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Preparation method for novel separation medium

A separation medium, a new type of technology, applied in the field of preparation of new separation medium, can solve the problems of limited application of mechanical strength, achieve good biological compatibility, easy removal, and excellent mechanical properties

Active Publication Date: 2017-10-20
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the low mechanical strength of conventional hydrogels greatly limits their application in material separation, how to prepare porous hydrogel separation media with good mechanical properties has been a meaningful and challenging problem.

Method used

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  • Preparation method for novel separation medium
  • Preparation method for novel separation medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Preparation of Porous Hydrogel Separation Medium

[0029] Step 1: Dissolve BSA in 10 mL ultrapure water to obtain a BSA solution with a concentration of 100 mg / mL, and add 1% cross-linking agent β-diimine zinc complex and 1,2,7, 1 mL each of 8-dioxoctane, then add 2 mL of 20 mg / mL NaCl, mix well, and adjust the pH of the solution to 2.6 with dilute hydrochloric acid;

[0030] Step 2: Divide the mixed solution in Step 1 into molds, and keep at 40°C for 3 hours to prepare a hydrogel;

[0031] Step 3: Transfer the hydrogel in step 2 into a high-pressure steam sterilizer and keep it at 121°C for 20 minutes, soak and wash it in distilled water for 5 days, and change the washing water every 6 hours to remove salt and residual crosslinking agent;

[0032] Step 4: The washed gel in step 3 was pre-frozen at -80°C for 3 h and then vacuum freeze-dried to obtain a novel porous hydrogel separation medium.

[0033] figure 2 The SEM image of the new hydrogel separation m...

Embodiment 2

[0034] Example 2 Preparation of Porous Hydrogel Separation Medium

[0035] Step 1: Dissolve HSA in 10 mL ultrapure water to obtain a HSA solution with a concentration of 150 mg / mL, and add 1% cross-linking agent β-diimine zinc complex and 1,2,7, 1 mL each of 8-dioxoctane, then add 2 mL of 50 mg / mL KCl, mix well, and adjust the pH of the solution to 3.5 with dilute hydrochloric acid;

[0036] Step 2: Divide the mixed solution in Step 1 into molds, and keep at 50°C for 2 hours to prepare a hydrogel;

[0037] Step 3: Transfer the hydrogel in step 2 into a high-pressure steam sterilizer and keep it at 115°C for 30 minutes, soak and wash it in distilled water for 5 days, and change the washing water every 6 hours to remove salt and residual crosslinking agent;

[0038] Step 4: The washed gel in step 3 was pre-frozen at -80°C for 3 h and then vacuum freeze-dried to obtain a novel porous hydrogel separation medium.

[0039] The physical and chemical properties of the porous hydroso...

Embodiment 3

[0040] Example 3 Preparation of Porous Hydrogel Separation Medium

[0041] Step 1: Dissolve LHC in 10 mL ultrapure water to obtain an LHC solution with a concentration of 100 mg / mL, and add 2% cross-linking agent β-diimine zinc complex and 1,2,7, 1 mL each of 8-dioxoctane, then add 100 mg / mL NaNO 3 Solution 2mL, mix well, and adjust the pH of the solution to 5 with dilute hydrochloric acid;

[0042] Step 2: Divide the mixed solution in Step 1 into molds, and keep it at 60°C for 2 hours to prepare a hydrogel;

[0043] Step 3: Put the primary gel in step 2 into a high-pressure steam sterilizer and keep it at 121°C for 15 minutes, soak and wash in distilled water for 5 days, and change the washing water every 6 hours to remove salt and residual crosslinking agent;

[0044] Step 4: drying the washed gel in step 3 by supercritical carbon dioxide method to obtain a novel porous hydrogel separation medium.

[0045] The physical and chemical properties of the porous hydrosol obtained...

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Abstract

The invention relates to a preparation method for a novel separation medium. The preparation method comprises the following steps: adding a certain quantity of inorganic salt and multiple crosslinking agent beta-diimine zinc compound and 1,2,7,8-diepoxyoctane into macromolecular protein solution; regulating the pH (Potential of Hydrogen) of the solution to be 2-5.5; after even mixing, carrying out water bath heating to prepare saliferous hydrogel; carrying out 110-121 DEG C high-temperature treatment and distilled water dipping and washing on the prepared saliferous hydrogel to remove inorganic salt and monomer crosslinking agent residues; drying to obtain high-intensity porous hydrogel. The hydrogel has the advantages of good elasticity, high resistance to compression and good selective separation ability and is expected to serve as a novel separation medium in a separation purification field, the maximum compressive strain and the maximum compression stress respectively can be 70% and 5-6MPa, and porosity is controllable in 75-90%.

Description

technical field [0001] The invention relates to a preparation method of a novel separation medium, in particular to a preparation method of a novel gel type separation medium. Background technique [0002] Hydrogel is a water-rich polymer with a three-dimensional network structure, which is generally used in the fields of tissue engineering and drug sustained release. Although hydrogels often have controllable porosity, they are often expected to be used for the separation of substances. However, since the low mechanical strength of conventional hydrogels greatly limits their application in material separation, how to prepare porous hydrogel separation media with good mechanical properties has been a meaningful and challenging problem. [0003] The present invention aims to prepare a novel superporous hydrogel. The new hydrogel can overcome the defects of conventional porous hydrogels. Its pore structure is stable, its swelling rate is high, and it has good mechanical prop...

Claims

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Application Information

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IPC IPC(8): C08J3/24C08J3/075C08J9/12B01D17/02B01J20/26B01J20/30C08L89/00
CPCB01D17/0202B01J20/26C08J3/075C08J3/24C08J9/12C08J2389/00
Inventor 惠俊峰范代娣米钰郑晓燕邓建军姜西娟
Owner NORTHWEST UNIV
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