Sanguinarine/gelatin microspheres-loaded composite hydrogel support and preparation method and application thereof

A technology of compounding hydrogel and sanguinarine, which is applied in the directions of non-active ingredients medical preparations, medical preparations containing active ingredients, and drug combinations, can solve the problems of easy failure of sanguinarine oxidation, and avoid the The effect of drug resistance, wide antibacterial range, and high production efficiency

Inactive Publication Date: 2017-10-17
BEOGENE BIOTECH GUANGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, sanguinarine is prone to failure due to its oxidation, so a new type of drug carrier system must be developed to ensure its efficacy.

Method used

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  • Sanguinarine/gelatin microspheres-loaded composite hydrogel support and preparation method and application thereof
  • Sanguinarine/gelatin microspheres-loaded composite hydrogel support and preparation method and application thereof
  • Sanguinarine/gelatin microspheres-loaded composite hydrogel support and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] A kind of preparation method of the composite hydrogel support of loading sanguinarine / gelatin microspheres of the present embodiment, comprises the following steps:

[0039] (1) Dissolve 100 mg of sodium hyaluronate in 20 ml of deionized water, obtain a 2 mg / ml sodium hyaluronate solution on a shaker at 37 ° C and 100 r / min, and then add 10 times the amount of adipic dihydrazide ( 1g) was dissolved under magnetic stirring at a rate of 400 r / min at room temperature, and the pH after the reaction was adjusted to 6.8 with 0.1 mol / L HCl and 0.1 mol / L NaOH solution. Dissolve 192mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 132mg of 1-hydroxybenzotriazole in 1ml (dimethylsulfoxide:water=1:1) in the mixture. Add the freshly prepared mixed solution dropwise to the reaction solution adjusted to pH=6.8 and continue mixing at room temperature for 6-12 hours, maintaining the pH at 6.8. Use 0.1 mol / L HCl and 0.1 mol / L NaOH solution to adjust the pH after r...

Embodiment 2

[0045] A kind of preparation method of the composite hydrogel support of loading sanguinarine / gelatin microspheres of the present embodiment, comprises the following steps:

[0046] (1) Dissolve 100 mg of sodium hyaluronate in 20 ml of deionized water, obtain a 2 mg / ml sodium hyaluronate solution on a shaker at 37 ° C and 100 r / min, and then add 10 times the amount of adipic dihydrazide ( 1g) was dissolved by magnetic stirring at a rate of 400r / min at room temperature. The pH after the reaction was adjusted to 6.8 with 0.1 mol / L HCl and 0.1 mol / L NaOH solution. Dissolve 192mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 132mg of 1-hydroxybenzotriazole in 1ml (dimethylsulfoxide:water=1:1) in the mixture. Add the freshly prepared mixed solution dropwise to the reaction solution adjusted to pH=6.8 and continue mixing at room temperature for 6-12 hours, maintaining the pH at 6.8. Use 0.1 mol / L HCl and 0.1 mol / L NaOH solution to adjust the pH after reaction...

Embodiment 3

[0052] A kind of preparation method of the composite hydrogel support of loading sanguinarine / gelatin microspheres of the present embodiment, comprises the following steps:

[0053] (1) Dissolve 100 mg of sodium hyaluronate in 20 ml of deionized water, obtain a 2 mg / ml sodium hyaluronate solution on a shaker at 37 ° C and 100 r / min, and then add 10 times the amount of adipic dihydrazide ( 1g) was dissolved by magnetic stirring at a rate of 400r / min at room temperature. The pH after the reaction was adjusted to 6.8 with 0.1 mol / L HCl and 0.1 mol / L NaOH solution. Dissolve 192mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and 132mg of 1-hydroxybenzotriazole in 1ml (dimethylsulfoxide:water=1:1) in the mixture. Add the freshly prepared mixed solution dropwise to the reaction solution adjusted to pH=6.8 and continue mixing at room temperature for 6-12 hours, maintaining the pH at 6.8. Use 0.1 mol / L HCl and 0.1 mol / L NaOH solution to adjust the pH after reaction...

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Abstract

The invention discloses a sanguinarine/gelatin microspheres-loaded composite hydrogel support and a preparation method and an application thereof. The method comprises the following steps: adding a sanguinarine solution dropwisely into gelatin microspheres, placing the materials over night, performing freeze drying to obtain a sanguinarine/gelatin microspheres compound; then adding the compound in an aldehyde glucan-aminated sodium hyaluronate pre-gel solution, uniformly stirring the materials, standing gel at normal temperature, and performing freeze drying to obtain the product. The water absorption of the composite hydrogel support is 23-25 times, the porosity is 68-88%, the aperture is 50-200 [mu]m, the composite hydrogel support has functions of wide-spectrum anti-microbial, long-acting anti-microbial and slow release of medicine, and can be used in the fields of skin wound healing and wound dressing.

Description

technical field [0001] The invention belongs to the technical field of natural polymer materials, in particular to a composite hydrogel scaffold loaded with sanguinarine / gelatin microspheres and its preparation method and application. Background technique [0002] The skin is the largest organ in the human body, accounting for about 16% of the body weight, and is also one of the most vulnerable organs. Skin has thermoregulation, mechanical protection, sensory and body protection functions, and ulceration, trauma, surgical injury and burn are important causes of skin damage. Among them, burns are a world-recognized treatment problem. When there is a lack of proper treatment, skin wounds are often exposed to bacteria that are prone to infection, prolong inflammation, interfere with depigmentation, inhibit collagen production, and delay wound healing. In addition, once bacteria adhere to solid surfaces, they form biofilms that protect the bacteria from the immune system and an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/52A61L27/54A61L27/60A61L27/20A61K9/06A61K47/36A61K47/42A61K31/4741A61P17/02
CPCA61K9/0014A61K9/06A61K31/4741A61K47/36A61K47/42A61L27/20A61L27/52A61L27/54A61L27/60A61L2300/216A61L2300/404A61L2300/60C08L5/02C08L5/08
Inventor 郭瑞蓝咏朱麒宇李丹
Owner BEOGENE BIOTECH GUANGZHOU
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