A method for preparing an antioxidant diluent for low-temperature storage of pig seminal fluid

A technology for cryopreservation and pig semen, which is applied in the preservation, application, animal husbandry and other directions of human or animal body, and can solve the problems of semen pollution and poor effect.

Inactive Publication Date: 2017-11-10
安徽泗县成安牧业科技有限公司
View PDF0 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For pig sperm, weak acid and slightly higher osmotic pressure are more conducive to its low-temperature storage at 4 °C. Artificially collected semen is prone to microbial contamination, and penicillin and streptomycin are widely used as antibacterial agents in diluents at room temperature. Inhibit the growth and reproduction of microorganisms in preservation diluents, but the two are less effective against Gram-negative bacteria such as Escherichia coli

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) First of all, beer yeast mud is used as the source of yeast, washed with water for 3 to 5 times, and then centrifuged to obtain a yeast liquid containing yeast cells, and the effective number of viable cells of beer yeast is not less than 1.0×10 6 cfu / ml.

[0021] (2) Broken the yeast cells by the action of microwave radiation. Under the electromagnetic environment with a microwave power of 700W for 4 minutes, grind the grape seeds through a 100-mesh sieve into a fine powder. Organic solvent ethanol, control ethanol concentration 55%, adjust the power of microwave radiation to 300W, act for 45min, belong to the extraction process.

[0022] (3) Add a certain amount of biological flocculant sodium carboxymethyl cellulose to the extract described in (2), control the concentration of the diluent to 15~20g / L, keep it in a constant temperature water bath for 20min, and then perform centrifugation, and take For the supernatant, ethanol is recovered by distillation under r...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for preparing an antioxidant diluent for low-temperature preservation of pig semen. The method comprises the following steps: obtaining yeast cell liquid, then crushing yeast cells, adding an organic solvent for extraction, extracting the supernatant and concentrating , made after adding excipients at last; by extracting yeast from waste beer yeast mud, breaking the yeast, using the yeast extract as the bottom liquid, and adding other active ingredients, a dilution of pig semen for low-temperature storage is made, and the release is controlled at the same time The power of the microwave and the concentration of added ethanol not only extracted a certain amount of yeast trehalose, but also successfully extracted a certain amount of proanthocyanidins from the added grape seed powder, which proved to be very good in the frozen semen dilution. Oxidizing substances, effective freezing diluents for porcine semen preservation.

Description

technical field [0001] The invention belongs to the technical field of breeding industry, and in particular relates to a method for preparing an antioxidant diluent for low-temperature preservation of pig semen. Background technique [0002] Semen preservation is the key to pig artificial insemination technology. It can not only prolong the storage time of sperm, but also facilitate long-distance transportation. Compared with cryopreservation, pig semen cryopreservation technology is convenient and economical in terms of transportation and operation, and the storage time is longer than normal temperature storage, and the cost is lower. At present, research on cryopreservation is still in the stage of screening of diluents. [0003] Cryoprotection is an important factor affecting the quality of pig frozen semen. Since people discovered the cryoprotective properties of glycerol, it has rapidly promoted the development of livestock frozen semen. However, glycerol, as a permeab...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 邓衍安陈军辉
Owner 安徽泗县成安牧业科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap