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Ratio fluorescence probe for detecting pyrogallic acid as well as preparation method and application

A ratiometric fluorescent probe and pyrogallic acid technology, applied in fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve problems such as unconstructed pyrogallic acid ratiometric fluorescent probes, and achieve operational Effects for simplicity, good selectivity and visualization

Inactive Publication Date: 2017-10-13
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing technology has not yet constructed a ratiometric fluorescent probe for pyrogallic acid with high-precision detection performance.

Method used

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  • Ratio fluorescence probe for detecting pyrogallic acid as well as preparation method and application
  • Ratio fluorescence probe for detecting pyrogallic acid as well as preparation method and application
  • Ratio fluorescence probe for detecting pyrogallic acid as well as preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1) Synthesis of blue fluorescent carbon quantum dots: dissolving citric acid and ammonia water in deionized water to form a precursor solution, subjecting the precursor solution to high temperature treatment, cooling down to room temperature and centrifuging, taking the supernatant for later use;

[0032] 2) Synthesis of silicon oxide nanoparticles embedding carbon quantum dots: Mix cyclohexane, TritonX-100, and n-hexanol, and stir for at least 1 hour to form a microemulsion; take step 1) and disperse the solution in the above microemulsion, and stir for at least 0.5 h Then, add ammonia water and TEOS, stir for 24h, then add APTES, continue to stir for 24h; centrifuge and wash the reaction solution to obtain silicon oxide nanoparticles embedding carbon quantum dots;

[0033] 3) Synthesis of red fluorescent glutathione modified cadmium telluride quantum dots: under nitrogen protection and ice bath conditions, tellurium powder and NaBH 4 Dissolve in deionized water, stir ...

Embodiment 2

[0042] In step 2), when adding step 1) gained solution, also add chitosan solution simultaneously, described step 1) The volume ratio of gained solution and chitosan solution is 5:1; Described chitosan solution is in 1% The mass volume fraction in acetic acid is 0.5%. All the other are consistent with embodiment 1.

experiment example 1

[0044] Pyrogallic acid detection:

[0045] Disperse the probe described in Example 1 in the buffer solution, take multiple sets of reaction test tubes, add appropriate volume of probe solution, pyrogallic acid solution and laccase of known concentration, mix well, and react for a period of time. The reaction solution was added to a quartz dish, and detected by fluorescence spectrophotometry (λex=365nm). Calculate the working curve according to the test results. Such as image 3 As shown, the response range of the probe to pyrogallic acid is 0.05-2 μM, and the linear coefficient of the detection result is 0.993.

[0046] For unknown samples, sample pretreatment is performed first to obtain pyrogallic acid solution of unknown concentration. Take the same volume of probe solution and laccase, add to the sample solution, mix well, and react for a period of time. The reaction solution was added to a quartz dish, and detected by fluorescence spectrophotometry (λex=365nm). Subst...

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Abstract

The invention discloses a ratio fluorescence probe for detecting pyrogallic acid. The visual ratio fluorescence probe is formed by red CdTe quantum dots and blue carbon quantum dots through amido bonds. An experiment finds that a fluorescence quenching phenomenon is gradually enhanced along with the increase of the addition of the pyrogallic acid, and a good linear relationship exists when the linear range is 0.05-2 mu m. An experiment result indicates that a method has the capability of detecting the pyrogallic acid in a water sample simply, conveniently and rapidly.

Description

technical field [0001] The invention belongs to the field of pyrogallic acid detection, in particular to a ratio fluorescent probe for detecting pyrogallic acid and its preparation method and application. Background technique [0002] Pyrogallic acid is a class of phenolic substances that are often used as antioxidants or oxygen scavengers. However, pyrogallic acid has toxicity similar to that of phenol, has a strong irritating effect on skin, eyes, and mucous membranes, and is harmful to aquatic organisms. It may have long-term adverse effects on the water environment, and there may be danger of irreversible consequences. Although existing analytical techniques exhibit good selectivity and sensitivity, they require expensive equipment and complex detection procedures. Therefore, it is necessary to establish a simple detection method to detect pyrogallic acid. [0003] Ratiometric fluorescence analysis provides a visualization and precise analysis of the results. Teng et ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6432
Inventor 王妍媖饶含兵代永成王显祥葛宏伟张兆祎刘鑫刘伟刘亚琴杨燕
Owner SICHUAN AGRI UNIV
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