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Method for extracting total flavones in kudzu stems

A technology of total flavonoids and kudzu, which is applied to pharmaceutical formulations, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of poor production process safety, incomplete material degradation, and many impurities in the extract, and achieves low cost. , shorten the extraction time, and formulate simple effects

Inactive Publication Date: 2017-09-29
湖北省农业科学院中药材研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many extraction processes for kudzu flavonoids, some of which are directly extracted by aqueous solution, but the degradation of materials in this method is not complete, the extraction rate is very low, and there are many impurities mixed in the extract, which is not conducive to the later stage. Purification and packaging and other treatments; others are treated by adding enzymes such as cellulase and pectinase during the production process, but the disadvantages of this method are high production costs, poor production process safety, and if the added enzymes are removed Incomplete, it will also affect the quality of later products
At the same time, because the extraction of the total flavonoids of Pueraria mirifica is mostly concentrated on the root of Pueraria mirifica, the utilization rate of other parts such as the stem of Pueraria mirifica is less

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting total flavonoids in Pueraria mirifica stems of the present invention comprises the following steps:

[0020] 1. Material collection: collect the Pueraria mirifica stems with a diameter of 2.5 cm and no pests and diseases for later use; use pruning shears to cut the collected Pueraria mirifica stems into 5 cm sections, dry, and fully crush them for later use.

[0021] 2. Dissolution extraction: pass the dried and fully pulverized kudzu stems through a 100-mesh sieve, add NaOH total flavonoids extract with a pH of 9 according to the ratio of solid to liquid ratio of 1:30, and extract for 2 hours, then Heated in a microwave oven with a power of 700 watts for 50 seconds, and then subjected to suction filtration and concentration under reduced pressure to the extract.

[0022] 3. Eluting and drying: adjust the treated extract to neutral pH with dilute hydrochloric acid, pass through an 80-mesh polyamide powder column, elute with 90% ethanol, freeze an...

Embodiment 2

[0025] A method for extracting total flavonoids in Pueraria mirifica stems of the present invention comprises the following steps:

[0026] 1. Material collection: collection of Pueraria mirifica stems with a diameter of 2.0 cm and no pests and diseases for future use. Use pruning shears to cut the collected kudzu stems into 6cm sections, dry them, and fully crush them for later use.

[0027] 2. Dissolution extraction: pass the dried and fully pulverized Pueraria mirifica stems through a 100-mesh sieve, add NaOH total flavonoids extract with a pH of 10 according to the ratio of solid to liquid ratio of 1:20, extract for 1.5 hours, and then Heated in a microwave oven with a power of 600 watts for 70 seconds, and then the extract was subjected to suction filtration and concentration under reduced pressure.

[0028] 3. Eluting and drying: adjust the treated extract to neutral pH with dilute hydrochloric acid, pass through a 70-mesh polyamide powder column, elute with 85% ethanol...

Embodiment 3

[0031] A method for extracting total flavonoids in Pueraria mirifica stems of the present invention comprises the following steps:

[0032] 1. Material collection: collection of Pueraria mirifica stems with a diameter of 1.5 cm and no pests and diseases for future use. Use the pruning shears to cut the collected kudzu stems into 7cm sections, dry them, and fully crush them for later use.

[0033] 2. Dissolution and extraction: pass the dried and fully pulverized Pueraria mirifica stems through a 100-mesh sieve, add NaOH total flavonoids extract with a pH of 9.5 according to a solid-to-liquid ratio of 1:15, and extract for 3 hours, then Heated in a microwave oven with a power of 500 watts for 75 seconds, and then subjected to suction filtration and vacuum concentration treatment of the extract.

[0034] 3. Eluting and drying: adjust the treated extract to neutral pH with dilute hydrochloric acid, pass through an 80-mesh polyamide powder column, elute with 80% ethanol, freeze a...

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Abstract

The invention relates to a method for extracting total flavones in kudzu stems. The method is characterized by the following extracting steps: a) collecting raw materials; b) dissolving and extracting; and c) eluting and drying. The method disclosed by the invention has the beneficial effects that the technological process is simple and reasonable, the extraction rate of the total flavones of kudzu is increased and the extraction time is shortened; the preparation of the extracting solution is simple, the operation is easy, the cost is low, NaOH in the extracting solution is neutralized by the added diluted hydrochloric acid, the later product safety is higher and the quality is higher; and the kudzu resource is fully utilized, the waste is reduced, the use ratio thereof is increased and the method is worthy of popularization and application.

Description

technical field [0001] The invention relates to a method for extracting total flavonoids in kudzu stems. Background technique [0002] Pueraria mirifica is a perennial vine deciduous plant of the Fabaceae Papilionaceae. It is known as "Shen Nange" and "Asian ginseng". It is a medicinal and edible plant approved by the Ministry of Health. Pueraria mirifica is rich in puerarin, soybean Aglycone and other flavonoids are the main active ingredients in kudzu. Modern medical research shows that flavonoids in kudzu have the functions of treating cardiovascular and cerebrovascular diseases, antithrombotic, improving microcirculation, preventing nervous headaches, reducing Blood sugar and blood lipids, enhance myocardial contractility, enhance immunity and anti-oxidation and other effects. [0003] At present, there are many extraction processes for total flavonoids of Pueraria mirifica, some of which are directly extracted by aqueous solution, but this method is incomplete in mater...

Claims

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Application Information

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IPC IPC(8): A61K36/488A61K135/00
CPCA61K36/488A61K2236/33A61K2236/51A61K2236/55
Inventor 郭坤元林先明郭汉玖穆森王华张宇
Owner 湖北省农业科学院中药材研究所