Water-soluble quercetin nanometer particles and preparation method thereof
A nanoparticle, quercetin technology, applied in the field of quercetin, can solve the problems of low solubility of quercetin, weak chemical stability, complicated preparation process, etc., and achieves good colloidal stability and chemical stability, good solubility, Good colloidal stability
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Embodiment 1
[0042] A preparation method of water-soluble quercetin nanoparticles, comprising the steps of:
[0043] Step 1: uniformly disperse wheat protein in deionized water to prepare a 5% (w / v, g / mL) dispersion. The dispersion solution was heated in a water bath to 37° C., adjusted to pH 9.0 with 1M NaOH, and trypsin was added at a mass ratio of 1% for enzymolysis. Under the conditions of maintaining a constant temperature and pH, the enzymolysis was carried out for 1 hour. After the enzymatic hydrolysis, the pH of the material was adjusted to 7.5 with 1M HCl, and then heated in a boiling water bath for 10 minutes to inactivate the enzyme. After the hydrolyzate was centrifuged at 10,000r / min for 20min, the supernatant was passed through a 0.45μm microfiltration membrane and put into a dialysis bag with a molecular weight cutoff of 14kDa, and dialyzed in deionized water for 3d at 4°C (1d: deionized water ; 2d: 0.05% glacial acetic acid; 3d: deionized water), the dialysate was replaced...
Embodiment 2
[0046] A preparation method of water-soluble quercetin nanoparticles, the enzymolysis time is controlled to be 2h, and the others are the same as in Example 1.
Embodiment 3
[0048] A preparation method of water-soluble quercetin nanoparticles, the enzymolysis time is controlled to be 3h, and the others are the same as in Example 1.
[0049] figure 1 It is a solution observation diagram of Que solubilization by PBS solution and enzymolysis 1h, 2h, 3h, that is, the quercetin nanoparticle dispersion prepared in Comparative Example 1 and Examples 1-3. Depend on figure 1 It can be seen that the solubilization of quercetin by enzymolysis products with different enzymatic hydrolysis time is different. When it is a PBS solution (that is, the enzymatic hydrolysis time is 0 h), quercetin is a turbid solution in the aqueous solution, and flocs are floating in the solution. With the prolongation of the enzymatic hydrolysis time to 1~3h, the compound solution of quercetin and the enzymatic hydrolyzate was gradually clarified. After 2h of enzymatic hydrolysis, the solution basically did not appear precipitation. Better amphiphilic polypeptide, the polypeptid...
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