Rhizome nelumbinis polyphenol extractive and preparation method and application thereof

The technology of extract and lotus root joint is applied in the field of lotus root joint polyphenol extract and its preparation, which can solve the problems of high cost, destruction of the original flavor of food and the like, and achieve the effect of high preparation yield

Active Publication Date: 2017-12-22
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the problems in the prior art that the preservative preservation damages the original flavor of food, the cost is high, and it is easy to introduce external sources.

Method used

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  • Rhizome nelumbinis polyphenol extractive and preparation method and application thereof
  • Rhizome nelumbinis polyphenol extractive and preparation method and application thereof
  • Rhizome nelumbinis polyphenol extractive and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Component Analysis and Structural Characterization of Polyphenol Extract from Lotus Rhizome

[0033] 1 Experimental materials:

[0034] 1.1 Experimental raw material: Elian No. 5 lotus root

[0035] 1.2 Experimental reagents: cellulase (10000U / g), pectinase (30000U / g), Folin-phenol reagent, Na 2 CO 3 、NaNO 2 , Al(NO 3 ) 3 and NaOH etc.

[0036] 1.3 Main instruments:

[0037] Spectrophotometer, Agilent 1100 high performance liquid chromatography mass spectrometer.

[0038] 2 Experimental method:

[0039] 2.1 Preparation of lotus root polyphenol extract:

[0040] The lotus root node was used as raw material, and the lotus root node was extracted by hydroenzymatic method with the compound enzyme of cellulase and pectinase. The mass ratio to pectinase is 2:1, the enzymatic hydrolysis time is 90min, the enzymatic hydrolysis temperature is 62.5°C, and pH=4; a 100kDa ultrafiltration membrane is used for membrane separation and purification of the water enz...

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Abstract

The invention discloses a rhizome nelumbinis polyphenol extractive and a preparation method and application thereof. The rhizome nelumbinis polyphenol extractive comprises epicatechin, catechinic acid, rutin, chlorogenic acid, caffeic acid, propylgallate and procyanidine oligomer. The preparation method comprises the following steps: carrying out aqueous enzymatic method extraction on rhizome nelumbinis by compound enzyme of cellulase and pectinase to obtain a primary extractive; carrying out membrane separation on the primary extractive to obtain the rhizome nelumbinis polyphenol extractive. Rhizome nelumbinis polyphenol adopted by the rhizome nelumbinis polyphenol extractive belongs to a diet source and is taken as the substrate of polyphenol oxidase, enzymatic reaction can be regulated and controlled, an effect on inhibiting the browning of fruits and vegetables is obvious, and the rhizome nelumbinis polyphenol extractive is a good natural fruit and vegetable preservative and antioxidant. A process for preparing the rhizome nelumbinis polyphenol is characterized in that biological enzyme extraction and membrane separation purification technologies are combined, a preparation yield is relatively high, the obtained extractive is natural and is free from toxic or side effect, the whole preparation process is environmentally-friendly and low in carbon, and the rhizome nelumbinis polyphenol extractive can be widely applied to food processing.

Description

technical field [0001] The invention belongs to the field of storage and processing of agricultural products, and more specifically relates to a lotus root node polyphenol extract and its preparation method and application. Background technique [0002] Lotus root, commonly known as lotus root, belongs to the lotus family. Lotus root has a relatively large planting range in my country, and is generally distributed in Jiangsu, Anhui, Hubei, Henan, Hebei, and Zhejiang provinces. It is an important agricultural economic crop in a planting area. Lotus root tastes sweet, crisp and delicious. It can be eaten raw or cooked. It is one of the common dishes on the family table. At the same time, the medicinal value of lotus root is relatively high. Its rhizomes, leaves, flowers and fruits are all treasures and can be used for medicinal purposes. The powder made from lotus root can digest food, relieve diarrhea, appetize, clear away heat, and prevent internal bleeding. It is an excel...

Claims

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Application Information

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IPC IPC(8): C07D311/62C07H1/08C07H17/07C07C67/48C07C69/732C07C69/84C07C51/42C07C59/48A23B7/154A23L33/105A23L2/38
CPCA23B7/154A23L2/38A23L33/105C07C51/42C07C67/48C07D311/62C07H1/08C07H17/07A23V2002/00A23V2200/02A23V2200/30A23V2250/2132C07C69/732C07C69/84C07C59/48
Inventor 李书艺祝振洲王宏勋易阳闵婷
Owner WUHAN POLYTECHNIC UNIVERSITY
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