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Compound biological agent for restoring contaminated soil

A technology of biological agents and polluted soil, applied in the field of soil remediation, can solve the problems of small microorganisms, difficult separation of microbial cells, competition of indigenous strains, etc., and achieve the effect of improving permeability, good repairing effect, and increasing transmission volume

Active Publication Date: 2017-12-22
CHENGDU DEFEI ENVIRONMENTAL ENG CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] From the current point of view, microbial remediation is the technology with the most development potential and application prospects, but the microorganisms are small, the microbial cells enriched with heavy metals are difficult to separate from the soil, and there are also unfavorable factors such as competition with indigenous strains on the remediation site

Method used

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  • Compound biological agent for restoring contaminated soil
  • Compound biological agent for restoring contaminated soil
  • Compound biological agent for restoring contaminated soil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Enrichment of microbial flora degrading benzanthracene

[0026] The soil samples come from areas polluted by polycyclic aromatic hydrocarbons, and the sampling method is carried out by the five-point sampling method, with 200g of samples taken at each point, and a total of 1000g of samples are taken, which are mixed for testing.

[0027] Configure benzanthracene inorganic salt medium: prepare 10g / L benzanthracene solution with acetone, sterilize with a 0.25mm filter membrane, measure a certain amount of benzanthracene solution and place it in a sterilized Erlenmeyer flask until the acetone is volatilized Then add an appropriate amount of sterilized inorganic salt basal medium, so that the final concentration of benzanthracene in the inorganic salt medium is 1500 mg / L.

[0028] Take 6 conical flasks, place 150ml of liquid inorganic salt medium containing benzanthracene in each conical flask, take 5 conical flasks (A1-A5) above, add 1g of soil sample to each co...

Embodiment 2

[0031] Example 2: Isolation, identification and acquisition of microbial flora degrading benzanthracene

[0032] As can be seen from the results in Table 1, A1 and A4 culture fluids contain dominant bacterial groups that degrade benzanthracene, draw 0.1ml of the above-mentioned A1 and A4 culture fluids, dilute them in a certain ratio, and apply them to the inorganic salt solids containing benzanthracene On the medium, single colonies with different characteristics were picked, purified and inoculated on the inorganic solid salt medium containing benzanthracene, cultivated and selected the strains with faster growth, 16S rDNA was extracted by alkaline lysis method and 16S rDNA Sequence analysis (sequence analysis was completed by Shanghai Meiji Biomedical Technology Co., Ltd.), and BLAST comparison was performed on the sequencing results, and 13 strains that highly degrade benzanthracene were obtained. The names of the genus are shown in Table 2.

[0033] Table 2 The genus name...

Embodiment 3

[0048] Soil improver can break soil compaction, loosen soil, improve soil air permeability, reduce soil bulk density, promote soil microbial activity, increase soil microbial quantity, and improve enzyme activity. Microorganisms in the soil play a key role in plants, and microorganisms rely on organic carbon to grow, so the application of organic carbon soil amendments can increase the number and activity of soil microorganisms, improve enzyme activity; at the same time inhibit pathogenic fungi, bacteria, actin Bacterial activity greatly reduces the spread of soil diseases.

[0049] The composition of the compound biological preparation provided by the invention comprises by weight:

[0050] Component 1: 0.1 part of Nocardia, 0.2 part of Streptococcus sp., 0.05 part of Cytophaga sp., 0.1 part of Pseudomonadaceae sp., 0.1 part of Arthrobacter sp., Licheniform sp. 0.1 part of Bacillus licheniformis sp., 0.1 part of Mesorhzobium sp., 0.1 part of Novosphingobium sp., 0.1 part of ...

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Abstract

The invention provides a compound biological agent for restoring contaminated soil, belongs to the technical field of soil restoration and particularly relates to the compound biological agent for restoring polycyclic aromatic hydrocarbon contaminated soil. The compound biological agent provided by the invention comprises a soil conditioner which can improve the permeability of the soil and increase the transmission quantity of oxygen in the soil, thereby rapidly establishing a healthy microbial population structure. The compound biological agent further comprises a mixture of different compound microorganism strains. The compound biological agent provided by the invention has the double effects of restoring a soil ecosystem and degrading polycyclic aromatic hydrocarbons.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, and in particular relates to a compound biological preparation for remediating polycyclic aromatic hydrocarbons polluted soil. Background technique [0002] The overall situation of soil pollution in my country is grim. Heavy soil pollution areas and high-risk areas have appeared in heavily polluting enterprises or industrial-intensive areas, industrial and mining areas and surrounding areas, urban and suburban areas. There are various types of soil pollution, showing the coexistence of new and old pollutants, and the combination of inorganic and organic pollution. Organic pollutants mainly include DDT, polycyclic aromatic hydrocarbons, and wastes from nitrogen fertilizer plants, electronics plants, coking plants, chemical plants, oil production areas, and pesticide plants. Inorganic pollutants mainly include metal elements; in addition, excessive application of chemical fertilizers, pe...

Claims

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Application Information

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IPC IPC(8): B09C1/10C12N1/20C12R1/46C12R1/38C12R1/06C12R1/10C12R1/41C12R1/01C12R1/11
CPCB09C1/10C12N1/20
Inventor 董秋月
Owner CHENGDU DEFEI ENVIRONMENTAL ENG CO LTD
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