Magnetic microchip with graphic encoding, its preparation method and application
A graphic coding and microchip technology, applied to microstructure devices without moving components, biochemical equipment and methods, and bioreactors/fermenters for specific purposes, can solve the problems of difficult process and high manufacturing cost. To achieve the effect of expanding the application range, no need for precision equipment, and ensuring accuracy
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[0046] An aspect of the embodiment of the present application provides a method for preparing a magnetic microchip with graphic encoding, including:
[0047] providing a transparent substrate layer;
[0048] Covering the thin film formed by magnetic fluid on the transparent substrate layer;
[0049] removing at least a portion of the base carrier fluid from the thin film, thereby at least partially curing the thin film to form a magnetic fluid layer;
[0050] Processing the magnetic fluid layer to form an opaque microstructure that can be used as a graphic code;
[0051] The opaque microstructure is covered with a transparent material to form the magnetic microchip.
[0052] In some preferred embodiments, the preparation method comprises:
[0053] coating the magnetic fluid on the transparent substrate layer to form the thin film,
[0054] Heating removes at least a portion of the base carrier fluid from the thin film, thereby forming the magnetic fluid layer.
[0055] Am...
Embodiment 1
[0198] Example 1: Surface modification and functionalization of magnetic microchips
[0199] 1) take figure 2 An appropriate amount of a magnetic microchip prepared by the process shown (for example, 50-5 million pieces, depending on specific needs, and the graphic code on each magnetic microchip can also be adjusted according to actual application requirements) is placed in a 1.5mL microcentrifuge tube.
[0200] 2) Wash twice with 95V / V% ethanol. After each wash, enrich the chip to the bottom of the centrifuge tube with a strong magnet or a special magnetic stand for microcentrifuge tubes, and then use a pipette to remove the supernatant.
[0201] 3) Add 1000 μl of 5 wt% aminosilane APDMS (prepared with 95% ethanol), oscillate sufficiently, sonicate for a while, and shake on a vortex shaker for 30 min.
[0202] 4) According to the method described in step 2), wash the chip 3 times with absolute ethanol.
[0203] 5) Wash once with N,N-dimethylformamide (DMF), and remove t...
Embodiment 2
[0214] Example 2 Multiple detection of nucleic acid markers: Taking high-risk human papillomavirus (HPV) as an example
[0215] Human papillomavirus (human papillomavirus HPV) belongs to the papillomavirus genus of the family Papovaviridae. At present, there are more than 200 subtypes of HPV known, which can be divided into 5 genera (alpha, beta, gamma, mu and nu) and 33 species according to their genome similarity. Alpha HPV mainly infects mucosal epithelial cells, among which more than a dozen HPVs are related to cancer, and are called high-risk HPVs. All current clinical HPV typing methods are based on the detection of HPV nucleic acid in clinical samples. HPV typing detection has important guiding significance in early screening of cervical cancer, postoperative follow-up and follow-up, triage of cytological test results, discovery of high-risk groups, and guidance of HPV vaccine research and use. At present, HPV typing detection methods such as membrane reverse hybridiz...
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