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Coumarin derivative, preparation and application of coumarin derivative, and bisulfite detection kit

A bisulfite and coumarin-based technology, applied in chemical instruments and methods, measuring devices, color/spectral characteristics measurement, etc., can solve the problem that the sensitivity is not enough to meet the detection requirements of samples

Inactive Publication Date: 2017-12-15
HEBEI UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, some of these methods require complex instruments and equipment, some require complex sample pretreatment, and some are not sensitive enough to meet the detection requirements of the sample. Therefore, a new analytical method for the detection of sulfite with good selectivity, convenience and high sensitivity has been developed. of great significance

Method used

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  • Coumarin derivative, preparation and application of coumarin derivative, and bisulfite detection kit
  • Coumarin derivative, preparation and application of coumarin derivative, and bisulfite detection kit
  • Coumarin derivative, preparation and application of coumarin derivative, and bisulfite detection kit

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Embodiment 1

[0054] A kind of coumarin derivative, its chemical name is 2-((1E,3Z)-4-chloro-4-(6-diethylamino)-2-oxo-2H-chroman -3) butane-1,3-diene-1)-3-methylbenzo[d]thiazolium-ium iodide (2-((1E,3Z)-4-chloro-4-(6-( diethylamino)-2-oxo-2H-chromen-3-yl)buta-1,3-dien-1-yl)-3-methylbenzo[d]thiazol-3-ium), its structural formula is as follows:

[0055] .

[0056] The preparation method of coumarin derivatives shown in formula (I) comprises the following steps:

[0057] (1) Dissolve 5mmol 4-(diethylamino)salicylaldehyde and 5mmol ethyl acetoacetate in 20mL ethanol, add 0.1mL piperidine, stir and reflux the resulting mixture for 8h, stop the reaction, cool to room temperature, and filter to obtain the compound 1 (3-acetyl-7-(diethylamino)-2H-chroman-2-one, 3-acetyl-7-(diethylamino)-2H-chromen-2-one);

[0058] (2) Stir 2 mL of DMF and 2 mL of phosphorus oxychloride at 0°C for 30 min, and add dropwise to compound 1 (3-acetyl-7-(diethylamino)-2H-benzobis) dissolved in 20 mL of DMF Hydropyra...

Embodiment 2

[0067] Prepare HEPES buffer solution with pH = 7.4 and a concentration of 10mM for use; prepare a DMF solution of coumarin derivatives shown in formula (I) with a concentration of 2mM for use; prepare 1000µL of Add DMF solution, 1000µL HEPES buffer solution and 10µL DMF solution of coumarin derivatives shown in formula (I) to a clean UV cuvette, and detect it on a UV-Vis spectrophotometer while adding samples. With the addition of hydrogen sulfate solution, the absorption peak at 545nm gradually decreases, and the absorption peak at 424nm gradually increases, see figure 1 .

Embodiment 3

[0069] Configure pH = 7.4, HEPES buffer solution with a concentration of 10mM, for standby; configure a DMF solution of coumarin derivatives shown in formula (I) with a concentration of 2mM, for standby; mix 1000µL DMF solution, 1000µL HEPES buffer solution and 10µL formula ( I) Add the DMF solution of the coumarin derivatives shown in a clean UV cuvette, and then gradually add standard aqueous bisulfite (HSO 3- Concentration is 2mM), when the accumulatively added volumes of standard bisulfite aqueous solution are 0µL, 2µL, 4µL, 6µL, 8µL, 10µL, 12µL, 14µL, 16µL, 18µL, 20µL, the UV absorption curves were detected respectively, and the results Such as figure 1 As shown, the absorption peaks corresponding to 545nm measured on a UV-visible spectrophotometer are 0.646, 0.379, 0.225, 0.153, 0.111, 0.087, 0.07, 0.059, 0.051, 0.046, 0.041; 424nm The corresponding absorption peaks are 0.099, 0.210, 0.281, 0.315, 0.333, 0.346, 0.353, 0.359, 0.362, 0.365, 0.366;

[0070] Then take the...

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Abstract

The invention provides a coumarin derivative (2-((1E,3Z)-4-chloro-4-(6-(diethylamino)-2-oxo-2H-chromen-3-yl)buta-1,3-dien-1-yl)-3-methylbenzo[d]thiazol-3-ium), a preparation method and an application of the coumarin derivative, and a bisulfite detection kit. The coumarin derivative can be used to detect the concentration of a bisulfite ion in a sample by using an ultraviolet spectroscopy or fluorescent spectrometry. The invention prepares the novel coumarin derivative, the compound can serve as a detection reagent of the bisulfite ion and shows quite high sensitivity and selectivity for the bisulfite ion, the detection result is not affected by other metal ions and is accurate, the detection process is convenient, sensitive and fast, the limit of detection is relatively low, the linear range is relatively wide, and the detection method is simple, low in cost, and easy to realize, and has a broad range of application.

Description

technical field [0001] The invention relates to the detection and analysis of bisulfite, in particular to a coumarin derivative, its preparation method and application, and a bisulfite detection kit. Background technique [0002] Because bisulfite can prevent oxidation and bacterial growth and control enzyme reactions, it has become the most basic preservative for many foods, beverages and pharmaceutical products. It is often used in food or wine to prevent oxidation, browning, bacterial fermentation and other processes , but bisulfite ions are irritating to human skin, eyes, and respiratory tract, and a certain concentration of bisulfite can cause asthma and allergies in some individuals. Therefore, the content of bisulfite and sulfur dioxide in food and medicine needs to be strictly controlled within a safe range. my country stipulates that the content of sulfite in sugar should not exceed 0.05mg / kg. [0003] The current methods for detecting bisulfite include optimized ...

Claims

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Application Information

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IPC IPC(8): C07D417/06C09K11/06G01N21/33G01N21/64
CPCC07D417/06C09K11/06C09K2211/1037C09K2211/1088G01N21/33G01N21/643
Inventor 李玮杨瑜涛胥稳智
Owner HEBEI UNIVERSITY
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