Microbial remediation method for cadmium-polluted bottom mud

A technology for microbial remediation and cadmium pollution, applied in the restoration of polluted soil, etc., can solve the problems of rare heavy metal pollution sediment restoration research, poor composite pollution restoration effect, and poor field test effect, etc. Small, low cost, low mobility effect

Active Publication Date: 2017-09-08
BEIHANG UNIV
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  • Application Information

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Problems solved by technology

However, judging from the current research status, there are not many studies on the application of SRB to heavy metal remediation in sediment. When it is used for heavy metal remediation, it still has the shortcomings of low stabilization efficiency for individual metals and poor remediation effect for composite pollution; Sporadic studies mostly focus on water and soil, while researches on remediation of heavy metal-contaminated sediments are rare and less applied. One of the most important reasons is the environmental conditions (such as pH, ORP, sulfuric acid, etc.) required for the growth and metabolism of SRB. Salt, temperature, stable electron donors, etc.) are difficult to have both under natural conditions, resulting in a remarkable effect of SRB indoor restoration, but poor field test results

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  • Microbial remediation method for cadmium-polluted bottom mud
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Embodiment 1

[0042] The microbial remediation method of Cd polluted bottom mud is provided in the present embodiment, specifically comprises the following two steps:

[0043] Step 1: Enrichment, domestication and expansion of sulfate-reducing bacteria

[0044] (1) Medium: KH 2 PO 4 0.5g / L, NH 4 Cl 1g / L, CaSO 4 1g / L, MgSO 4 ·7H 2 O 2g / L, lactic acid 3.5g / L, yeast extract 1g / L, ascorbic acid 0.1g / L, thioglycolic acid 0.1g / L, FeSO 4 ·7H 2 O 0.5g / L, ultrapure water 1L, adjust the pH between 7.0 and 7.5.

[0045] (2) Strain enrichment: pick 2g of reservoir natural sediment samples, put them into a 50mL stoppered glass tube, add 9mL of 0.75% (mass percentage concentration) of normal saline to obtain 10 -1diluent. After shaking well, draw 2mL of the bottom mud dilution and add it into a sterilized Erlenmeyer flask containing 300mL of medium. Seal the Erlenmeyer flask with parafilm, and place it in a constant temperature incubator at 30°C for cultivation. After 2 to 3 days, it will be...

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Abstract

The invention discloses a microbial remediation method for cadmium-polluted bottom mud, and belongs to the field of microbial remediation of the bottom mud. The method comprises the following steps: enriching sulfate reducing bacteria, domesticating the sulfate reducing bacteria and expanding culture of the sulfate reducing bacteria; and repairing the Cd-polluted bottom mud by using the sulfate reducing bacteria. According to the method, the sulfate reducing bacteria and compound bacteria are added into the Cd-polluted bottom mud after separation identification, the content of the exchangeable form and the carbonate binding form of the Cd can be reduced, the content of the iron-manganese oxide binding form, the organic matter binding form and the residue form can be increased, so that the Cd is converted into a stable form, the content of the Cd in pore water of the repaired bottom mud is obviously reduced, and therefore the mobility, the biological effectiveness and the toxicity of the Cd are reduced. The method has the characteristics of being simple in technology, low in cost, good in remediation effect and large in in-situ application potential and the like.

Description

technical field [0001] The invention belongs to the field of bottom mud microbial restoration, and in particular relates to a microbial restoration method for cadmium-contaminated bottom mud. Background technique [0002] Heavy metals have attracted much attention due to their ecological and environmental effects such as refractory, bioaccumulation, and food chain amplification. Water body sediment is the final destination of various pollutants after entering the water environment. More than 99% of heavy metals will accumulate in the sediment in various forms after entering the water environment, making the heavy metal content of the sediment often several orders of magnitude higher than that of the overlying water. resulting in sediment pollution. Worldwide, heavy metal pollution is common in water sediments, especially cadmium (Cd), which is highly toxic. For example, the Cd in the surface sediment of Keratsini Port, the most industrially developed Attica in Greece, is as...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
CPCB09C1/10
Inventor 范文宏彭位华
Owner BEIHANG UNIV
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