Ellagic acid prepared from solid-state fermented pomegranate peel and preparation method thereof

Abstract

The invention discloses ellagic acid prepared from solid-state fermented pomegranate peel and a preparation method thereof, and belongs to the technical field of cosmetics, medicines and healthcare products. A shallow plastic slot is used for manufacturing a fermentation bed, loading and unloading are convenient, and multi-layer stacking can be realized; aspergillus niger and Escherichia coli are mixed and fermented to form complementary effect, so that ellagic acid conversion efficiency is higher; the ellagic acid is extracted with progressively decreased dose of absolute methanol through three times, methanol is recycled, filter slag is washed with deionized water, and freeze-drying is performed to obtain a product with good quality; and the ellagic acid product is high in yield and purity. The process steps are simple, fermentation conditions are gentle, consumption of chemical substances is greatly reduced, environmental pollution is small, and large-scale production is adapted better.

Description

technical field [0001] The invention relates to ellagic acid prepared by solid-state fermentation of pomegranate peel and a method thereof, belonging to the technical fields of cosmetics, medicines and health care products. Background technique [0002] Ellagic acid, formula C 14 h 6 o 8 , which is a dimeric derivative of gallic acid, is a polyphenolic dilactone. It is a natural polyphenolic compound widely present in various nuts and fruits, especially rich in pomegranate peel. However, there are less free forms and more in condensed forms (such as ellagitannins, glycosides, etc.). Pure ellagic acid is a yellow needle-like crystal, slightly soluble in water, alcohol, soluble in hot methanol, alkali and pyridine. [0003] Research in the past 20 years has found that ellagic acid has a strong ability to resist lipid peroxidation and capture free radicals; it can inhibit the metabolic activity of carcinogens and relieve the toxicity of carcinogens; it has an inhibitory ef...

AI Technical Summary

Problems solved by technology

However, the preparation process of gallic acid (ester) is cumbersome and complicated, and the enzyme also needs to be refined and purified, and there are unknown quinones in the synthetic product, and the ratio of it to ellagic acid is 1: 2.4, which brings certain difficulties to the separation and purification of the product. Difficulties, at present, this method is rarely used for the production of ellagic acid

[0007] The third is the process of preparing ellagic acid by acid hydrolysis (patent number: 200510002081.X). This method has higher yield and purity, but it needs to consume high-concentration strong acid and complete it under long-term heating conditions. The equipment has high requirements on anti-corrosion ability and serious environmental pollution

This method uses a fermenter for solid-state fermentation, which is cumbersome to load and discharge, and the upper and lower parts of the fermentation are uneven; moreover, this method only uses a single strain of fermentation, and the efficiency needs to be improved, which has certain restrictions on large-scale industrial production.