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Method for guiding cell behaviors, based on gold nanowire array

A gold nanowire and cell technology, which is applied in animal cells, nanotechnology, vertebrate cells, etc., can solve the problems of no cell regulation signal and independent research, and achieve the effect of strong controllability and easy operation

Active Publication Date: 2018-01-05
YANCHENG TEACHERS UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most properties of these materials have been studied in isolation; indeed, no cellular regulatory signal alone directs cellular assembly
Currently, there is a lack of a universal and simple operating platform to prepare precise and controllable nanopatterned surfaces for studying cell behavior

Method used

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  • Method for guiding cell behaviors, based on gold nanowire array
  • Method for guiding cell behaviors, based on gold nanowire array
  • Method for guiding cell behaviors, based on gold nanowire array

Examples

Experimental program
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Effect test

preparation example Construction

[0030] a. Preparation of substrate:

[0031] Take 8mL epoxy resin prepolymer (the volume ratio of prepolymer and curing agent is 15:2) and stir evenly, put it in a 60°C oven and heat it for 2 minutes to increase the fluidity of the prepolymer and remove the air bubbles. The body was poured into a strip-type polydimethylsiloxane (PDMS) mold prepared by soft etching technology (strip spacing was 10 μm, strip width was 10 μm, and strip height was 5 μm), and stood for 5 minutes. Then it was placed in an oven at 60° C. for 3 hours to cure, and an epoxy resin substrate with strip structure was obtained after demoulding.

[0032] b. Gold film deposition:

[0033] Place the epoxy substrate under vacuum at 5 x 10 -6 Electron beam deposition of metal gold is carried out under the vacuum degree of Pa. The deposition rate is 2nm, and then gradually increase the deposition rate to The thickness of the final deposited gold film is 100nm.

[0034] c. Preparation of sample modules:

...

Embodiment 1

[0043] Example 1 Guidance of cell behavior by three-dimensional gold nanowire array with 0° crossing angle

[0044] The substrate containing the gold nanowire structure with a crossing angle of 0° prepared in Example 5 was sterilized with 70% ethanol, and placed in a 24-well cell culture plate to stand overnight. Then, with 2×10 per well 4 Human bone marrow mesenchymal cells were added to the substrate for cell adhesion at a density of 10000000 cells. The sample is placed at a temperature of 37°C, CO 2 After 1.5 hours in an incubator with a concentration of 5%, the non-adherent cells were removed, and 1 mL of fresh medium was added to each well to continue culturing for 2 days. Cells were fixed with 3.7% paraformaldehyde in phosphate buffered saline (PBS) at room temperature for 20 minutes, washed three times with PBS, and then permeabilized with 0.5% TritonX-100 solution for 3 minutes, containing 5% bovine Serum albumin (BSA) in PBS treated cells for 30 minutes to prevent ...

Embodiment 2

[0045] Example 2 Guidance of Cell Behavior by Three-Dimensional Gold Nanowire Arrays with a Crossing Angle of 60°

[0046] Using the same method as in Example 1, the behavior of the cells was guided by the gold nanowire array with a crossing angle of 60°, and the vector response behavior of the cells was observed.

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Abstract

The invention discloses a method for guiding cell behaviors, based on a gold nanowire array. The method comprises the following steps: a, preparing a substrate; b, depositing a gold film; c, preparinga sample module; d, preparing a two-dimensional gold nanowire array structure; e, preparing a three-dimensional gold nanowire array structure; f, culturing human mesenchymal stem cells; g, guiding the cells through a gold nanowire array. The gold nanowire prepared through the method is simple in topological surface operation and high in controllability, and can accurately control the cross angleof nanowire arrays, thereby providing a universal and stable method for realizing the effective guidance of behaviors, such as cell adhesion, orientation and extension.

Description

technical field [0001] The invention relates to a method for guiding cell behavior based on a gold nanowire array. Background technique [0002] The growth of any cellular tissue benefits from the complex cellular assembly that leads to its functionality. Cell adhesion and assembly are the most fundamental and critical responses to the extracellular matrix (including composition, shape, elasticity, and biochemical signals, etc.), in which cell assembly precedes other cell behaviors such as movement, replenishment, or differentiation . In particular, the arrangement of cells such as tenocytes, bone cells, and cardiomyocytes enables these living systems to effectively express specific cell functions, tissue repair, or regeneration. For this reason, efficient regulation of cellular assembly has become a central theme in biomedical research. [0003] To fully understand and control cellular assembly, much effort has been devoted to designing biological interfaces with control...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775C23C14/30C23C14/20B82Y40/00C40B40/02
Inventor 赵志远
Owner YANCHENG TEACHERS UNIV
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