Strain preservation method of anaerobic methanogens in coal seams

A technology for anaerobic methanogenic bacteria and bacterial species preservation, which is applied in the field of bacterial species preservation of coal seam anaerobic methanogenic bacteria groups, can solve the problem that the bacterial species preservation method cannot meet the short-term and long-term preservation of bacterial species, and is beneficial to growth and reproduction. , high active effect

Active Publication Date: 2017-11-03
SHANXI JINCHENG ANTHRACITE COAL MINING GRP CO LTD
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Problems solved by technology

[0005] The present invention provides a coal seam anaerobic methanogenic bacteria group in order to solve the problem that the existing strain preservation method cannot satisfy the short-term ...

Method used

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  • Strain preservation method of anaerobic methanogens in coal seams

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Embodiment 1

[0016] Embodiment 1: a kind of strain preservation method of coal seam anaerobic methanogenic flora, comprises the steps:

[0017] (1) Screening of bacterial strains to be preserved: According to the previous experimental experience of the State Key Laboratory of Coal and Coalbed Methane Co-mining in Shanxi Jincheng Anthracite Mining Group Co., Ltd., coal seam produced water was collected at the outlet of coalbed methane production wells, and the produced water was tested. Methanogenic bacteria are enriched and domesticated, and the culture medium of bacteria whose methane production reaches more than 30% is used as the bacteria solution to be preserved;

[0018] (2) Short-term preservation: add the bacteria liquid to be preserved obtained in step (1) into a 5ml anaerobic tube, then add an equal volume of sterilized glycerol / deoxygenated L-cysteine ​​solution, glycerol / L-cysteine In the cystine solution: the mass fraction of glycerol is 30%, and the final concentration of L-cy...

Embodiment 2

[0020] Example 2: The storage method is the same as that described in Example 1, and it is stored at 4 ° C. After 15-30 days, it is directly used as an inoculant for biogas production experiments, and the results are observed.

Embodiment 3

[0021] Example 3: The storage method is the same as that described in Example 1, and it is stored at 25°C. After 15-30 days, it is directly used as an inoculant for biogas production experiments, and the results are observed.

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Abstract

The invention belongs to the technical field of microbial engineering. In order to solve the problems that the existing strain preservation method cannot meet the requirements of short-term and long-term preservation of strains of methanogens produced by coal-bed methane and cannot maintain the activity of high-yield methane gas of the strains, the invention provides a strain preservation method of anaerobic methanogens in coal seams. The method comprises the steps of screening a strain to be preserved, adding the screened strain into a 5ml of anaerobic tube, then adding an equivoluminal sterilized glycerin/deoxidized L-cysteine solution, evenly mixing the mixed solution, and then preserving; long-term preservation: mixing lump anthracite coal, a preservation nutrient solution, L-cysteine, resazurin and a bacterium solution according to a certain ratio and then preserving the mixture at room temperature, simulating the environmental conditions of the coal seams, and enabling the methanogens to maintain higher activity and a more stable bacterial flora structure all the time so as to obtain more ideal methane yield. The strain preservation method does not need ultra-low temperature equipment and the like, does not need special equipment, is convenient and fast, is suitable for short-term and long-term preservation of different amounts of strains, is economical and convenient, and lowers the production and test costs.

Description

technical field [0001] The invention belongs to the technical field of microbial engineering, and in particular relates to a strain preservation method of coal seam anaerobic methanogenic flora. Background technique [0002] As the basic material for microbiological research, bacterial strains are important resources of the country. Especially for the use of microorganisms to carry out related production, it is inseparable from strains. Therefore, strain preservation is essential in microbiology research and practical application. [0003] At present, the methods of bacterial strain preservation mainly include: glycerol cryopreservation method, liquid paraffin preservation method, vacuum freeze-drying preservation method, cryopreservation method and carrier preservation method, etc. The production of biological coalbed methane is produced by the joint action of various anaerobic mixed bacteria to metabolize the coal matrix, so the relative stability of the structure of the...

Claims

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Application Information

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IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 韩作颖陈彦梅魏国琴赵晗杨秀清
Owner SHANXI JINCHENG ANTHRACITE COAL MINING GRP CO LTD
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