Method for culturing bacillus subtilis fungicide and composite fertilizer containing fungicide
A technology of Bacillus subtilis and bacteria agent, which is applied in the field of compound fertilizer and its preparation, can solve the problems of impact and low microbial degradation efficiency, and achieve the effects of improving disease resistance, saving money, and reducing dependence on chemical fertilizers
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Embodiment 1
[0030] The compound fertilizer containing Bacillus subtilis bacteria agent is composed of the following raw materials in parts by weight: humus 50kg, silt 40kg, chicken manure 35kg, straw 30kg, urea 20kg, superphosphate 20kg, potassium chloride 20kg, water 15kg, Bacillus subtilis 5kg, Bacillus megaterium 5kg, Rhodococcus powder 5kg, Bacillus 1.2kg, Yeast 1.2kg, Lactic acid bacteria 1.2kg.
[0031] In the present embodiment, the preparation process of the bacillus subtilis bacterial agent is as follows:
[0032] (1) Using a sterile pipette, draw 0.3-0.5mL sterile normal saline, drop it into the strain preservation tube, and shake it slightly until it is evenly suspended, draw 0.2mL of the bacteria solution into the beef extract peptone liquid medium, shake at 35°C Cultivate for 1-2 days to obtain the bacterial liquid;
[0033] (2) Move the bacterium solution obtained in step (1) to the beef broth peptone medium, place the beef broth peptone medium in a thermostat at 35° C. and...
Embodiment 2
[0045] The compound fertilizer containing Bacillus subtilis bacteria agent is composed of the following raw materials in parts by weight: humus 60kg, sludge 45kg, chicken manure 40kg, straw 35kg, urea 25kg, superphosphate 25kg, potassium chloride 25kg, water 16kg, Bacillus subtilis 6kg, Bacillus megaterium 6kg, Rhodococcus powder 6kg, Bacillus 2.2kg, Yeast 2.2kg, Lactic acid bacteria 2.2kg.
[0046] In the present embodiment, the preparation process of the bacillus subtilis bacterial agent is as follows:
[0047] (1) Using a sterile pipette, draw 0.3-0.5mL sterile normal saline, drop it into the strain preservation tube, and shake it slightly until it is evenly suspended, draw 0.2mL of the bacteria solution into the beef extract peptone liquid medium, shake at 35°C Cultivate for 1-2 days to obtain the bacterial liquid;
[0048] (2) move the bacterium solution obtained in step (1) to the beef broth peptone medium, place the beef broth peptone medium in a thermostat at 35° C. a...
Embodiment 3
[0060] The compound fertilizer containing Bacillus subtilis bacteria agent is composed of the following raw materials in parts by weight: humus 70kg, silt 45kg, chicken manure 40kg, straw 35kg, urea 25kg, superphosphate 25kg, potassium chloride 25kg, water 18kg, Bacillus subtilis 8kg, Bacillus megaterium 8kg, Rhodococcus powder 8kg, Bacillus 3kg, Yeast 3kg, Lactic acid bacteria 3kg.
[0061] In the present embodiment, the preparation process of the bacillus subtilis bacterial agent is as follows:
[0062] (1) Using a sterile pipette, draw 0.3-0.5mL sterile normal saline, drop it into the strain preservation tube, and shake it slightly until it is evenly suspended, draw 0.2mL of the bacteria solution into the beef extract peptone liquid medium, shake at 35°C Cultivate for 1-2 days to obtain the bacterial liquid;
[0063] (2) move the bacterium solution obtained in step (1) to the beef broth peptone medium, place the beef broth peptone medium in a thermostat at 35° C. and culti...
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