A kind of preparation method of sea cucumber compound soft capsule
A soft capsule and sea cucumber technology, applied in the field of preparation of sea cucumber compound soft capsules, can solve the problems of reduced biological activity and nutrient loss of sea cucumber, and achieve the effects of realizing high-value utilization, promoting development, and strengthening functional activity.
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Embodiment 1
[0027] Remove the viscera of fresh sea cucumbers and homogenate, take 100g of the homogenate, add pH 6.0, 50mM acetic acid buffer to make the substrate concentration reach 15%, and then add 300000U complex protease. In this embodiment, the compound protease is selected from subtilisin complex , product specification: BR, 200U / mg, activity: ≥120units / mg. Enzymolysis was carried out for 3 hours under the microwave-assisted condition of 100W and the reaction temperature of 55°C. The sea cucumber enzymatic hydrolysis solution was inactivated at 95°C for 5 minutes, then concentrated by rotary evaporation at 40°C, freeze-dried, ground into powder, and sieved with a 150-mesh sieve to obtain dry powder of sea cucumber-assisted enzymatic hydrolysis. Then, according to the mass ratio of microwave-assisted enzymatic hydrolysis sea cucumber product dry powder: chitosan oligosaccharide: taurine = 7:1:1 to mix, then add it to distilled water to make the ratio 1:20, and then stir and mix for...
Embodiment 2
[0029] Remove the viscera from fresh sea cucumber and homogenize it, take 100g homogenate, add pH 6.5 50mM acetic acid buffer solution to make the substrate concentration reach 18%, then add 350000U complex protease, and use microwave-assisted conditions of 200W and reaction temperature of 60°C to enzymatically Solution 3.5h. Sea cucumber enzymatic hydrolysis was inactivated at 98°C for 8 minutes, then concentrated by rotary evaporation at 45°C, freeze-dried, ground into powder, and sieved with a 180-mesh sieve to obtain dry powder of sea cucumber-assisted enzymatic hydrolysis. According to the mass ratio, microwave-assisted enzymatic hydrolysis sea cucumber product dry powder: chitosan oligosaccharide: taurine = 8:2:1 is mixed, then added to distilled water to make the ratio 1:25, and then stirred and mixed for spray drying , to obtain a homogeneous microwave-assisted enzymatic hydrolysis sea cucumber compound; finally, the microwave-assisted enzymatic hydrolysis sea cucumber...
Embodiment 3
[0031] Remove the viscera of fresh sea cucumber and homogenize it, take 100g homogenate, add pH 6.5 50mM acetic acid buffer to make the substrate concentration reach 20%, then add 400000U complex protease, under the microwave-assisted condition of 300W, the reaction temperature is 65℃ Solution 4h. The sea cucumber enzymatic hydrolysis solution was inactivated at 100°C for 10 minutes, then concentrated by rotary evaporation at 50°C, freeze-dried, ground into powder, and sieved with a 200-mesh sieve to obtain dry powder of sea cucumber-assisted enzymatic hydrolysis. According to the mass ratio, microwave-assisted enzymolysis sea cucumber product dry powder: chitosan oligosaccharide: taurine = 9:3:2 is mixed, then it is added to distilled water to make the ratio 1:30, and then stirred and mixed for spray drying to prepare A homogeneous microwave-assisted enzymatic hydrolysis sea cucumber compound was obtained; finally, the microwave-assisted enzymatic hydrolysis sea cucumber comp...
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