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Method for preparing rodlike fluorescent labeled microplastics

A technology of fluorescent labeling and microplastics, applied in the preparation of test samples, analysis of materials, sampling, etc., can solve the problem that plastic microspheres with uniform shape cannot simulate microplastics well, and achieve uniform shape and simple operation Effect

Inactive Publication Date: 2017-11-07
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Purpose of the invention: The technical problem to be solved by the present invention is aimed at the deficiencies of the prior art. At this stage, the use of plastic microspheres with uniform shape in the research on the toxicity of microplastics cannot well simulate the current situation of microplastics of different shapes in the environment. A method for preparing rod-shaped fluorescently labeled microplastics, prepared by swelling adsorption method and frozen section method

Method used

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  • Method for preparing rodlike fluorescent labeled microplastics
  • Method for preparing rodlike fluorescent labeled microplastics
  • Method for preparing rodlike fluorescent labeled microplastics

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Preparation of rod-shaped polystyrene fluorescently labeled microplastics:

[0036] (1) Dissolve Nile Red dye in acetone to prepare a 2.5 g / L stock solution of Nile Red dye. Use a 5 mL centrifuge tube to mix the Nile red dye stock solution with n-hexane at a volume ratio of 1:4. Add unlabeled polystyrene fibers into the mixture, vortex and let stand for 24 h, use a pipette gun to carefully absorb the mixture, and wash three times with ultrapure water to obtain fluorescently labeled polystyrene fibers.

[0037] (2) Fluorescently labeled polystyrene fibers are wound on custom-made spools (such as figure 2 shown) come up to align the fibers and the ends are taped as image 3 .

[0038] (3) Coat the aligned fibers with frozen embedding agent and freeze them in a -80°C refrigerator for 26 hours to fix and make the fibers brittle. Use a knife to cut the frozen block into 2cm-long parallel pieces, and arrange 8 of them neatly to make a standard 3.5cm×1cm×2cm cube, wrap it...

Embodiment 2

[0042] Preparation of rod-shaped polypropylene fluorescently labeled microplastics:

[0043] (1) Dissolve Nile Red dye in acetone to prepare a 3 g / L stock solution of Nile Red dye. Use a 5 mL centrifuge tube to mix the Nile red dye stock solution with n-hexane at a volume ratio of 1:3. Add unlabeled polypropylene fibers into the mixture, vortex and let stand for 36 h, use a pipette gun to carefully absorb the mixture, and wash 4 times with ultrapure water to obtain fluorescently labeled polypropylene fibers.

[0044] (2) Fluorescently labeled polypropylene fibers are wound on custom-made spools (such as figure 2 shown) up to align the fibers and the ends are secured with tape.

[0045] (3) Coat the aligned fibers with frozen embedding agent and freeze them in a -60°C refrigerator for 26 hours to fix and make the fibers brittle. Use a knife to cut the frozen block into 2cm-long parallel pieces, and arrange 9 of them neatly to make a standard 3.5cm×1cm×2cm cube, wrap it in t...

Embodiment 3

[0049] Preparation of rod-shaped polyethylene fluorescently labeled microplastics:

[0050] (1) Dissolve Nile Red dye in acetone to prepare a 5 g / L stock solution of Nile Red dye. Use a 5 mL centrifuge tube to mix the Nile red dye stock solution with n-hexane at a volume ratio of 1:2. Add unlabeled polyethylene fibers into the mixture, vortex and let stand for 48 h, use a pipette gun to carefully absorb the mixture, and wash 5 times with ultrapure water to obtain fluorescently labeled polyethylene fibers.

[0051] (2) Fluorescently labeled polyethylene fibers are wound on custom-made spools (such as figure 2 shown) up to align the fibers and the ends are secured with tape.

[0052] (3) Coat the aligned fibers with frozen embedding agent and freeze them in a -40°C refrigerator for 26 hours to fix and make the fibers brittle. Use a knife to cut the frozen block into 2cm-long parallel pieces, and arrange 10 of them neatly to make a standard 3.5cm×1cm×2cm cube, wrap it in tin fo...

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Abstract

The invention discloses a method for preparing rodlike fluorescent labeled microplastics. The method comprises the steps of adding plastic fibers to a mixed solution of a dye stock solution and n-hexane for fluorescent labeling, winding the plastic fibers on a reel to align the plastic fibers and coating the plastic fibers with a frozen section medium; freezing, cutting into blocks, arranging the blocks into a cube, and fixing the cube on a histotome for continuous slicing; and finally washing slices by using ultrapure water, drying and weighing. Through the method, the common fluorescent microplastics of different shapes in the environment can be conveniently and quickly obtained, qualitative and quantitative analysis of distribution and enrichment rules of the microplastics of different shapes in an environment medium and a living body is facilitated, and the method can be widely applied to ecological environment risk research of various microplastics.

Description

technical field [0001] The invention relates to a method for preparing rod-shaped fluorescently labeled microplastics. The prepared microplastics are used to qualitatively and quantitatively analyze the distribution and enrichment rules of microplastics of different shapes in environmental media and organisms, and can be widely used in various types of microplastics. The ecological environment risk research belongs to the field of polymer material preparation. Background technique [0002] Microplastics refer to plastic particles with a diameter of less than 5mm. Plastic pollution has become a major problem in the ocean due to the increasing production of plastics and the fact that they are difficult to degrade. Ultraviolet radiation and mechanical abrasion can degrade plastics into small-sized microplastics, and the potential environmental health risks of microplastics have been paid more and more attention. Literature research found that in the actual environment and bio...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/36G01N1/42G01N1/34
CPCG01N1/28G01N1/286G01N1/34G01N1/36G01N1/42G01N2001/2873
Inventor 张宴乔如霞任洪强
Owner NANJING UNIV
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