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Water-soluble quercetin nanometer particles and preparation method thereof

A nanoparticle, quercetin technology, applied in the field of quercetin, can solve the problems of low solubility of quercetin, weak chemical stability, complicated preparation process, etc., and achieves good colloidal stability and chemical stability, good solubility, Good colloidal stability

Active Publication Date: 2017-12-15
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] This method overcomes the problems of extremely low solubility of quercetin in water, poor dispersibility, and weak chemical stability. The existing methods for preparing quercetin nanoparticles have defects such as complicated preparation process, large particle size, and expensive carrier raw materials. Molecules and small molecules interact non-covalently, and form nano-colloidal structures by manipulating simple process conditions. The prepared quercetin nanoparticles have good solubility, small average particle size, good monodispersity, and good colloidal and chemical stability.

Method used

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  • Water-soluble quercetin nanometer particles and preparation method thereof
  • Water-soluble quercetin nanometer particles and preparation method thereof
  • Water-soluble quercetin nanometer particles and preparation method thereof

Examples

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Embodiment 1

[0042] A preparation method of water-soluble quercetin nanoparticles, comprising the steps of:

[0043] Step 1: uniformly disperse wheat protein in deionized water to prepare a 5% (w / v, g / mL) dispersion. The dispersion solution was heated in a water bath to 37° C., adjusted to pH 9.0 with 1M NaOH, and trypsin was added at a mass ratio of 1% for enzymolysis. Under the conditions of maintaining a constant temperature and pH, the enzymolysis was carried out for 1 hour. After the enzymatic hydrolysis, the pH of the material was adjusted to 7.5 with 1M HCl, and then heated in a boiling water bath for 10 minutes to inactivate the enzyme. After the hydrolyzate was centrifuged at 10,000r / min for 20min, the supernatant was passed through a 0.45μm microfiltration membrane and put into a dialysis bag with a molecular weight cutoff of 14kDa, and dialyzed in deionized water for 3d at 4°C (1d: deionized water ; 2d: 0.05% glacial acetic acid; 3d: deionized water), the dialysate was replaced...

Embodiment 2

[0046] A preparation method of water-soluble quercetin nanoparticles, the enzymolysis time is controlled to be 2h, and the others are the same as in Example 1.

Embodiment 3

[0048] A preparation method of water-soluble quercetin nanoparticles, the enzymolysis time is controlled to be 3h, and the others are the same as in Example 1.

[0049] figure 1 It is a solution observation diagram of Que solubilization by PBS solution and enzymolysis 1h, 2h, 3h, that is, the quercetin nanoparticle dispersion prepared in Comparative Example 1 and Examples 1-3. Depend on figure 1 It can be seen that the solubilization of quercetin by enzymolysis products with different enzymatic hydrolysis time is different. When it is a PBS solution (that is, the enzymatic hydrolysis time is 0 h), quercetin is a turbid solution in the aqueous solution, and flocs are floating in the solution. With the prolongation of the enzymatic hydrolysis time to 1~3h, the compound solution of quercetin and the enzymatic hydrolyzate was gradually clarified. After 2h of enzymatic hydrolysis, the solution basically did not appear precipitation. Better amphiphilic polypeptide, the polypeptid...

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Abstract

The invention discloses water-soluble quercetin nanometer particles and a preparation method thereof. The preparation method comprises the following steps: firstly, enabling wheat protein to be uniformly dispersed in deionized water, performing heating, adjusting the pH value, adding trypsin, performing enzymolysis, performing enzyme deactivation, and performing dialysis so as to obtain wheat protein zymolyte; then, selecting an organic solvent for compounding quercetin mother liquid; enabling the wheat protein hydrolyzate to dissolve in a PBS solution; dropwise adding the quercetin mother liquid in the wheat protein hydrolyzate solution, and performing centrifugation so as to obtain a wheat protein hydrolyzate-quercetin nanometer particle solution; and performing freeze-drying so as to obtain the water-soluble quercetin nanometer particles. According to the water-soluble quercetin nanometer particles and the preparation method thereof disclosed by the invention, the water-soluble quercetin nanometer particles are prepared through the non-covalence interaction between the wheat protein hydrolysate and quercetin. The water-soluble quercetin nanometer particle products are good in solubility, small in particle diameter, high in load rate and stable in chemical stability and colloid stability, the processing stability of the quercetin is greatly improved, and the water-soluble quercetin nanometer particles can be applied as food ingredients.

Description

technical field [0001] The invention relates to quercetin, in particular to a water-soluble quercetin nano particle and a preparation method thereof; the water-soluble quercetin nano particle can be used in health food and functional food. Background technique [0002] Quercetin (Quercetin, Que) is a class of flavonoids derived from leguminous plants. It can play a certain therapeutic role in the treatment of chronic bronchitis, coronary heart disease, hypertension and other diseases. It is a class of very valuable nutritionally active ingredients. However, due to the poor solubility and chemical stability of Que in water, its application in the biological and food fields is limited. Studies have shown that the nanoparticle delivery system can effectively improve the water solubility and chemical stability of hydrophobic substances, and is an effective means of delivering hydrophobic active substances. At present, the commonly used method is to use the active groups of pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L33/185A23P10/30
CPCA23V2002/00A23L33/105A23L33/185A23P10/30A23V2250/21168
Inventor 王金梅韦翠兰欧阳颖
Owner SOUTH CHINA UNIV OF TECH
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