the colorimetric bottle, and compare it with the standard colorimetric card after shaking well, find out the color block with similar color, which is the nitrite content in the water sample to be tested.

Embodiment 2

[0032] The raw materials were weighed in parts by mass: 40 parts of p-aminobenzenesulfonic acid, 35 parts of naphthaleneethylenediamine hydrochloride, 60 parts of acetic acid, 20 parts of citric acid, 15 parts of cetyltrimethylammonium bromide, octadecane 10 parts of trimethylammonium bromide (STAB), 10 parts of monoethanolamine, 15 parts of triethanolamine, fully dissolved in 85 parts of distilled water, to obtain.

[0033] Kit assembly: 1 bottle of test reagent, 1 colorimetric bottle and 1 colorimetric card.

[0034] The specific operation steps are as follows:

[0035] 1) Use the water sample to be tested to rinse the colorimetric bottle 2 to 3 times;

[0036] 2) Draw the water sample to be tested into the colorimetric bottle, and set the volume to 15mL;

[0037] 3) Add 5 drops of the test agent, cover the colorimetric bottle, and compare it with the standard colorimetric card after shaking well, and find out the color block with similar color, which is the nitrite conten...

Embodiment 3

[0039] Weigh each raw material by mass parts: 25 parts of p-aminobenzenesulfonic acid, 30 parts of naphthaleneethylenediamine hydrochloride, 75 parts of acetic acid, 20 parts of cetyltrimethylammonium bromide, 15 parts of polyvinyl alcohol, fully dissolve In 80 parts of distilled water, that is.

[0040] Kit assembly: 1 bottle of test reagent, 1 colorimetric bottle and 1 colorimetric card.

[0041] The specific operation steps are as follows:

[0042] 1) Use the water sample to be tested to rinse the colorimetric bottle 2 to 3 times;

[0043] 2) Draw the water sample to be tested into the colorimetric bottle, and set the volume to 15mL;

[0044] 3) Add 5 drops of the test agent, cover the colorimetric bottle, and compare it with the standard colorimetric card after shaking well, and find out the color block with similar color, which is the nitrite content in the water sample to be tested.

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PUM

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Abstract

The invention discloses a rapid aquaculture water nitrite detection kit which comprises one bottle of testing agent, a color comparison bottle and a color comparison card. The testing agent comprises p-aminobenzene sulfonic acid, N-ethylenediamine dihydrochloride, acids, surface active agents, stabilizers and distilled water. The kit has the advantages that the kit is rapid, sensitive, convenient and rapid to use, stable in quality and the like, the kit solves the problems that a conventional detection kit is complex in operation step in the detection process or the agents go bad and changes colors as the agents are placed for a long time and the like, the kit is simple and efficient in operating step and applicable to rapid on-site detection of various water without adding any devices.

Description

technical field [0001] The invention belongs to the field of water body detection, and in particular relates to a rapid detection kit for nitrite in aquaculture water. Background technique [0002] In intensive aquaculture water bodies, the stocking density of aquatic animals is high, and the amount of bait fed is large. A large amount of nitrogen-containing organic matter such as residual bait, animal and plant excreta, and dead remains is easily accumulated in the aquaculture water body. In addition, untreated aquaculture Wastewater and industrial and domestic sewage are discharged or washed by rainwater into the aquaculture water body, resulting in a serious excess of nitrite in the aquaculture water body. [0003] Exceeding the standard of nitrite can easily lead to a large number of harmful bacteria and conditional pathogenic bacteria, and the physical fitness of fish, shrimp and other farmed animals will decline, and their anti-stress ability will be poor. cause huge ...

Claims

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Application Information

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IPC IPC(8): G01N21/79
CPCG01N21/79
Inventor 黄广富秦波王宁朱传忠
Owner 福建大北农华有水产科技集团有限公司
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