Nursing and felt proofing finishing method for wool fabric with ultraviolet radiation based on keratin oligopeptides, polypeptides or keratin macro-molecules
A technology of keratin and macromolecules, applied in physical treatment, textile and paper making, peptide source, etc., can solve problems such as damage, complexity, size change, etc., and achieve the effect of simple process method, simple preparation method and mild conditions
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Embodiment 1
[0027] Preparation of keratin oligopeptides, polypeptides or macromolecular finishing solutions with wool fibers as raw materials, used for wool fabric care and anti-felting finishing methods through ultraviolet irradiation
[0028] a. Shred or grind the wool fibers, and immerse them in a pH 7 buffer solution containing 5g / L sulfite reducing agent according to the bath ratio of 1:10, and continuously stir at 80°C to pretreat the crushed fibers for 10 minutes Finally, add 20 U / g-fiber protease or disulfide bond isomerase to the system, use the enzyme-catalyzed method to stir and degrade the fiber at a constant temperature of 60°C for 0.5h, separate by centrifugation, take the supernatant, and obtain the molecular weight distribution by dialysis Uniform keratin oligopeptide, polypeptide or macromolecule solution;
[0029] b. To the above-mentioned keratin oligopeptide, polypeptide or macromolecule solution, according to crosslinking agent: keratin oligopeptide, polypeptide, macr...
Embodiment 2
[0033] Preparation of keratin oligopeptide, polypeptide or macromolecular finishing solution from wool fiber: UV irradiation for wool fabric care and anti-felting finishing method
[0034] a. Shred or grind the wool fibers, and immerse them in a pH 11 buffer solution containing 5M urea and 0.5M amino acid reducing agent according to the bath ratio of 1:50, vibrate and grind the fibers in water at 60°C for 5 hours, and then pass Centrifuge, take the supernatant, and obtain a keratin oligopeptide, polypeptide or macromolecule solution with uniform molecular weight distribution through dialysis;
[0035] b. Add protein heterologous cross-linking agent SDA to the above-mentioned keratin oligopeptide, polypeptide or macromolecule solution according to the cross-linking agent: keratin oligopeptide, polypeptide molar ratio of 100:1, and continuously stir the reaction at room temperature 1h, get reactive keratin oligopeptide, polypeptide or macromolecule finishing solution;
[0036] ...
Embodiment 3
[0039] Preparation of keratin oligopeptides, polypeptides or macromolecular finishing solutions from wool fibers: UV irradiation for sheep
[0040] Wool fabric care and anti-felting finishing method
[0041] a. Shred or grind the wool fibers, and immerse them in a buffer solution of pH 8 containing 20g / L guanidine reducing agent according to the bath ratio of 1:30, and continuously stir the pretreated pulverized fibers at room temperature for 60 minutes, and then pour them into Add 20 U / g-fiber protease or disulfide bond isomerase to the system, use enzyme-catalyzed method to stir and degrade the fiber at a constant temperature of 60°C for 3 hours, separate by centrifugation, and take the supernatant to obtain keratin-containing oligopeptides and polypeptides or macromolecule solution;
[0042] b. To the above-mentioned keratin oligopeptide, polypeptide or macromolecule solution, add protein heterologous cross-linking agent LC-SDA according to the molar ratio of cross-linking...
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