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Method for producing acetic acid from corn straw through anaerobic fermentation

A technology of anaerobic fermentation and corn stalks, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problem of unsatisfactory effect of compound flora of artificial components, poor treatment effect of single microorganism, and pretreatment time Long-term problems, to achieve important industrial application value and development prospects, high-efficiency lignocellulose degradation ability, and low equipment requirements

Active Publication Date: 2017-11-07
GANSU ACAD OF SCI INST OF BIOLOGY
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AI Technical Summary

Problems solved by technology

The common pretreatment methods of lignocellulose include mechanical method, heat treatment method and chemical treatment, which can effectively promote anaerobic digestion, but these pretreatment methods are costly and not environmentally friendly
Ordinary microbial treatment also has many defects. The effect of single microbial treatment is not good, and the effect of composite flora of artificial components is not ideal. There is mutual antagonism among various strains, resulting in long pretreatment time and low conversion efficiency. There is a well-established protocol for the anaerobic fermentation of corn stover to produce acetic acid
[0004] It is a newer and more effective method to treat corn stalks with a naturally coexisting complex microbial system. The production of acetic acid has achieved certain results. Among them, corn stalks are used as substrate for anaerobic fermentation, and the highest yield of acetic acid is 41.96mM. Corn cob powder is used as substrate for anaerobic fermentation. The yield of acetic acid is 40mM, and the yield of acetic acid is relatively low. , the existing technology needs to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment one, Piromyces Yak TZ +M. ruminantium Preparation of bacteria.

[0029] Draw 1mL Piromyces Yak TZ +M. ruminantium The co-culture was inoculated into 9 mL of anaerobic medium based on air-dried and pulverized wheat straw in a 20 mL Heinz anaerobic tube, and compound antibiotics were added at the same time to make the final concentration of the anaerobic medium solution be Penicillin 1600 IU / mL and streptomycin sulfate 2000 IU / mL. After anaerobic culture at 39°C for 72 hours, the growth peak is reached, and the fermentation broth is a high-activity bacterial agent at this time.

Embodiment 2

[0030] Embodiment 2, anaerobic fermentation of corn stalks to produce acetic acid.

[0031] 45 mL of liquid basic medium was placed in a 100 mL volume anaerobic fermentation bottle, and 0.5 g of crushed and air-dried corn stover was used as the substrate. Deoxygenation. Sterilize. Subcultured for 72 h Piromyces Yak TZ +M. ruminantium Use a sterile syringe to draw 5 mL of the co-culture and inoculate it into the above-mentioned anaerobic medium with corn stalks. At the same time, add compound antibiotics so that the final concentration in the anaerobic medium solution is 1600 IU / mL of penicillin and streptomycin sulfate. 2000 IU / mL, cultured anaerobically at 39 °C for 7 days. A total of 3 parallel experiments were set up, and the amount of acetic acid in the fermentation broth was measured every 24 h.

[0032] The measurement method is: the co-cultivation fermentation liquid is at 4 ℃, 10000× g After centrifugation for 10 min, the supernatant was taken to measure acetic ...

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Abstract

The invention discloses a method for producing acetic acid from corn straw through anaerobic fermentation. The fermentation bacterial agent adopted in the method is naturally coexisting anaerobic fungi and PiromycesYak Tz+M. ruminantium, which are separated from the rumen liquid of yaks which are fully grazed in the Nanniwan pasture located in Tianzhu, Gansu in the Qinghai-Tibet plateau. The coculture is deposited with the China General Microbiological Culture Collection Center and assigned the accession number CGMCC No. 12952. The coculture is taken as a bacterial agent for anaerobic fermentation of corn straw, acetic acid can be efficiently produced, and in 7 days of a culture period, the coculture can degrade corn straw to generate 49.0 mM of acetic acid. The method is short in fermentation time and simple in fermentation process, and has bright application development prospects.

Description

technical field [0001] The invention relates to the field of biotechnology renewable energy, in particular to a method for anaerobically fermenting corn stalks to produce acetic acid. Background technique [0002] Corn is the main food crop in my country, with a wide sowing area, and the amount of straw produced every year is also very huge. At present, a large number of corn stalk resources in rural areas of our country are completely in the state of high consumption, high pollution, low utilization rate and low output, and corn stalks have not been rationally developed and utilized as an energy source. Corn stalks can be regenerated as resources through anaerobic digestion treatment, but the existing anaerobic digestion technology has the problems of low technical efficiency and great difficulty in popularization. [0003] Lignocellulose is the main component of corn stover, and the hydrolysis of lignocellulose is the rate-limiting step in the whole anaerobic digestion, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P7/54C12R1/01C12R1/645
CPCC12P7/54C12P39/00
Inventor 魏亚琴周剑平王治业
Owner GANSU ACAD OF SCI INST OF BIOLOGY
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