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Method for promoting expression of grosvenor momordica CYP749A36 gene

A technology of gene expression and Luo Han Guo is applied in the field of plant biology to achieve the effect of increasing content, increasing yield and being environmentally friendly

Inactive Publication Date: 2017-10-20
聂天军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, there is no report on promoting the expression of the CYP749A36 gene in Luo Han Guo to increase the content of sweet glycoside V in Luo Han Guo

Method used

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  • Method for promoting expression of grosvenor momordica CYP749A36 gene

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Effect test

Embodiment 1

[0032] A method for promoting the expression of the CYP749A36 gene of Luo Han Guo comprises inoculating the tissue cultured seedlings of Luo Han Guo in an induction medium for at least 30 days, wherein the concentration of methyl jasmonate in the induction medium is 50 μmol / L.

[0033] Wherein, the induction medium also includes a basal medium, and its formula is: MS+1.5mg / L 6-BA+0.3mg / L IBA+3.5g / L agar+30g / l sucrose+1.0g / L activated carbon .

[0034] Wherein, the induction culture conditions are: relative humidity 60-66%, light intensity 1400 lux, light time 8 h / d, temperature 23±2°C.

[0035] The preparation method of the induction medium is as follows: dissolving methyl jasmonate in 2% ethanol aqueous solution by volume fraction is prepared into a 10000 μmol / L methyl jasmonate mother solution, and sterilized with a 0.22 μm microporous membrane, and the sterilized The final methyl jasmonate mother liquor was added to the basal medium until the concentration of methyl jasmon...

Embodiment 2

[0037]A method for promoting the expression of the CYP749A36 gene of Luo Han Guo, comprising inoculating the tissue cultured plantlets of Luo Han Guo in an induction medium for induction and culturing for at least 30 days, wherein the concentration of methyl jasmonate in the induction medium is 400 μmol / L.

[0038] Wherein, the induction medium also includes a basal medium, and its formula is: MS+1.5mg / L 6-BA+0.3mg / L IBA+3.5g / L agar+30g / l sucrose+1.0g / L activated carbon .

[0039] Wherein, the induction culture conditions are: relative humidity 60-66%, light intensity 1400 lux, light time 8 h / d, temperature 23±2°C.

[0040] The preparation method of the induction medium is as follows: dissolving methyl jasmonate in 2% ethanol aqueous solution by volume fraction to prepare a 10000 μmol / L methyl jasmonate mother solution, and sterilizing it with a 0.22 μm microporous membrane, and sterilizing The final methyl jasmonate mother liquor was added to the basal medium until the conce...

Embodiment 3

[0042] A method for promoting the expression of the CYP749A36 gene of Luo Han Guo, comprising inoculating the tissue cultured seedlings of Luo Han Guo in an induction medium for induction and culturing for 30 days, wherein the concentration of methyl jasmonate in the induction medium is 350 μmol / L.

[0043] Wherein, the induction medium also includes a basal medium, and its formula is: MS+1.5mg / L 6-BA+0.3mg / L IBA+3.5g / L agar+30g / l sucrose+1.0g / L activated carbon .

[0044] Wherein, the induction culture conditions are: relative humidity 60-66%, light intensity 1400 lux, light time 8 h / d, temperature 23±2°C.

[0045] The preparation method of the induction medium is as follows: dissolving methyl jasmonate in 2% ethanol aqueous solution by volume fraction to prepare a 10000 μmol / L methyl jasmonate mother solution, and sterilizing it with a 0.22 μm microporous membrane, and sterilizing The final methyl jasmonate mother liquor was added to the basal medium until the concentration...

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Abstract

The invention discloses a method for promoting expression of a grosvenor momordica CYP749A36 gene. The tissue cultured seedling of the grosvenor momordica is inoculated in an induction medium for induction culture for at least 30 days, wherein, concentration of jasmonic acid methyl ester in the induction medium is 50-400 [mu]mol / L. The method can promote the expression of the grosvenor momordica CYP749A36 gene and accumulation of the mogroside V content.

Description

technical field [0001] The invention relates to the field of plant biotechnology. More specifically, the present invention relates to a method for promoting the expression of Luo Han Guo CYP749A36 gene. Background technique [0002] Mogroside V belongs to the cucurbitane-type tetracyclic triterpenoids. Our research group has deduced the possible biosynthetic pathway of Mogroside V based on the transcriptome data of Luo Han Guo. The precursors for the biosynthesis of mogroside V are isopentenyl diphosphate (IPP) and 3,3-dimethylenylpyrophosphate (DMAPP), both of which are produced by mevalonate (MVA) and methyl Two pathways of erythritol phosphorylation (MEP) are formed, the MVA pathway occurs in the cytoplasm, and the MEP pathway occurs in the plastid. IPP or DMAPP derived from the above two pathways are catalyzed by geranyl pyrophosphate synthase (GPS) to form geranyl pyrophosphate (GPP), and IPP and GPP are catalyzed by farnesyl pyrophosphate synthase (FPS). Then, farne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00A01G7/06C12N5/04
CPCA01H4/008A01G7/06A01H4/001C12N5/0025
Inventor 聂天军韦荣昌
Owner 聂天军
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