Rhizome nelumbinis polyphenol extractive and preparation method and application thereof
The technology of extract and lotus root joint is applied in the field of lotus root joint polyphenol extract and its preparation, which can solve the problems of high cost, destruction of the original flavor of food and the like, and achieve the effect of high preparation yield
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[0032] Example 1: Component Analysis and Structural Characterization of Polyphenol Extract from Lotus Rhizome
[0033] 1 Experimental materials:
[0034] 1.1 Experimental raw material: Elian No. 5 lotus root
[0035] 1.2 Experimental reagents: cellulase (10000U / g), pectinase (30000U / g), Folin-phenol reagent, Na 2 CO 3 、NaNO 2 , Al(NO 3 ) 3 and NaOH etc.
[0036] 1.3 Main instruments:
[0037] Spectrophotometer, Agilent 1100 high performance liquid chromatography mass spectrometer.
[0038] 2 Experimental method:
[0039] 2.1 Preparation of lotus root polyphenol extract:
[0040] The lotus root node was used as raw material, and the lotus root node was extracted by hydroenzymatic method with the compound enzyme of cellulase and pectinase. The mass ratio to pectinase is 2:1, the enzymatic hydrolysis time is 90min, the enzymatic hydrolysis temperature is 62.5°C, and pH=4; a 100kDa ultrafiltration membrane is used for membrane separation and purification of the water enz...
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