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Combined microbial inoculum for straw fermentation and preparation method thereof

A technology for combining microbial inoculants and microorganisms, which is applied in the field of microbial inoculants for straw fermentation and its preparation, can solve the problems of single type of microbial inoculants, low total cellulose degradation rate, long fermentation period and the like, and achieves simplified preparation steps. , The formula is reasonable, and the effect of recycling is realized

Inactive Publication Date: 2017-11-24
WUHU WANJIANG INTPROP OPERATION CENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microbial agents used in the straw fermentation and degradation process at this stage have problems such as single species, weak decomposition ability, low total degradation rate of cellulose, and long fermentation cycle.

Method used

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  • Combined microbial inoculum for straw fermentation and preparation method thereof
  • Combined microbial inoculum for straw fermentation and preparation method thereof
  • Combined microbial inoculum for straw fermentation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A microbial combination bacterial agent for straw fermentation includes raw materials and parts by weight as in Example 1 of Table 1, and its preparation method is:

[0033] (1) Inoculate Corning wood enzyme, Lactobacillus acidophilus, Candida utilis, Phanerochaete chrysosporium, Pediococcus lactis, glutamic acid fermentation bacteria, Trichoderma harzianum, Paecilomyces lilacinus respectively in Cultivate and ripen for 15 hours at 25°C in the liquid medium; the liquid medium contains the following components and weight percentages per liter of medium: 5.0% glucose, 3.0% amino acid, 2.0% beef extract, 1.5% potassium dihydrogen phosphate, Sodium chloride 0.5%, the rest is distilled water, pH is 6.8.

[0034] (2) Place the strains cultivated in step (1) in fermenters respectively, and mass-produce the strains of Trichoderma koningenzyme, Lactobacillus acidophilus, glutamic acid fermenting bacteria and Paecilomyces lilacinus cultured in the fermentation broth The number i...

Embodiment 2

[0040] A microbial composition bacterial agent for straw fermentation includes raw materials and parts by weight as in Example 2 of Table 1, and its preparation method is:

[0041] (1) Inoculate Corning wood enzyme, Lactobacillus acidophilus, Candida utilis, Phanerochaete chrysosporium, Pediococcus lactis, glutamic acid fermentation bacteria, Trichoderma harzianum, Paecilomyces lilacinus respectively in Cultivate and ripen for 12 hours at 28°C in a liquid medium; the liquid medium contains the following components and weight percentages per liter of medium: 5.0% glucose, 3.0% amino acid, 2.0% beef extract, 1.5% potassium dihydrogen phosphate, Sodium chloride 0.5%, the rest is distilled water, pH is 6.8.

[0042](2) Place the strains cultivated in step (1) in fermenters respectively, and mass-produce the strains of Trichoderma koningenzyme, Lactobacillus acidophilus, glutamic acid fermenting bacteria and Paecilomyces lilacinus cultured in the fermentation broth The number is 5...

Embodiment 3

[0046] A microbial composition bacterial agent for straw fermentation includes raw materials and parts by weight as in Example 3 of Table 1, and its preparation method is:

[0047] (1) Inoculate Corning wood enzyme, Lactobacillus acidophilus, Candida utilis, Phanerochaete chrysosporium, Pediococcus lactis, glutamic acid fermentation bacteria, Trichoderma harzianum, Paecilomyces lilacinus respectively in Cultivate and ripen for 10 hours at 30°C in a liquid medium; the liquid medium contains the following components and weight percentages per liter of medium: 5.0% glucose, 3.0% amino acid, 2.0% beef extract, 1.5% potassium dihydrogen phosphate, Sodium chloride 0.5%, the rest is distilled water, pH is 6.8.

[0048] (2) Place the strains cultivated in step (1) in fermenters respectively, and mass-produce the strains of Trichoderma koningenzyme, Lactobacillus acidophilus, glutamic acid fermenting bacteria and Paecilomyces lilacinus cultured in the fermentation broth The number is ...

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Abstract

The invention provides a combined microbial inoculum for straw fermentation and a preparation method thereof. The combined microbial inoculum comprises the following culture raw materials in parts by weight: 15-30 parts of trichoderma koningii, 15-30 parts of lactobacillus acidophilus, 10-20 parts of candida utilis, 5-10 parts of phanerochaete chrysosporium, 15-30 parts of pediococcus acidilactici, 10-20 parts of glutamic acid fermenting bacteria, 10-20 parts of trichoderma harzianum and 5-15 parts of paecilomyces lilacinus. The combined microbial inoculum capable of quickly fermenting straws is obtained by separately culturing and curing a variety of functional strains capable of efficiently producing ligninase, cellulose and hemicellulose, and then performing cross antagonistic culture; a good synergistic effect is established among the cultures of the combined microbial inoculum, so that the combined microbial inoculum can efficiently ferment and decompose a variety of crop straws.

Description

technical field [0001] The invention relates to a microbial combination bacterial agent for straw fermentation and a preparation method thereof. Background technique [0002] As a large agricultural country, my country is rich in crop straw resources, with a huge quantity and various types. According to statistics, the annual output of crop straw is more than 700 million tons, including 230 million tons of rice straw, 220 million tons of corn straw, beans and potatoes, etc. 300 million tons, accounting for about 30% of the world's total straw. [0003] Because straw is not easy to decompose in a short period of time, straw resources have not been rationally developed for a long time. Except for a small amount used for gaskets, feeding livestock, and some used for composting, most of them are burned as living fuel, mainly in the planting industry. In some areas, crop stalks have become an obstacle in production. Therefore, straw burning spreads all over the vast countryside,...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/16C12N1/20C05G1/00C12R1/885C12R1/225C12R1/84C12R1/645C12R1/01C12R1/80C12R1/23
CPCC12N1/14C05D1/00C12N1/16C12N1/20C05F5/002C05F11/00C05F11/08
Inventor 孟迪
Owner WUHU WANJIANG INTPROP OPERATION CENT CO LTD
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