Selenium-enriched microorganism and preparation and application method thereof
A technology of selenium-enriched microorganisms and microorganisms, applied in the biological field, can solve the problems of poor viability, inconvenient storage and use of probiotics, etc.
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Embodiment 1
[0167] Example 1 Using continuous culture to obtain selenium-enriched probiotics adapted to high-selenium medium
[0168] 1.1 Training method 1:
[0169] Inoculate Bifidobacterium longum in RCM liquid medium, the inoculum volume ratio, that is, the volume ratio of seed liquid to RCM liquid medium is 1%, anaerobic culture at 37±1°C for 8h, it is a first-class seed, and every 10 Parts of RCM medium are arrayed in parts by weight: about 0.1 part of beef extract, about 0.05 part of peptone, about 0.03 part of yeast powder, about 0.05 part of glucose, about 0.01 part of starch, about 0.05 part of sodium chloride, about 0.03 part of sodium acetate, About 0.005 part of L-cysteine hydrochloride, the rest is water. Inoculate the first-grade seed liquid in a 2L continuous culture device according to the volume ratio of the inoculum volume. The volume of the medium is 50%, and the nitrogen gas is continuously passed to maintain anaerobic conditions. After culturing at 37±1°C for 16 ho...
Embodiment 2
[0182] Example 2: Antioxidant test of probiotics cultured with enriched selenium
[0183] In the experimental group, using the method 1.1 of the above-mentioned Example 1, selenium-enriched Bifidobacterium longum was cultured in selenium-containing medium with different final concentrations, and anaerobically cultured at each final concentration for 24 hours. The final concentration of selenium in the medium of each experimental group (in the form of Na added in the medium 2 SeO 3 Concentration meter) are: 10 μg / mL, 20 μg / mL, 30 μg / mL, 40 μg / mL, 50 μg / mL, 60 μg / mL, 70 μg / mL, 80 μg / mL, 90 μg / mL, 100 μg / mL, 200 μg / mL.
[0184] Selenium was not added to the culture medium of the control group, and other culture conditions were the same as those of the experimental group.
[0185] Centrifuge, wash, and resuspend the above-mentioned anaerobic cultures for 24 hours, then transfer them to aerobic conditions and culture them with shaking at 37°C in a selenium-free fresh medium, take...
Embodiment 3
[0187] Example 3 Selenium-enriched bifidobacteria are improving the tolerance of bifidobacteria to antibiotics
[0188] According to the method 1.1 in Example 1, selenium-enriched Bifidobacterium longum was cultivated in selenium-containing medium with different final concentrations to obtain the thalli cultivated under different selenium concentration conditions:
[0189] Control group: fermented cells of Bifidobacterium longum common (no selenium added to the culture medium)
[0190] Experimental group 1: selenium-enriched bacteria fermented with a final concentration of sodium selenite in the culture medium of 10 μg / ml;
[0191] Experimental group 2: selenium-enriched bacteria obtained from fermentation with a final concentration of sodium selenite in the medium of 20 μg / ml;
[0192] Experimental group 3: selenium-enriched bacteria obtained from fermentation with a final concentration of sodium selenite in the medium of 100 μg / ml;
[0193] Using drug-sensitive paper sheet...
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