Feed for penaeus vannamei at oogenesis period
A technology of Penaeus vannamei feed, which is applied in the field of Penaeus vannamei feed during the oviposition period, can solve the problems of no pollution to the environment, residues, and difficult digestion and absorption of feed, and achieve the effects of increasing the amount of eggs, reducing residues, and reducing ammonia nitrogen content
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Embodiment 1
[0014] Penaeus vannamei feed during the ovipositing stage, the feed ingredients and parts by weight are: 12 parts of fish meal, 20 parts of fermented peanut meal, 3 parts of fish oil, 2 parts of soybean lecithin oil, 40 parts of flour, 0.1 part of choline chloride, 0.8 parts of brewer's yeast part, bile salt 0.1 part. The preparation of the feed includes the following steps:
[0015] 1) Activation culture: first inoculate Bacillus subtilis on the slant medium in an aseptic manner, and culture at 29°C for 2 days. The components and parts by weight of the slant medium are: 0.2 parts of beef extract, 1 part of peptone, sodium chloride 0.4 parts, 2 parts of agar, 90 parts of water; then pick 1 ring of bacteria from the slant culture medium and put it into the liquid activation medium, shake it at 32°C and 170r / min for 6h, the components and weight parts of the liquid activation medium are : 1.4 parts of beef extract, 5 parts of peptone, 2.4 parts of sodium chloride, 400 parts of ...
Embodiment 2
[0019] Penaeus vannamei feed during the ovipositing stage, the feed ingredients and parts by weight are: 16 parts of fish meal, 24 parts of fermented peanut meal, 5 parts of fish oil, 5 parts of soybean lecithin oil, 45 parts of flour, 0.3 parts of choline chloride, 1.2 parts of brewer's yeast part, bile salt 0.2 part. The preparation of the feed includes the following steps: first, inoculate Bacillus subtilis on the slant medium in an aseptic manner, and cultivate it at 33°C for 3 days. 0.7 parts of sodium chloride, 3 parts of agar, 95 parts of water; then pick 2 rings of bacteria from the slant culture medium and insert them into the liquid activation medium, shake and culture at 37°C and 180r / min for 7h, the composition and weight of the liquid activation medium The parts are: 2.5 parts of beef extract, 7 parts of peptone, 3.5 parts of sodium chloride, and 450 parts of water. The Bacillus subtilis used was purchased from China Agricultural Microorganism Culture Collection ...
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