Cultivation method for increasing duckweed starch content and application

Abstract

The invention relates to the field of aquatic energy plant cultivation, in particular to a cultivation method for increasing the duckweed starch content and application. The cultivation method for increasing the duckweed starch content comprises the following steps that duckweed is inoculated on the surface of water, then, a uniconazole solution is sprayed to the surface of the duckweed growing inthe water, then, CO2 is introduced, and a target duckweed product is obtained. The method is not limited by the water, the duckweed starch content can be increased greatly no matter whether the duckweed is in relatively-eutrophic water or relatively-oligotrophic water, the duckweed with the high starch content can be cultivated in large scale in the polluted water, the high-content starch can berapidly accumulated, meanwhile, nitrogen and phosphorus in sewage can be effectively removed, on one hand, the production cost is lowered, and the duckweed with the high starch content is obtained; onthe other hand, the problem confronted at present that water is contaminated by nitrogen and phosphorus can be effectively relieved, and the method is easy to implement, low in cost and easy to applyand popularize.

Description

technical field [0001] The invention relates to the field of aquatic energy plant cultivation, in particular to a cultivation method and application for increasing duckweed starch content. Background technique [0002] With the increasing demand for energy, the scarcity of fossil energy and the seriousness of ecological problems, the development of renewable energy has received more and more attention. Bio-liquid fuel has become a global research hotspot due to its wide range of raw materials and renewability. Among them, fuel ethanol, as the only petroleum alternative energy suitable for existing engine systems, is the most widely used. At present, the main raw materials of fuel ethanol include terrestrial plants such as corn, sugarcane, cassava, and sweet potato, and the production cost is too high. In order to reduce the production cost, it is necessary to find cheap and sustainable plant materials as raw materials for production. The advantages of non-grain properties a...

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Problems solved by technology

At present, the methods of cultivating duckweed with high starch content are mainly: (1) under relatively oligotrophic conditions, that is, N and P concentrations are all lower than 0.1ppm to cultivate duckweed for 8 days (Xu J., Cui W., Cheng J.J. , Stomp A.M.Production of high-starch duckweed and its conversion tobioethanol.Biosystems Engineering.2011,110(2):67-72.), this method can make duckweed starch content up to 29.8%, but this method has the following deficiencies : ① high requirements on water bodies, this method requires duckweed to be cultivated in relatively oligotrophic water bodies, and with the increasing pollution of water bodies, it is difficult for natural water bodies to reach this standard, which greatly limits the application and promotion of this method; ② in relatively oligotrophic water bodies Cultivating duckweed in oligotrophic water, although the starch content of duckweed was increased, the growth of duckweed was severely inhibited, thereby reducing the biomass production of duckweed, resulting in no obvious increase in starch production; (2) in normal nutrition Under the conditions, that is, a certain concentration of uniconazole solution was sprayed in 1 / 6 concentration of Hoagland culture medium to cultivate duckweed for 10 days (LiuY., Fang Y., Huang M., et al. Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltiapunctata)II:transcriptome alterations of pathways involved in carbohydrate metabolism and endogenous hormone crosstalk.Biotechnology for Biofuels.2015,8:64), this method can make duckweed starch content up to 48.01%, the advantage of this method is that it is not limited by the water body , duckweed can be cultivated in water with normal nutrition, which can increase the starch content of duckweed without affecting the growth of duckweed, but the disadvantage of this method is that the starch content of duckweed is lower than 50%, which is not conducive to alcohol production during industrial fermentation. accumulation of concentration

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