Preparation method of decomposition agent for local straw returning to field

A decomposing agent and preparation method technology, applied in the direction of microbial-based methods, biochemical equipment and methods, applications, etc., can solve problems such as long cycle times

Inactive Publication Date: 2018-01-16
李琳
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cycle of using this bacterial agent to promote the complete decomposition of straw is too long,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0058] The preparation method of a kind of stalk returning field decomposing agent of the present invention is specifically implemented according to the following steps:

[0059] Step 1. Bacillus subtilis is cultured on a test tube strain slope, shake flask activation culture, seed tank fermentation and fermenter fermentation successively to obtain a Bacillus subtilis agent fermentation liquid, which is specifically implemented according to the following steps:

[0060] Step 1.1, inoculate Bacillus subtilis in LB slant medium A, control the temperature at 35°C to 37°C, and cultivate for 1 to 2 days, complete the slant culture of the test tube strain, and obtain the activated strain A;

[0061] The preparation method of LB slant medium A is as follows:

[0062] Add peptone, yeast extract, sodium chloride and agarose into water and mix well to get the product. The pH value is 7.2, and each liter of water contains 9g-11g peptone, 4g-6g yeast extract, and 9g-11g sodium chloride ,...

Embodiment 1

[0114] Bacillus subtilis was inoculated in LB slant medium A, the temperature was controlled at 35°C, and the culture time was 2 days to complete the slant culture of test tube strains to obtain activated strain A; wherein, the preparation method of LB slant medium A was as follows: Add peptone, yeast extract, sodium chloride and agarose to water, and mix well to obtain the pH value of 7.2. Each liter of water contains 9g of peptone, 4g of yeast extract, 9g of sodium chloride, and 14g of agarose; Inoculate the activated strain A into LB liquid medium A, and cultivate it at 35°C and 200rpm / min for 19h until the number of effective viable bacteria contained therein reaches 200 million / ml, complete the shake flask activation culture, and obtain the culture liquid A; Wherein, the preparation method of LB liquid culture medium A is: add peptone, yeast extract and sodium chloride to water, get final product after mixing evenly, its pH value is 7.2, and every liter of water contains 9...

Embodiment 2

[0120] Bacillus subtilis was inoculated in LB slant medium A, the temperature was controlled at 36°C, and the culture time was 1 day, and the slant culture of the test tube strain was completed to obtain the activated strain A; wherein, the preparation method of LB slant medium A was as follows: Add peptone, yeast extract, sodium chloride and agarose to water, and mix well to get it. The pH value is 7.2, and each liter of water contains 10g peptone, 5g yeast extract, 10g sodium chloride, and 15g agarose; Inoculate the activated strain A into LB liquid medium A, and cultivate it at 36°C and 180rpm / min for 18h until the number of effective viable bacteria contained therein reaches 150 million / ml, complete the shake flask activation culture, and obtain the culture liquid A; Wherein, the preparation method of LB liquid culture medium A is: add peptone, yeast extract and sodium chloride to water, get final product after mixing evenly, its pH value is 7.2, and every liter of water co...

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PUM

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Abstract

The invention discloses a preparation method of a decomposition agent for local straw returning to the field. The method comprises steps as follows: a bacillus subtilis agent fermentation broth is obtained from bacillus subtilis sequentially through slant culture of strains in a test tube, activating culture in a shake flask, fermentation in a seed tank and fermentation in a fermentation tank; anaspergillus niger agent fermentation broth is obtained from aspergillus niger sequentially through slant culture of strains in the test tube, activating culture in the shake flask, fermentation in theseed tank and fermentation in the fermentation tank; a trichoderma reesei agent fermentation broth is obtained from trichoderma reesei sequentially through slant culture of strains in the test tube,activating culture in the shake flask, fermentation in the seed tank and fermentation in the fermentation tank; a saccharomyces cerevisiae fermentation broth is obtained from saccharomyces cerevisiaesequentially through slant culture of strains in the test tube, activating culture in the shake flask, fermentation in the seed tank and fermentation in the fermentation tank; the bacillus subtilis agent fermentation broth, the aspergillus niger agent fermentation broth, the trichoderma reesei agent fermentation broth and the saccharomyces cerevisiae fermentation broth are mixed, and the decomposition agent for local straw returning to the field is obtained. The decomposition agent obtained with the preparation method has high decomposition efficiency and short decomposition time.

Description

technical field [0001] The invention belongs to the technical field of straw decomposing agents, and in particular relates to a preparation method of a decomposing agent for field return of straws in situ. Background technique [0002] Straw returning is a method of applying straw as a by-product of crops to the soil directly or after stacking and decomposing. Straw contains a large amount of fresh organic materials. After returning to the farmland, after a period of decomposition, it can be transformed into organic matter and available nutrients, which can improve soil physical and chemical properties and supply certain nutrients. Straw returning to the field can promote agricultural water saving, cost saving, increase in production and efficiency, and has received full attention in environmental protection and sustainable agricultural development. [0003] Returning straw to the field has many advantages. Straw returning to the field can increase the fresh organic matter...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N1/18C05G3/04C05F11/08C12R1/125C12R1/685C12R1/885C12R1/865C05G3/80
Inventor 李琳
Owner 李琳
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