Method for preparing carbon quantum dots by using Enteromorpha

A technology for carbon quantum dots and prolifera, which is applied in chemical instruments and methods, nanocarbons, measuring devices, etc., can solve the problems of cumbersome process steps, high equipment requirements, wide particle size range, etc., and achieves simple preparation process operation, fluorescence The effect of excellent properties and low preparation cost

Inactive Publication Date: 2017-11-03
QINGDAO UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the raw materials in this preparation method are cheap and easy to obtain, the process steps are cumbersome, require high equipment, and take a long time, which is not conducive to large-scale production; the Chinese invention patent with application number 201210264411.2 discloses a kind of bean dregs after squeezing soybean milk from soybeans The preparation method of fluorescent carbon quantum dots as raw materials mainly includes the following steps: drying and dehydrating the raw materials to obtain bean dregs powder; heating at 200°C for 20-40min to carbonize; grinding and sieving to obtain coarse powder; The solution was freeze-dried at -50°C to obtain fluorescent carbon quantum dots
Although the process of this method is relatively simple, it requires complex pretreatment of raw materials, which greatly increases the production cost
In addition, the particle size uniformity of the carbon quantum dots prepared in the examples is poor, and the particle size range is wide, about 3-12 μm

Method used

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  • Method for preparing carbon quantum dots by using Enteromorpha
  • Method for preparing carbon quantum dots by using Enteromorpha
  • Method for preparing carbon quantum dots by using Enteromorpha

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Weigh 2g of the collected Enteromorpha, rinse it with deionized water, put it into a 150ml polytetrafluoroethylene reactor, and then add 120ml of pH 10.0 borate buffer solution.

[0026] (2) Put the reactor into a vacuum drying oven and react at 180° C. for 6 hours, then take out the reactor and cool to room temperature to obtain a carbon quantum dot solution.

[0027] (3) The carbon quantum dot solution prepared by step (2) is centrifuged at 11900rpm for 20min to remove solid impurities and precipitates, and the supernatant is the prepared carbon quantum dot.

Embodiment 2

[0029] In this embodiment, the carbon quantum dot solution prepared in Example 1 is applied to Fe 3+ For the detection of characteristic ions, add 150μM Ag + ,Al 3+ ,Cd 2+ ,Cu 2+ ,Co 2+ , Fe 2+ , Fe 3+ ,Hg 2+ ,Mg 2+ ,Mn 2+ , Ni 2+ ,Pb 2+ ,Zn 2+ , and measured its fluorescence intensity at an excitation wavelength of 360nm, in which only Fe 3+It has an obvious quenching effect on the fluorescence intensity of carbon quantum dots, and the addition of other different metal ions will not have a significant impact on the fluorescence intensity of carbon quantum dots. In order to explore the added Fe 3+ The linear relationship between concentration and fluorescence intensity, prepare a series of concentration gradient Fe 3+ , 1 μM, 10 μM, 50 μM, 100 μM, 120 μM, 150 μM, 170 μM, 200 μM, 220 μM, 250 μM, 270 μM, 300 μM, 320 μM, 350 μM, 370 μM, 400 μM, 500 μM, 700 μM, 1000 μM, 1200 μM, 1500 μM were added to the same Concentration of carbon quantum dot solution, get a serie...

Embodiment 3

[0031] In this embodiment, the carbon quantum dot solution prepared in Example 1 is applied to cell imaging, and HeLa cells (10 6 cells / sample) placed on a square glass slide with a side length of 35mm, prepare a fresh DMEM solution containing carbon quantum dot solution concentration 1 / 10, and incubate HeLa cells with the prepared DMEM solution at 37°C for 4-24h to obtain incubated cells; At room temperature, all the incubated cells were washed three times with phosphate buffer solution to remove excess unabsorbed carbon quantum dots, the 488nm Ar ion laser was selected to excite the incubated cells, and the incubated cells were collected by a LEICA TCS SP2 laser scanning confocal microscope Obtain images, and further collect cell imaging conditions in a fixed area over time, such as Figure 7 As shown, visible cells imaged very well.

[0032] In this example, the cytotoxicity is tested, and the cells are incubated with DMEM solutions containing different concentrations of c...

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Abstract

The invention belongs to the technical field of preparation of carbon nanometer materials and new marine materials, and relates to a method for preparing carbon quantum dots by using Enteromorpha in sea, and applications of the prepared carbon quantum dots in metal ion detection, biological imaging and other fields. The method comprises: rinsing enteromorpha by using deionized water, placing into a 150 ml polytetrafluoroethylene reaction kettle, adding a borate buffer solution with the pH value of 10.0, placing the reaction kettle into a vacuum oven, carrying out a reaction for 6 h at a temperature of 180 DEG C, taking out the reaction kettle, cooling to a room temperature to obtain a carbon quantum dot solution, carrying out centrifugation on the obtained carbon quantum dot solution for 20 min through a centrifuge under a condition of 11900 rpm, removing solid impurities and precipitate, and taking the supernatant so as to obtain the carbon quantum dots. According to the present invention, the method has characteristics of simple preparation process, convenient operation, easily available raw materials and low preparation cost, and the prepared carbon quantum dots have advantages of no toxicity, uniform particle size, excellent fluorescence property, and good stability.

Description

Technical field: [0001] The invention belongs to the technical field of preparation of carbon nanomaterials and marine new materials, and relates to a process for preparing carbon quantum dots by a hydrothermal method, in particular to a method for preparing carbon quantum dots by using Enteromorpha in the ocean, and preparing the prepared carbon quantum dots It can be applied to the fields of metal ion detection and biological imaging. Background technique: [0002] Due to global climate change, water eutrophication and other reasons, a large number of Enteromorpha green tides broke out in the ocean. A large number of Enteromorpha can block sunlight and affect the growth of algae on the seabed; dead Enteromorpha can also consume oxygen in seawater, and a large number of Enteromorpha floats and gathers on the shore, blocking waterways, destroying marine ecosystems, and seriously threatening coastal fisheries , Tourism development. Therefore, the recycling and rational util...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B32/15B82Y40/00C09K11/65G01N21/64
CPCG01N21/6428C01P2002/72C01P2002/82C01P2002/84C01P2002/85C01P2004/02C01P2004/04C01P2004/52C01P2006/60C09K11/65G01N2021/6432
Inventor 许元红刘敬权刘梦丽牛富双
Owner QINGDAO UNIV
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