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High-temperature-resistant deodorizing and nitrogen preserving fermented organic manure strain and preparation method thereof

A technology of fermenting bacterial agent and organic fertilizer, which is applied in the field of preparation of high-temperature resistant deodorizing and nitrogen-preserving organic fertilizer fermenting bacterial agent, can solve problems such as rapid deodorization and nitrogen-retaining deficiency, achieve good ecological and economic benefits, and improve microbial efficiency. Adsorption rate, the effect of improving the production efficiency

Active Publication Date: 2017-10-13
杭州谷胜科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This patent has a very good effect on the degradation and elimination of kitchen waste, but there are still many deficiencies in rapid deodorization and nitrogen retention

Method used

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  • High-temperature-resistant deodorizing and nitrogen preserving fermented organic manure strain and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Carry out the preparation of fermentation bacterial agent according to the following steps:

[0023]1. Perform activation treatment on the slant strains stored in the refrigerator at 4°C, Bacillus subtilis CGMCC1.775, Actinomucor elegans CGMCC3.4389, Bacilus cereus CGMCC1.230, Bacillus licheniformis Seven strains of licheniformis CGMCC1.7461, Rhodopseudomonas CGMCC1.5007, Lactococcus lactisCGMCC1.2281, and Saccharomyces cerevisiae CGMCC2.1643 were cultured in test tube-shaker respectively to obtain first-grade seeds of each strain liquid.

[0024] Bacillus subtilis CGMCC1.775, Bacillus cereusCGMCC1.230 and Rhodopseudomonas CGMCC1.5007 were cultured with beef extract peptone medium: 3g beef extract, 10g peptone, 5g sodium chloride, distilled water 1000mL, adjust the pH value to 7.0; the inoculum size is 1%, 200rpm, and cultivate for 24h.

[0025] Actinomucor elegans CGMCC3.4389 and Saccharomycescerevisiae CGMCC2.1643 were cultured in potato-sucrose medium: take 200 gra...

Embodiment 2

[0033] 1. Activate the seven doses of slant strains stored in the refrigerator at 4°C, Bacillus subtilis CGMCC1.775, Actinomucor elegans CGMCC3.4389, Bacilus cereus CGMCC1.230, Bacillus licheniformis Bacillus licheniformis CGMCC1.7461, Rhodopseudomonas CGMCC1.5007, Lactococcus lactisCGMCC1.2281, and Saccharomyces cerevisiae CGMCC2.1643 were cultured in test tubes and shakers respectively to obtain first-grade seed solutions of each strain.

[0034] Bacillus subtilis CGMCC1.775, Bacillus cereusCGMCC1.230 and Rhodopseudomonas CGMCC1.5007 were cultured with beef extract peptone medium: 3g beef extract, 10g peptone, 5g sodium chloride, distilled water 1000mL, adjust the pH value to 7.2; the inoculum size is 1.5%, 220rpm, culture for 27h.

[0035] Actinomucor elegans CGMCC3.4389 and Saccharomycescerevisiae CGMCC2.1643 were cultivated in potato-sucrose medium: take 200 grams of peeled potatoes, cut them into small pieces, add 1000 mL of water and boil for 30 minutes, filter out pota...

Embodiment 3

[0043] 1. Activate the seven doses of slant strains stored in the refrigerator at 4°C, Bacillus subtilis CGMCC1.775, Actinomucor elegans CGMCC3.4389, Bacilus cereus CGMCC1.230, Bacillus licheniformis Bacillus licheniformis CGMCC1.7461, Rhodopseudomonas CGMCC1.5007, Lactococcus lactisCGMCC1.2281, and Saccharomyces cerevisiae CGMCC2.1643 were cultured in test tubes and shakers respectively to obtain first-grade seed solutions of each strain.

[0044] Bacillus subtilis CGMCC1.775, Bacillus cereusCGMCC1.230 and Rhodopseudomonas CGMCC1.5007 were cultured with beef extract peptone medium: 3g beef extract, 10g peptone, 5g sodium chloride, distilled water 1000mL, adjust the pH value to 7.4; the inoculum size is 2%, 250rpm, and cultivate for 30h.

[0045] Actinomucor elegans CGMCC3.4389 and Saccharomycescerevisiae CGMCC2.1643 were cultivated in potato-sucrose medium: take 200 grams of peeled potatoes, cut them into small pieces, add 1000 mL of water and boil for 30 minutes, filter out ...

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Abstract

The invention relates to a high-temperature-resistant deodorizing and nitrogen preserving fermented organic manure strain and a preparation method thereof. In a secondary high-temperature fermentation and composting process of metabolic biomasses obtained by biological transformation of organic wastes, the strain has the effects of resisting high temperature, removing odor and preserving nitrogen, and is prepared by mixing bacillus subtilis, actinomucor elegans, bacillus cereus, bacillus licheniformis, rhodopseudomonas palustris, lactococcus lactis and saccharomyces cerevisiae. By use of the synergistic effect among organic matter degradation microorganism microbial flora, inhibiting pathogenic bacteria, deodorizing bacteria and high-temperature-resistant acid-forming bacteria, the high-temperature-resistant deodorizing and nitrogen preserving fermented organic manure strain provided by the invention has the characteristics of novelty of a strain combination, stable structure, high activity, high microbial content and the like; meanwhile, the problems of slow organic matter degradation, non-obvious deodorizing effect, low nitrogen content of a composting finished product and the like of conventional organic manure compost and metabolite compost obtained by the biological transformation of the organic wastes are solved; the organic manure treatment efficiency and the nutrient utilization rate are improved.

Description

technical field [0001] The invention belongs to the field of ecological breeding, and relates to a secondary composting treatment technology of metabolized biomass after biologically transforming kitchen waste, in particular to a preparation method of a high-temperature-resistant, deodorizing, nitrogen-conserving organic fertilizer fermentation bacterial agent. Background technique [0002] With the rapid development of my country's social economy and the rapid advancement of modern agriculture, the amount of kitchen waste from urban residents is not only increasing rapidly, but also contains a large amount of water, oil, salt and various nutrients, which are difficult to dispose of through traditional garbage treatment methods. In the research and development process of various decomposition treatments, the biotransformation treatment method gradually shows its advantages. For example, the invention of application number CN201510380599.0 discloses a method for efficiently bi...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C12N1/14C05F9/04C05F11/08C12R1/125C12R1/01C12R1/085C12R1/10C12R1/46C12R1/865
CPCC05F9/04C05F11/08C12N1/14C12N1/18C12N1/20Y02A40/20
Inventor 孙星照张志剑腾昌运冯诗韵
Owner 杭州谷胜科技有限公司
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