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Gold nanocluster-based 6-mercaptopurine detection method and kit

A technology of gold nano-clusters and detection kits, which is applied in the field of analytical chemistry and nanometers, can solve the problems of complex measurement steps, long response time, and poor reproducibility, and achieve the effects of easy promotion and use, short detection time, and simple operation

Active Publication Date: 2018-01-16
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned methods have certain defects, such as the need for expensive instruments and toxic solvents, complicated determination steps, poor reproducibility, and long response time, etc.
In addition, some methods are severely interfered by biothiols (such as cysteine, homocysteine, glutathione, etc.)

Method used

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  • Gold nanocluster-based 6-mercaptopurine detection method and kit
  • Gold nanocluster-based 6-mercaptopurine detection method and kit
  • Gold nanocluster-based 6-mercaptopurine detection method and kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1 mL of 0.4 mol / L sodium hydroxide solution and 1.6 mL of 20 mg / mL chloroauric acid were mixed in advance, and then 7.4 mL of 50 mg / mL carboxylated chitosan solution and 10 mL of 0.1 mol / L dithiothreitol solution, shake well and place in a water bath at 37°C for a constant temperature reaction for 8 hours to obtain a carboxylated chitosan-dithiothreitol-gold nanocluster solution. The reaction solution changed from light yellow to colorless. The reacted gold nanocluster solution was dialyzed in double distilled water for 24 h with a dialysis bag with a molecular weight cut off of 3500 to obtain a purified carboxylated chitosan-dithiothreitol-gold nanocluster solution. The resulting carboxylated chitosan-dithiothreitol-gold nanoclusters are strongly fluorescent with a maximum emission wavelength of 650 nm (see figure 1 ).

Embodiment 2

[0040] Take 0.1 mL of the 1.6 mg / mL gold nanocluster solution prepared in Example 1 and add it to 1.9 mL of phosphate buffer (20 mmol / L, pH=7.4) containing 1 mmol / L 6-mercaptopurine, and mix well After reacting at room temperature for 10 min, the emitted light intensity value of the system at 650 nm was significantly lower than that of Example 1 (see figure 2 ).

Embodiment 3

[0042] Take 0.1 mL of the 1.6 mg / mL gold nanocluster solution prepared in Example 1 and add it to 1.9 mL of phosphate buffer (20 mmol / L, pH=7.4) containing 1 mmol / L 6-mercaptopurine, and mix well Then react at room temperature for 10 min. At the same time, a group of blank control groups without 6-mercaptopurine was set up. After the reaction was completed, the color change was observed under ultraviolet light. Such as image 3 As shown, the red fluorescence of gold nanoclusters was almost completely quenched after adding 6-mercaptopurine.

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Abstract

The invention discloses a gold nanocluster-based 6-mercaptopurine detection method and a kit. With use of specific interaction of carboxylated chitosan-dithiothreitol-gold nanoclusters and 6-mercaptopurine, fluorescence of the carboxylated chitosan-dithiothreitol-gold nanoclusters is quenched, and a carboxylated chitosan-dithiothreitol-gold nanocluster solution is added to a phosphate buffer solution containing 6-mercaptopurine; with increasing of the concentration of 6-mercaptopurine, the fluorescence intensity value F650 of the carboxylated chitosan-dithiothreitol-gold nanoclusters at 650 nmis decreased, so that the content of 6-mercaptopurine is determined. The invention provides a new detection method for 6-mercaptopurine, wherein the method has the advantages of high test sensitivity, good specificity, good accuracy, simple detection process, good stability, simple operation, short detection time, high sensitivity, strong specificity and the like.

Description

technical field [0001] The invention relates to a rapid and ultra-sensitive content detection method for 6-mercaptopurine, in particular to a fluorescence detection method for 6-mercaptopurine based on carboxylated chitosan-dithiothreitol-gold nanoclusters and its reagents Box, belonging to the field of analytical chemistry and nanotechnology. Background technique [0002] As we all know, 6-mercaptopurine is a similar compound of adenine, which is widely used in the treatment of acute leukemia, inflammatory bowel disease, epithelial carcinoma, choriocarcinoma, polycysticosis and psoriasis, etc. Although 6-mercaptopurine has been proven to be an effective drug, it can cause some serious side effects, including bone marrow suppression and gastrointestinal depression. In addition, the concentration of 6-mercaptopurine in the blood varies greatly with individuals and the safety range is very narrow, and an increase in the concentration of a small dose will produce obvious toxic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 陈伟黄开源邓豪华刘银环彭花萍
Owner FUJIAN MEDICAL UNIV
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