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Compound microorganism feed additive as well as preparation method and application thereof

A technology of compound microorganisms and feed additives, which is applied in microorganism-based methods, biochemical equipment and methods, molding or processing of animal feed, etc., can solve problems such as threats to product quality and safety, breeding failure, water pollution, etc. Safety, avoid bacterial infection, improve the effect of breeding environment

Inactive Publication Date: 2017-12-22
沧州旺发生物技术研究所(普通合伙)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Due to the excessive pursuit of high density and high yield in the aquaculture industry, it will inevitably lead to the increase and accumulation of aquaculture pollutants, leading to the gradual deterioration of the aquaculture environment, mainly reflected in: water pollution; air pollution; a large number of pathogenic microorganisms breeding and spreading diseases; drug residues; excessive trace elements Emissions will pollute the environment, etc., directly causing breeding failure and threatening product quality and safety

Method used

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  • Compound microorganism feed additive as well as preparation method and application thereof
  • Compound microorganism feed additive as well as preparation method and application thereof
  • Compound microorganism feed additive as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] A compound microbial feed additive, the compound microorganism comprises Saccharomyces cerevisiae, Bacillus subtilis, Bacillus natto, Bacillus cereus, lactic acid bacteria and photosynthetic bacteria (Photosynthetic Bacteria Abbr), and the inoculum volume ratio is 3:2:1:1:1:1.

[0045] Furthermore, the total viable bacteria content of the composite microorganism is 3×108-8×109 cfu / ml.

Embodiment 2

[0047] like figure 1 Shown, a kind of preparation method of compound microbial feed additive comprises the following steps,

[0048] (1) Preparation of solid fermentation medium

[0049] Solid fermentation medium: soybean meal, corn, bran, the addition ratio is 10:2:1;

[0050]First, the soybean meal, corn and bran are pulverized separately, passed through a 2mm sieve, and then the soybean meal, corn and bran are mixed and stirred according to the ratio of 10:2:1, and after stirring, high-pressure steam sterilization is carried out for later use;

[0051] (2) Strain slant, shake flask culture

[0052] Saccharomyces cerevisiae liquid medium YPD: peptone 20g, yeast extract 10g, glucose 20g, water 1000ml, pH6.0;

[0053] Saccharomyces cerevisiae solid medium YPD: peptone 20g, yeast extract 10g, glucose 20g, agar 20g, water 1000ml, pH6.0;

[0054] Liquid medium for other strains: yeast extract 5g, peptone 10g, NaCl 10g, water 1000ml, pH7.0;

[0055] Solid medium for other str...

Embodiment 3

[0074] Application of a compound microbial feed additive in livestock and poultry breeding.

[0075] Application test of compound microbial feed additive in livestock and poultry breeding:

[0076] 1. Application test of chicken cages

[0077] (1) Experimental design

[0078] Time: November 6, 2015 to November 8, 2016 (from the start of laying eggs to the end of culling).

[0079] Location: Layer Chicken Farm in Qing County, Hebei Province

[0080] Breeding mode: laying hens in cages

[0081] Experimental grouping: 10,000 laying hens in two henhouses in the farm were selected as test subjects for the test, and they were divided into two groups, with 5,000 laying hens in each group. One group was set as the test group, and the second group was set as the control group. The feeding conditions of the two groups of laying hens are exactly the same except that 0.1% compound microbial feed additive is added to the feed of the experimental group.

[0082] (2) Test record data an...

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Abstract

The invention provides a compound microorganism feed additive. The compound microorganism feed additive contains Saccharomyces cerevisiae, Bacillus subtilis, Bacillus natto, Bacillus cereus, Lacticacid bacteria and Photosynthetic Bacteria Abbr in an inoculation volume ratio of 3 to 2 to 1 to 1 to 1 to 1. The invention further discloses a preparation method of the compound microorganism feed additive and application of the compound microorganism feed additive in the breeding of livestock and aquatic products. By utilizing the compound microorganism feed additive provided by the invention, the breeding effects of the livestock and the aquatic products can be effectively improved, the breeding environment is improved, and the economic benefit is increased.

Description

technical field [0001] The invention belongs to the technical field of microbial feed additives and their preparation, and in particular relates to a compound microbial feed additive and its preparation method and application. Background technique [0002] Due to the excessive pursuit of high density and high yield in the aquaculture industry, it will inevitably lead to the increase and accumulation of aquaculture pollutants, leading to the gradual deterioration of the aquaculture environment, mainly reflected in: water pollution; air pollution; a large number of pathogenic microorganisms breeding and spreading diseases; drug residues; excessive trace elements Emissions will pollute the environment, etc., directly causing breeding failure and threatening product quality and safety. According to the current national policies related to food safety and environmental protection, the development and production of green, efficient, healthy, and antibiotic-free microbial feed, fee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/75A23K50/80A23K10/30A23K10/37A23K10/12A23K40/10C12N1/18C12N1/20C12R1/865C12R1/125C12R1/07C12R1/085C12R1/01
CPCA23K10/12A23K10/30A23K10/37A23K40/10A23K50/75A23K50/80C12N1/18C12N1/20Y02A40/818Y02P60/87
Inventor 张连水李春岭谷俊萍王继芬李文敏张青松王连杰宋建王明玉
Owner 沧州旺发生物技术研究所(普通合伙)
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