Preparation method of natural spice gamma decalactone

A technology of natural flavor and decolactone, which is applied in the fields of materials science and biological fermentation engineering, can solve the problems of lower utilization rate of yeast to substrates, increased one-time investment cost of γ-decalactone, unfavorable yeast cell activity, etc. Improve product formation rate, good industrial application prospects, and increase the effect of one-time accumulation

Inactive Publication Date: 2017-09-12
SHANGHAI INST OF TECH
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AI Technical Summary

Problems solved by technology

[0003] Using ricinoleic acid as a substrate, the production process of gamma-decalactone prepared by fermentation is the biggest problem at present: because ricinoleic acid is insoluble in water, its dispersibility in water is poor. As a result, the transport process of ricinoleic acid molecules in the aqueous medium to the yeast cells is greatly restricted, and the contact between the yeast and the substrate is insufficient, resulting in a decrease in the utilization rate of the substrate by the yeast, and finally seriously affecting the γ-decalactone. The production rate and output of
According to current relevant literature and patent reports, in order to solve the problems existing in the above-mentioned fermentation process, two methods are generally used to solve them: (1) adding surfactants to the fermentation broth to enhance the dispersion of the substrate in the water phase, Although the addition of surfactants is beneficial to the dispersion of substrates in the water phase

Method used

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  • Preparation method of natural spice gamma decalactone
  • Preparation method of natural spice gamma decalactone
  • Preparation method of natural spice gamma decalactone

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1 Control experiment

Embodiment 2

[0031] Example 2 The effect of substrate embedding on the conversion of γ-decalactone

[0032]Treatment of modified fiber materials: crush the modified fiber (purchased from Shanghai Guyang Industrial Co., Ltd., model: GY131) with a pulverizer, use 20, 50, 80, and 120 mesh sieves to screen the fibers, and filter <20 mesh, 20- 50 mesh, 50-80 mesh, and 80-120 mesh fiber particles were screened out; embedding: Mix the screened modified fibers with different meshes and ricinoleic acid at a mass ratio of 1:1, and bathe in water at 50°C for 1 hour. And keep stirring; transformation medium preparation: Magnesium sulfate heptahydrate 10g / L, dipotassium hydrogen phosphate 10g / L, glycerol 15g / L, L-carnitine 0.12g / L, pH is 6.4, put the culture medium into 250ml shake In the bottle, the amount of liquid is 50ml; the addition of embeddings: based on ricinoleic acid 40g / L, the amount of embeddings is 4g; sterilization: 121°C, damp heat sterilization for 15min; inoculation: after the bacteri...

Embodiment 3

[0033] Example 3 Biological Fermentation Tank Transformation Control Experiment

[0034] Preparation of transformation medium: ricinoleic acid 40g / L, magnesium sulfate heptahydrate 10g / L, dipotassium hydrogen phosphate 10g / L, glycerin 15g / L, L-carnitine 0.12g / L, pH 6.4, 5L canned liquid Amount of 3L; Sterilization: 121 ℃, damp heat sterilization for 15min; Inoculation: After sterilization and cooling, according to the yeast powder 15g / L, weigh high-activity yeast powder (purchased from Angel Yeast Co., Ltd. Company, model: food grade) 45g, and put it into a fermenter; transformation culture: stirring speed 400r / min, temperature 28°C, ventilation volume 4.0v ▪v -1 ▪ m -1 , the conversion time was 90 hours, and the fermentation broth containing γ-decalactone was obtained; detection: when sampling, increase the speed to 600 r / min, take 10ml with a pipette, add three times the volume of absolute ethanol, and shake well , sampled and processed for detection by gas chromatography ...

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Abstract

The invention provides a preparation method of a natural spice gamma decalactone. The preparation method comprises the steps of smashing modified fiber with a crusher, screening with sieves of different meshes, mixing the screened modified fiber with castor oil acid, bathing in water and stirring to obtain an embedded substance; preparing a transformation medium, adding the embedded substance into the transformation medium according to a certain quantity, and performing moist-heat sterilization on the transformation medium containing the embedded substance; and adding highly active yeast powder into the transformation medium containing the embedded substance, then transforming by shaking a flask and transforming in a fermentation tank, and after transformation is completed, obtaining the gamma decalactone. Compared with a conventional oil-water two-phase fermentation system, the method can help improve the growth rate of the product and shorten the fermentation period, and can relieve suppression of the substrate castor oil acid on yeast cells, so that the cell activity reaches a higher level, and the accumulation quantity of gamma decalactone for one time is increased substantially.

Description

technical field [0001] The invention belongs to the fields of materials science and biological fermentation engineering, and relates to a fiber material, in particular to a preparation method of natural flavor gamma-decalactone. Background technique [0002] γ-Decalactone (Gamma decalactone), referred to as GDL, is a lactone fragrance substance with peach fragrance. It is a ten-carbon compound containing a five-membered lactone ring and is a colorless liquid with the molecular formula C 10 h 18 o 2 , with a molecular weight of 170.25, slightly soluble in water and easily soluble in organic solvents, is widely used in the preparation of cream, peach, citrus and coconut flavors, and is one of the most demanding products in flavors. With the wide application of γ-decalactone in the fragrance and flavor industry, many fragrance companies have turned their interest in this potential market and started research and development, which has led to the rapid development of the biot...

Claims

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Application Information

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IPC IPC(8): C12P17/04C12R1/645
CPCC12P17/04
Inventor 荣绍丰王梦泽管世敏张硕李智弟
Owner SHANGHAI INST OF TECH
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