Preparation method of natural spice gamma decalactone

Abstract

The invention provides a preparation method of a natural spice gamma decalactone. The preparation method comprises the steps of smashing modified fiber with a crusher, screening with sieves of different meshes, mixing the screened modified fiber with castor oil acid, bathing in water and stirring to obtain an embedded substance; preparing a transformation medium, adding the embedded substance into the transformation medium according to a certain quantity, and performing moist-heat sterilization on the transformation medium containing the embedded substance; and adding highly active yeast powder into the transformation medium containing the embedded substance, then transforming by shaking a flask and transforming in a fermentation tank, and after transformation is completed, obtaining the gamma decalactone. Compared with a conventional oil-water two-phase fermentation system, the method can help improve the growth rate of the product and shorten the fermentation period, and can relieve suppression of the substrate castor oil acid on yeast cells, so that the cell activity reaches a higher level, and the accumulation quantity of gamma decalactone for one time is increased substantially.

Description

technical field [0001] The invention belongs to the fields of materials science and biological fermentation engineering, and relates to a fiber material, in particular to a preparation method of natural flavor gamma-decalactone. Background technique [0002] γ-Decalactone (Gamma decalactone), referred to as GDL, is a lactone fragrance substance with peach fragrance. It is a ten-carbon compound containing a five-membered lactone ring and is a colorless liquid with the molecular formula C 10 h 18 o 2 , with a molecular weight of 170.25, slightly soluble in water and easily soluble in organic solvents, is widely used in the preparation of cream, peach, citrus and coconut flavors, and is one of the most demanding products in flavors. With the wide application of γ-decalactone in the fragrance and flavor industry, many fragrance companies have turned their interest in this potential market and started research and development, which has led to the rapid development of the biot...

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Problems solved by technology

[0003] Using ricinoleic acid as a substrate, the production process of gamma-decalactone prepared by fermentation is the biggest problem at present: because ricinoleic acid is insoluble in water, its dispersibility in water is poor. As a result, the transport process of ricinoleic acid molecules in the aqueous medium to the yeast cells is greatly restricted, and the contact between the yeast and the substrate is insufficient, resulting in a decrease in the utilization rate of the substrate by the yeast, and finally seriously affecting the γ-decalactone. The production rate and output of

According to current relevant literature and patent reports, in order to solve the problems existing in the above-mentioned fermentation process, two methods are generally used to solve them: (1) adding surfactants to the fermentation broth to enhance the dispersion of the substrate in the water phase, Although the addition of surfactants is beneficial to the dispersion of substrates in the water phase and the transport rate of substrates into cells, it is not conducive to the normal growth of cells, and even high concentrations of surfactants can also inhibit the activity of yeast cells. , so that the production of γ-decalactone cannot be greatly improved; (2) Add high-concentration ricinoleic acid to the fermentation broth for biotransformation

The addition of high-concentration ricinoleic acid helps to increase the effective concentration of the substrate in the water phase, and can increase the production of γ-decalactone within a certain period of time, but too high a concentration of ricinoleic acid in the water phase has a relatively negative effect on yeast cells. The obvious inhibitory effect is not conducive to the maintenance of yeast cell activity, so that the final total output of γ-decalactone is not greatly improved, and a large amount of substrate addition increases the one-time input cost of γ-decalactone production. Not conducive to industrial scale-up of products

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