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Method and equipment suitable for laboratory culture of bacteriorhodopsin on large scale

A technology of Rhodobacter sphaeroides and cultivating equipment, applied in the field of microorganisms, can solve problems such as being unsuitable for use in ordinary laboratories, and achieve the effects of intelligent operation and control, easy management, and scientific and reasonable design.

Pending Publication Date: 2017-12-08
LINYI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There are many ways to cultivate Rhodobacter sphaeroides, but they are all industrialized production methods using fermentation tanks ["Food Machinery" September 2013 No. 29 No. 5 P56-59], which is not suitable for ordinary laboratories.

Method used

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  • Method and equipment suitable for laboratory culture of bacteriorhodopsin on large scale
  • Method and equipment suitable for laboratory culture of bacteriorhodopsin on large scale

Examples

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Effect test

Embodiment 1

[0040] A method suitable for cultivating Rhodobacter sphaeroides on a laboratory scale, mainly comprising the following steps:

[0041] S1. Preparation of culture medium:

[0042] a. Preparation of trace element solution (0.5L): EDTA8.825g 11.25g, ZnSO 4 ·7H 2 O 54.75g, FeSO 4 ·7H 2 O 25g, MnSO4.H2O 7.7g, CuSO 4 ·5H 2 O 1.96g, Co(NO 3 )·6H 2 O 1.24g, H 3 BO 4 0.57g; add H dropwise if necessary 2 SO 4 Store at 4°C until the solution becomes clear;

[0043] b. Preparation of liquid A (1L): 4g of succinic acid, KH 2 PO 4 3.4g, NaCl 0.5g, (NH4) 2 SO 4 0.5g, trisodium nitrilotriacetate 0.2g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.002g, trace element solution 0.1ml, 1% (NH 4 )6Mo 7 o 24 4H 2 O 0.02ml; adjust pH to 7 with 8M NaKOH (240gNaOH+112.22gKOH+934.6ml distilled water), store at 4°C;

[0044] c. Preparation of liquid B (1L): 36.8g of L-glutamic acid, 2g of L-aspartic acid, 100g of casamino acids, CaCl 2 2H 2 O 1.67g; adjust pH to 7.2 with 8M NaKOH, s...

Embodiment 2

[0063] A method suitable for cultivating Rhodobacter sphaeroides on a laboratory scale, mainly comprising the following steps:

[0064] S1. Preparation of culture medium:

[0065] a. Preparation of trace element solution (0.5L): Na 2 EDTA·2H 2 O 11.25g, ZnSO 4 ·7H 2 O 54.75g, FeSO 4 ·7H 2 O 25g, MnSO4.H2O 7.7g, CuSO 4 ·5H 2 O 1.96g, Co(NO 3 )·6H 2 O 1.24g, H 3 BO 4 0.57g; add H dropwise if necessary 2 SO 4 Store at 4°C until the solution becomes clear;

[0066] b. Preparation of liquid A (1L): sodium succinate 9.14g, KH 2 PO 4 3.4g, NaCl 0.5g, (NH4) 2 SO 4 0.5g, trisodium nitrilotriacetate 0.2g, MgSO 4 ·7H 2 O 0.3g, FeSO 4 ·7H 2 O 0.002g, trace element solution 0.1ml, 1% (NH 4 )6Mo 7 o 24 4H 2 O 0.02ml; adjust pH to 7 with hydrochloric acid, store at 4°C;

[0067]c. Preparation of liquid B (1L): 36.8g of L-glutamic acid, 2g of L-aspartic acid, 100g of casamino acids, CaCl 2 2H 2 O 1.67g; adjust pH to 7.2 with 8M NaKOH, store at 4°C;

[0068] d. M...

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Abstract

The invention discloses a method and equipment suitable for laboratory culture of bacteriorhodopsin on a large scale. The method primarily comprises the following steps: S1, preparing a culture liquor: a, preparing a trace element solution; b, preparing a liquid A; c, preparing a liquid B, and d, mixing the liquid A and the liquid B; S2, culture process: a, enlarge cultivation, b, performing culture in a culture pond till the OD value reaches 2.0; and c, strain storage; and S3, harvesting thalli. The equipment primarily comprises a culture pond and a constant temperature circulator. The method suitable for laboratory culture of bacteriorhodopsin on a large scale is simple, efficient and low in production cost and suitable for common laboratory utilization. The culture equipment used by the method is scientific and reasonable in design, can realize intelligent operation and control, is convenient to use and manage, and has relatively high practical value.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a method and equipment suitable for large-scale cultivation of Rhodobacter sphaeroides in a laboratory. Background technique [0002] Rhodobacter sphaeroides belongs to the purple non-sulfur bacteria and is one of the most intensively studied photosynthetic microorganisms. It can grow under a variety of conditions, such as aerobic, anaerobic, light and dark. Rhodobacter sphaeroides plays an important role in studying the mechanism of photosynthesis and respiration, and has become a model organism. In addition, Rhodobacter sphaeroides can produce coenzyme Q 10 , carotenoids, superoxide dismutase (SOD), 5-aminolevulinic acid (ALA), hydrogen and D-psicose, etc., and can also degrade pesticide residues, organic wastewater and polycyclic aromatic hydrocarbons (PAHs), etc. , can also fix carbon dioxide and nitrogen and remediate radioactive contamination. It can be seen that ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12M1/38C12M1/36C12M1/34C12M1/02C12R1/01
CPCC12M23/02C12M41/00C12M41/18C12N1/20
Inventor 曲元刚
Owner LINYI UNIVERSITY
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