A kind of preparation method of heterologous acellular dermal matrix

An acellular dermis and matrix technology, which is applied in the field of preparation of xenogeneic acellular dermal matrix, can solve the problems such as inability to effectively remove xenogeneic skin cells, loss of external environment protection of collagen scaffolds, and reduction of antigenicity of xenogeneic skin, so as to promote wound healing, Improve compliance and improve app performance

Active Publication Date: 2020-09-01
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing acellular dermal matrix production process has the following deficiencies: it cannot effectively remove xenogeneic skin cells, reduce the antigenicity of xenogeneic skin while retaining the complete three-dimensional structure of dermal collagen fibers; during the preparation of acellular dermal matrix, the hyaluronic Extracellular amorphous matrix components such as acid, dermatan sulfate, and heparan sulfate are also washed out along with the cell components, causing the collagen scaffold to lose its original external environmental protection. After transplanting into the wound, the degradation rate is relatively fast, and there is a tendency to partially dissolve; In addition, acellular dermal matrix is ​​prone to wound infection when used

Method used

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  • A kind of preparation method of heterologous acellular dermal matrix
  • A kind of preparation method of heterologous acellular dermal matrix
  • A kind of preparation method of heterologous acellular dermal matrix

Examples

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Embodiment 1

[0029] A method for preparing a heterogeneous acellular dermal matrix, comprising the following steps:

[0030] (1) Choose Parmesan pigskin as the skin source, depilate and disinfect the skin-taking area, remove a large piece of skin with a skin-taking machine, wash it with sterile normal saline, and take the skin drum back into a 0.30mm tomographic skin piece, Use laser drilling technology to make holes in the tomographic skin for standby;

[0031](2) soak the tomographic skin piece that step (1) makes in the dipropylene glycol dimethyl ether that contains 0.02% (v / v), concentration is in the aqueous solution of sodium o-phenylphenolate of 0.8% (m / v), process 6min, wash with sterile normal saline for 3 times, then add 2% (m / v) sodium tripolyphosphate, 0.02% (v / v) dipropylene glycol dimethyl ether and 0.2% (m / v) polyethylene glycol 15-Hydroxystearate, the concentration is 2% (m / v) trimethylolmethylaminoethanesulfonic acid aqueous solution, ultrasonic shock treatment for 8h, t...

Embodiment 2

[0037] A method for preparing a heterogeneous acellular dermal matrix, comprising the following steps:

[0038] (1) Choose Parmesan pigskin as the skin source, depilate and disinfect the skin-taking area, remove a large piece of skin with a skin-taking machine, wash it with sterile normal saline, and take the skin drum back into a 0.35mm tomographic skin piece, Use laser drilling technology to make holes in the tomographic skin for standby;

[0039] (2) Soak the tomographic skin piece that step (1) makes in the dipropylene glycol dimethyl ether that contains 0.04% (v / v), concentration is in the aqueous solution of sodium o-phenylphenolate of 0.6% (m / v), process 8min, wash with sterile normal saline 4 times, then add 3% (m / v) sodium tripolyphosphate, 0.02% (v / v) dipropylene glycol dimethyl ether and 0.1% (m / v) polyethylene glycol 15-Hydroxystearate, the concentration is 2% (m / v) trimethylolmethylaminoethanesulfonic acid aqueous solution, ultrasonic shock treatment for 12h, the...

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Abstract

The invention relates to a preparation method of heterogeneous acellular dermal matrix. The preparation method comprises the following steps: taking bama miniature pig skin as a skin source, reversely taking split-thickness skin by adopting a skin drum, carrying out the steps of removing epidermis and extracting cells, thus obtaining acellular dermis, enabling the acellular dermis to react with carboxymethyl chitosan, thus obtaining acellular dermal matrix, finally, treating the acellular dermal matrix by adopting a softening agent and a humectant, so that the prepared acellular dermal matrix is closer to the natural state of the skin of the human body, and thus the use compliance of the acellular dermal matrix is improved. The heterogeneous acellular dermal matrix prepared by adopting the method disclosed by the invention is low in immunogenicity, meanwhile, the heterogeneous acellular dermal matrix keeps the three-dimensional stereoscopic structure of dermal collagen fibers, improves the stability of the three-dimensional structure of the dermal collagen fibers, and has the excellent biocompatibility.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering skin, and in particular relates to a preparation method of heterogeneous acellular dermal matrix. Background technique [0002] Skin defects caused by burns, trauma, epithelial cancer resection, and skin diseases often leave scar hyperplasia and contracture deformities, which seriously affect the appearance and function. At present, in addition to autologous skin and skin flaps, the repair materials used to repair wounds mainly include allogeneic skin, heterogeneous skin, and various synthetic wound coverings. In the treatment of patients with extensive burns, the patient’s own skin source is limited, so these skin substitutes need to be used; the source of allogeneic skin is limited, there is a risk of infectious diseases, and it is restricted by ethics; Poor infectivity and expensive. Therefore, it is currently a research hotspot at home and abroad to seek a heterogeneous dermis tha...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/36A61L27/50A61L27/54A61L27/60
CPCA61L27/362A61L27/3687A61L27/3691A61L27/50A61L27/54A61L27/60A61L2300/404A61L2300/412A61L2430/40
Inventor 车七石
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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