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Method for extracting high-purity total alkaloids from justicia adhatoda

A technology of total alkaloids and duckbill flowers, applied in the direction of pharmaceutical formulas, plant raw materials, medical preparations containing active ingredients, etc., to achieve the effects of reducing the possibility of environmental waste emissions, fully utilizing resources, and reducing production costs

Inactive Publication Date: 2017-11-24
SUZHOU UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there are few studies on extracting the total alkaloids of Duckbill flower, and there is no report on the research of extracting high-purity total alkaloids from Duckbill flower. The present invention effectively reduces the effect of cellulose and starch substances on the extraction through microbial fermentation. interference, and then extracted with ethanol solution, filtered, degreased, concentrated under reduced pressure, affinity chromatography, ion exchange chromatography, crystallization, the purity of the total alkaloids obtained from Duckbill flower was higher, and the extraction efficiency of the total alkaloids was also improved. , which will provide a better foundation for the utilization of natural plant resources and the modernization of traditional Chinese medicine utilization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The dried duckbill stems and leaves are pulverized to 10-20 meshes, 100Kg is weighed, 10L of Aspergillus serratus culture solution is added, and aerobic solid fermentation is carried out at 28-30℃ for 3-5 days. After fermentation, 800L of 60-80% ethanol was added to the duckbill flower, and ultrasonic extraction was carried out at room temperature for 3 times, 30 minutes each time, filtered twice with three layers of gauze and once with ceramic membrane, combined the filtrate, and concentrated under reduced pressure at 50°C to obtain a dry product 4.22kg; extract the dried material with petroleum ether, filter once with ceramic membrane, and concentrate the filtrate at 50℃ under reduced pressure to recover petroleum ether; obtain 3.71kg of filter residue, add pH=9 alkaline water to dissolve, filter once with ceramic membrane, The filtrate was adsorbed and separated on the macroporous adsorption resin D101, and the collected 80% ethanol phase eluate was concentrated and dr...

Embodiment 2

[0032] The dried duckbill stems and leaves are crushed to 10-20 meshes, 200Kg is weighed, and 15L of Aspergillus sphaerospora culture solution is added, and aerobic solid fermentation is carried out at 28-30°C for 3 to 5 days. After fermentation, 800L of 60-80% ethanol was added to the duckbill flower, and ultrasonic extraction was carried out at room temperature for 3 times, 30 minutes each time, filtered twice with three layers of gauze and once with ceramic membrane, combined the filtrate, and concentrated under reduced pressure at 50°C to obtain a dry product 8.46kg; extract the dried substance with petroleum ether, filter once with ceramic membrane, and concentrate the filtrate at 50℃ under reduced pressure to recover petroleum ether; obtain 7.47kg of filter residue, add pH=9 alkaline water to dissolve, filter once with ceramic membrane, The filtrate was adsorbed and separated by macroporous adsorption resin D101, and the collected 80% ethanol phase eluate was concentrated ...

Embodiment 3

[0035] The dried duckbill stems and leaves are crushed to 10-20 meshes, 5Kg is weighed, 0.5L of Aspergillus serratus culture solution is added, and aerobic solid fermentation is carried out at 28-30°C for 3 to 5 days. After fermentation, 800L of 60-80% ethanol was added to the duckbill flower, and ultrasonic extraction was carried out at room temperature for 3 times, 30 minutes each time, filtered twice with three layers of gauze and once with ceramic membrane, combined the filtrate, and concentrated under reduced pressure at 50°C to obtain a dry product 211kg; extract the dried substance with petroleum ether, filter once with ceramic membrane, and concentrate the filtrate at 50℃ under reduced pressure to recover petroleum ether; get 184g of filter residue, add pH=9 alkaline water to dissolve, filter once with ceramic membrane, and filter on the filtrate The macroporous adsorption resin D101 is used for adsorption separation, and the collected 80% ethanol phase eluate is concent...

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Abstract

The invention aims to provide a method for extracting high-purity total alkaloids from justicia adhatoda. The method has the characteristics of simple operation, low cost, convenience in industrial production, high purity of acquired total alkaloids, and the like. The method comprises the following steps: performing microbial fermentation and then extracting by using ethanol solution, filtering, degreasing, performing vacuum concentration, performing affinity chromatography and ion-exchange column chromatography and crystallizing, thereby acquiring a pure product of the total alkaloids of the justicia adhatoda. The method is simple and convenient in operation and is suitable for industrial operation. The purity of the acquired product is 90% or above.

Description

Technical field [0001] The invention belongs to the field of natural product processing and relates to a method for extracting high-purity total alkaloids from duckbill flowers. Background technique [0002] Duckbills are also called big resurrection, big pierced bones, big elders, etc. It is an evergreen shrub of Acanthaceae Duckbills, mainly distributed in Southeast Asia, in Guangdong, Guangxi, Hainan, Macau, Hong Kong, Yunnan and other places in my country. It is productive, cultivated, or wild. The main characteristics of the plant are: large shrubs, up to 1-3 meters; the branches are cylindrical, gray, with lenticels, and the tender branches are densely covered with gray and white puberulent. The leaves are papery, rectangular-lanceolate to lanceolate, or ovate or elliptic-ovate, 15-20 cm long, 4.5-7.5 cm wide, acuminate at the tip, sometimes slightly caudate, broadly cuneate at the base, Entire, nearly glabrous on the top, puberulent on the back; midvein in the upper groov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/19
CPCA61K36/19A61K2236/19A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 陈宏伟
Owner SUZHOU UNIV OF SCI & TECH
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