Compound immobilized enzyme carrier material and preparation method and application thereof

Abstract

The invention discloses a compound immobilized enzyme carrier material and a preparation method and application thereof. The carrier material is prepared as follows: after alkynyl groups are introduced into a reaction of UiO-66-NH2 and 3-propargyl bromide, a click chemistry reaction occurs with poly(tert-butylmethacrylate) of which the end group is nitrine, and then hydrolyzing to obtain nano-particles with the grain diameter of 280 to 320 nm. Through electrostatic incorporation between carboxyl and amino, pectinase is fixed onto the carrier material, so that the active sites capable of being combined with pectinase are also greatly increased while the stability of the pectinase is improved, the adsorbing capacity of the pectinase is high and reaches 89.7% at most; the immobilized pectinase has higher pH, temperature and storage stability and preferable reusability, the relative enzyme activity after eight times of reusability is still 81%, and the carrier material has huge application prospect in the field of immobilized enzymes.

Description

technical field [0001] The invention belongs to the technical field of immobilized enzymes, in particular to a composite immobilized enzyme carrier material and its preparation method and application. Background technique [0002] Immobilized enzymes have been widely used in many fields such as chemistry, medicine, and food because of their better temperature and pH stability, reusability, and easy separation of catalytic products than free enzymes. The choice of immobilized enzyme carrier material is an important factor to determine whether the enzyme can be immobilized successfully and the activity of the immobilized enzyme is high or low. An ideal carrier material should have good mechanical strength, chemical stability, thermal stability, high affinity for enzymes, and be able to maintain high enzyme activity. The carrier materials for immobilized enzymes have developed from the initial natural polymer materials to synthetic polymer materials, inorganic materials, and n...

AI Technical Summary

Problems solved by technology

However, studies have found that although the pore size of most MOFs materials reported so far is adjustable, they are generally microporous structures (the pore size is about 2nm), so the size of the enzyme molecule plays a key role in the loading. Only enzyme molecules with small molecular diameters can be loaded, which limits the application of the interception method

The cross-linking method is to use a cross-linking agent (usually glutaraldehyde) to form a covalent bond with the functional group on the enzyme protein to immobilize the enzyme on the carrier. Although the cross-linking method makes the enzyme and the carrier more effective , the combination is stronger, but the enzyme activity will decrease

[0005] There are also a small number of studies using post-synthesis modification methods to make the surface of MOFs materials have functional groups that can react with enzymes to achieve the purpose of immobilizing enzymes, but the introduced functional groups are not many, resulting in only a small amount of enzymes passing through. Physical adsorption or covalent bonding on the surface of MOFs materials, the immobilization capacity of enzymes has not been greatly improved

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