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Method for culturing bacillus subtilis fungicide and composite fertilizer containing fungicide

A technology of Bacillus subtilis and bacteria agent, which is applied in the field of compound fertilizer and its preparation, can solve the problems of impact and low microbial degradation efficiency, and achieve the effects of improving disease resistance, saving money, and reducing dependence on chemical fertilizers

Inactive Publication Date: 2017-11-07
JIANGSU FOOD & PHARMA SCI COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Netherlands, Japan, Germany, and the United States have successively invested a large amount of funds in the research and application of soil pollution recovery, but the use of microorganisms for bioremediation is often affected due to its low degradation efficiency. Therefore, a new type of microbial fertilizer is urgently needed. Realize automatic remediation of soil pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The compound fertilizer containing Bacillus subtilis bacteria agent is composed of the following raw materials in parts by weight: humus 50kg, silt 40kg, chicken manure 35kg, straw 30kg, urea 20kg, superphosphate 20kg, potassium chloride 20kg, water 15kg, Bacillus subtilis 5kg, Bacillus megaterium 5kg, Rhodococcus powder 5kg, Bacillus 1.2kg, Yeast 1.2kg, Lactic acid bacteria 1.2kg.

[0031] In the present embodiment, the preparation process of the bacillus subtilis bacterial agent is as follows:

[0032] (1) Using a sterile pipette, draw 0.3-0.5mL sterile normal saline, drop it into the strain preservation tube, and shake it slightly until it is evenly suspended, draw 0.2mL of the bacteria solution into the beef extract peptone liquid medium, shake at 35°C Cultivate for 1-2 days to obtain the bacterial liquid;

[0033] (2) Move the bacterium solution obtained in step (1) to the beef broth peptone medium, place the beef broth peptone medium in a thermostat at 35° C. and...

Embodiment 2

[0045] The compound fertilizer containing Bacillus subtilis bacteria agent is composed of the following raw materials in parts by weight: humus 60kg, sludge 45kg, chicken manure 40kg, straw 35kg, urea 25kg, superphosphate 25kg, potassium chloride 25kg, water 16kg, Bacillus subtilis 6kg, Bacillus megaterium 6kg, Rhodococcus powder 6kg, Bacillus 2.2kg, Yeast 2.2kg, Lactic acid bacteria 2.2kg.

[0046] In the present embodiment, the preparation process of the bacillus subtilis bacterial agent is as follows:

[0047] (1) Using a sterile pipette, draw 0.3-0.5mL sterile normal saline, drop it into the strain preservation tube, and shake it slightly until it is evenly suspended, draw 0.2mL of the bacteria solution into the beef extract peptone liquid medium, shake at 35°C Cultivate for 1-2 days to obtain the bacterial liquid;

[0048] (2) move the bacterium solution obtained in step (1) to the beef broth peptone medium, place the beef broth peptone medium in a thermostat at 35° C. a...

Embodiment 3

[0060] The compound fertilizer containing Bacillus subtilis bacteria agent is composed of the following raw materials in parts by weight: humus 70kg, silt 45kg, chicken manure 40kg, straw 35kg, urea 25kg, superphosphate 25kg, potassium chloride 25kg, water 18kg, Bacillus subtilis 8kg, Bacillus megaterium 8kg, Rhodococcus powder 8kg, Bacillus 3kg, Yeast 3kg, Lactic acid bacteria 3kg.

[0061] In the present embodiment, the preparation process of the bacillus subtilis bacterial agent is as follows:

[0062] (1) Using a sterile pipette, draw 0.3-0.5mL sterile normal saline, drop it into the strain preservation tube, and shake it slightly until it is evenly suspended, draw 0.2mL of the bacteria solution into the beef extract peptone liquid medium, shake at 35°C Cultivate for 1-2 days to obtain the bacterial liquid;

[0063] (2) move the bacterium solution obtained in step (1) to the beef broth peptone medium, place the beef broth peptone medium in a thermostat at 35° C. and culti...

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Abstract

The invention discloses a composite fertilizer containing a bacillus subtilis fungicide and a preparation method of the composite fertilizer and belongs to the technical field of fertilizers. The composite fertilizer is composed of the following raw materials in parts by weight: 50-80 parts of humus, 40-60 parts of sludge, 35-50 parts of chicken manure, 30-40 parts of straws, 20-30 parts of urea, 20-30 parts of calcium superphosphate, 20-30 parts of potassium chloride, 15-20 parts of water, 5-10 parts of bacillus subtilis fungicide, 5-10 parts of bacillus megatherium, 5-10 parts of rhodococcus rhodococcus powder and the balance of EM bacteria. The novel composite microbial fertilizer disclosed by the invention has the effects of improving physical and chemical properties of soil, improving the nutrient supplying capability of soil, reducing dependence of crops on the chemical fertilizer and inhibiting plant diseases and insect pests. Therefore, the novel composite microbial fertilizer and the preparation method thereof are suitable to be popularized and used in the field of fertilizers.

Description

technical field [0001] The invention relates to the technical field of fertilizers, in particular to a compound fertilizer containing Bacillus subtilis bacterial agent and a preparation method thereof. Background technique [0002] With the rise of biotechnology in the 21st century, the development and application of bio-fertilizers will create a good rhizosphere ecological environment, improve soil physical and chemical properties, improve soil fertilizer supply capacity, reduce crop dependence on chemical fertilizers, inhibit the occurrence of pests and diseases, and reduce pesticides. Pollution etc. play an important role. This means that not only the cost of agricultural production may be greatly reduced, but it will also have a profound impact on the sustainable development of agriculture. At the same time, it is also of great significance to the recycling of resources and the integration of ecological, economic and social benefits. The irrational use of pesticides has...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05G3/00C05G3/02C05G3/04C12R1/125C05G3/60C05G3/80
CPCC05B1/02C05G3/00C05G3/60C05G3/80C12N1/20C05C9/00C05D1/02C05F3/00C05F7/00C05F11/00C05F11/02C05F11/08
Inventor 杨猛范广璞许楠楠
Owner JIANGSU FOOD & PHARMA SCI COLLEGE
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