Cobalt-ion-doped iron-nickel magnetic microsphere and application thereof

A magnetic microsphere, cobalt ion technology, applied in ion exchange, ion exchange regeneration, ion exchange treatment device and other directions, can solve the problems of high price of agarose gel, large production cost, low production cost, etc., and achieve application effect Fast and efficient, easy large-scale industrial production, low cost effect

Inactive Publication Date: 2017-09-19
DALIAN NATIONALITIES UNIVERSITY
View PDF4 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention provides a cobalt-doped iron-nickel magnetic Microspheres can directly adsorb histidine-tagged proteins, and the magnetic microspheres are prepared by a one-pot method based on the principle of thermal decomposition. Cobalt Ion Doped FeNi Magnetic Microspheres

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cobalt-ion-doped iron-nickel magnetic microsphere and application thereof
  • Cobalt-ion-doped iron-nickel magnetic microsphere and application thereof
  • Cobalt-ion-doped iron-nickel magnetic microsphere and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Mix cobalt nitrate and nickel sulfate at a molar ratio of 1:4 (recorded as 20%, indicating that cobalt ions account for 20% of the total moles of cobalt ions and nickel ions), and then mix with iron ions, fully grind and mix for doping Preparation of FeNi magnetic spheres with cobalt ions. The preparation of magnetic balls is based on the principle of thermal decomposition method, and the preparation is completed by a one-pot method. The mixed metal ion salt is added to the ethylene glycol solution at 200 °C under the protection of nitrogen (the oxygen has been fully deoxidized, and sodium acetate and ethanolamine are added to stir. Uniform), keep stirring and reacting for 6h under the protection of nitrogen, then cool to room temperature under the protection of nitrogen and stop the reaction; the synthetic product is washed 5 times with deionized water and 5 times with ethanol solution to obtain 20% cobalt ion-doped Iron nickel magnetic ball.

Embodiment 2

[0037] Mix cobalt nitrate and nickel sulfate according to the molar ratio of ions at 1:3 (recorded as 25%, indicating that cobalt ions account for 25% of the total moles of cobalt ions and nickel ions), and then mix with iron ions, grind and mix thoroughly for use in Preparation of iron-nickel magnetic balls doped with cobalt ions. The preparation of magnetic balls is based on the principle of thermal decomposition method, and the preparation is completed by a one-pot method. The mixed metal ion salt is added to the ethylene glycol solution at 200 °C under the protection of nitrogen (the oxygen has been fully deoxidized, and sodium acetate and ethanolamine are added to stir. homogeneous), keep stirring and reacting for 6h under the protection of nitrogen, then cool to room temperature under the protection of nitrogen and stop the reaction; the synthetic product is washed 7 times with deionized water and 15 times with ethanol solution to obtain 25% doped cobalt ion Iron nickel ...

Embodiment 3

[0039] Mix cobalt nitrate and nickel sulfate according to the molar ratio of ions at 3:7 (recorded as 30%, indicating that cobalt ions account for 30% of the total molar number of cobalt ions and nickel ions), and then mix with iron ions, fully grind and mix for use Preparation of iron-nickel magnetic balls doped with cobalt ions. The preparation of magnetic balls is based on the principle of thermal decomposition method, and the preparation is completed by a one-pot method. The mixed metal ion salt is added to the ethylene glycol solution at 200 °C under the protection of nitrogen (the oxygen has been fully deoxidized, and sodium acetate and ethanolamine are added to stir. uniform), keep stirring and reacting for 6h under the protection of nitrogen, then cool to room temperature under the protection of nitrogen and stop the reaction; the synthesized product is washed 6 times with deionized water and 10 times with ethanol solution to obtain 30% cobalt ion-doped Iron nickel mag...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a cobalt-ion-doped iron-nickel magnetic microsphere and application thereof and belongs to the technical field of chemical materials. The cobalt-ion-doped iron-nickel magnetic microsphere is mainly characterized in that cobalt ions and nickel ions are mixed and used in a magnetic sphere preparation process, the cobalt ions accounts for 20 to 30% of the total mole number of the cobalt ions and the nickel ions, and optimally, the cobalt ions accounts for 25% of the total mole number of the cobalt ions and the nickel ions. The synthesized cobalt-ion-doped iron-nickel magnetic microsphere disclosed by the invention can be directly applied to purifying target protein with a histidine tag, and other modification like antibody modification on the surface of the magnetic sphere is avoided; furthermore, the cobalt-ion-doped iron-nickel magnetic microsphere has good dispersion and quick and high-efficiency application effect and is superior to a no-cobalt-doped iron-nickel magnetic microsphere; separation performance of the cobalt-ion-doped iron-nickel magnetic microsphere is similar to that of imported coordinate-bond nickel-ion sepharose gel; the cobalt-ion-doped iron-nickel magnetic microsphere further can achieve magnetic separation and can replace imported separation materials.

Description

technical field [0001] The invention relates to magnetic microspheres, in particular to an iron-nickel magnetic microsphere doped with cobalt ions and an application thereof. Background technique [0002] Magnetic polymer microsphere is a new type of magnetic material developed in recent years. It not only has many characteristics of ordinary polymer microspheres but also has magnetic responsiveness. It can not only endow its surface with functional groups through copolymerization and surface modification, but also It has a guiding function under the action of an external magnetic field. At present, magnetic composite microspheres have been widely used in many fields such as biomedicine, cytology and separation engineering. [0003] Histidine tags have been widely used in the production process of proteins. Using agarose gel with coordination and binding nickel ions can quickly and highly selectively adsorb histidine-tagged proteins, which improves the separation and purifi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/28B01J20/06B01J20/02B01J20/30B01D15/08
CPCB01J20/28009B01D15/08B01J20/0229B01J20/0281B01J20/0288B01J20/0296B01J20/06B01J20/28019
Inventor 刘宝全李林张艳梅李春斌权春善范圣第
Owner DALIAN NATIONALITIES UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap