A kind of biological mortar preparation method and its device
A biological and mortar technology, applied in bacteria and other directions, can solve the problems of single nutrient solution composition and unfavorable environmental protection, and achieve the effect of enhancing effect, improving biological curing efficiency and improving preparation effect.
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Embodiment 1
[0026] Such as figure 1 Described, a kind of biological mortar preparation device prepares the method for mortar, comprises the steps:
[0027] S1: Preparation of culture medium: select 20g / L yeast extract, 10g / L ammonium sulfate, and 2g / L sodium hydroxide to mix to obtain culture medium, and adjust the pH value of the culture medium to 9. Ultraviolet sterilization after high temperature sterilization;
[0028] S2: Preparation of bacterial solution: After activating the biological strains through the plate, add the strains to the culture solution obtained in step S1, place the strains in a water bath constant temperature oscillation box after the inoculation, set the temperature at 27°C, and rotate at 160rpm. After 16 hours of continuous culture, if white turbidity appears, the culture of inoculated bacteria is successful;
[0029] S3: Biological mortar nutrient solution preparation: in deionized water, add urea 0.5mol / L, calcium chloride 0.5mol / L, ammonium chloride 10g / L, n...
Embodiment 2
[0037] Such as figure 1 Described, a kind of biological mortar preparation device prepares the method for mortar, comprises the steps:
[0038] S1: Preparation of culture medium: select 20.3g / L yeast extract, 10.2g / L ammonium sulfate, and 2.03g / L sodium hydroxide to mix to obtain culture medium, adjust the pH value of the culture medium to 9.1, and match The nutrient solution is sterilized by high temperature and then sterilized by ultraviolet light;
[0039] S2: Preparation of bacterial solution: After activating the biological strains through the plate, add the strains to the culture solution obtained in step S1, place the strains in a water bath constant temperature oscillation box after the inoculation, set the temperature at 30°C, and rotate at a speed of 160- 180rpm, after 16-19 hours of continuous culture, if white turbidity appears, the culture of inoculated bacteria is successful;
[0040] S3: Biological mortar nutrient solution preparation: in deionized water, add ...
Embodiment 3
[0047] S1: Preparation of culture medium and S2: Preparation of bacterial liquid:
[0048] The microorganisms are Sporosarcina pasteurii purchased from DSM Company in the Netherlands, numbered DSM33. The bacterium is spherical or oval, has a few flagellar movements, is Gram-positive, and is a chemoheterotrophic bacterium.
[0049] The biological strains are purchased as dry freeze powder, and after being activated on a plate, they are then expanded and cultivated. The composition of the culture solution for the expanded culture: 20g / L yeast extract, 10g / L ammonium sulfate, 2g / L sodium hydroxide (adjust pH=9). The prepared nutrient solution was put into a high-temperature sterilization pot for sterilization, the temperature was controlled at 121°C, and the sterilization time was 20 minutes. After sterilization, put the Erlenmeyer flask in the ultra-clean workbench and then carry out ultraviolet sterilization. After sterilization, use a sterilized inoculation rod to inoculate o...
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