Application of Bacillus megaterium YJB3 in plant growth promotion and bio-control

A Bacillus megaterium, plant growth-promoting technology, applied in plant growth regulators, plant growth regulators, botanical equipment and methods, etc., can solve problems such as soil ecological environment damage, agricultural product quality decline, soil environmental pollution, etc., to achieve Effects of reducing agricultural non-point source pollution, improving nutrient levels, and promoting plant growth

Active Publication Date: 2018-01-19
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Due to the excessive pursuit of agricultural production, the abuse of agricultural chemicals such as pesticides, fertilizers and plastic film, the soil ecological environment has been severely damaged, the soil environment has been seriously polluted by organic pollutants and heavy metals, and the soil fertility has seriously declined, resulting in a decline in the quality of agricultural products.
Residents generally feel that the quality of the current agricultural products has declined, and even the pollution of agricultural products has seriously damaged human health.

Method used

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  • Application of Bacillus megaterium YJB3 in plant growth promotion and bio-control
  • Application of Bacillus megaterium YJB3 in plant growth promotion and bio-control
  • Application of Bacillus megaterium YJB3 in plant growth promotion and bio-control

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The quantitative determination method of strain B. megaterium YJB3 phosphate-solubilizing ability refers to Liu et al. and their effects on plant growth and phosphorus mobilization in tropical soils. Biol. Fertile. Soils 50, 927-937.).

[0031] Ammonium molybdate hydrochloride solution: weigh 20g (NH 4 ) 6 MoO 24 4H 2 O was dissolved in 200mL of deionized water, stirred evenly and added to 425mL of concentrated hydrochloric acid, and then added to 50mL of deionized water after mixing.

[0032] Reducing agent: weigh 15g NaHSO 3 Dissolve in 250mL deionized water, mix well and add 1.5g Na 2 SO 4 and 0.5g of 1,2,4-aminonaphtholsulfonic acid, then dilute to 500mL, and store at 4°C in the dark for later use.

[0033] Phosphorus standard solution: weigh 0.10975g recrystallized KH 2 PO 4Dissolve in 0.1M HCl solution, and dilute to 500mL. The solution is 50 μg / mL phosphorus standard solution.

[0034] Drawing of phosphorus standard curve: draw phosphorus standard solu...

Embodiment 2

[0036] The strain B. megaterium YJB3 was inoculated on Ashby solid medium (nitrogen-free) (Kryuchkova, Y.V., Burygin, G.L., Gogoleva, et al., 2014. Isolation and characterization of aglyphosate-degrading rhizosphere strain, Enterobacter cloacae K7. Microbiol. Res. 169,99-105.), cultivated at 30°C for 3 days, observed its growth, and it was positive if there were colonies growing.

[0037] Nitrogenase activity was determined by acetylene reduction method in nmol C 2 h 4 / (h·mL) represents the activity of nitrogenase.

[0038]

[0039] Determination of nitrogenase activity of the strain YJB3 105.56 ~ 135.89nmol C 2 h 4 / (h·mL).

Embodiment 3

[0041] MM9 salt solution (g L -1 ): Na 2 HPO 4 60,KH 2 PO 4 3, NaCl 5, NH 4 Cl 10;

[0042] MKB (King's Medium L -1 ): K 2 HPO 4 2.5g, MgSO 4 ·7H 2 O 2.5g, acid hydrolyzed casein 5g, glycerin 13mL, pH 7.2.

[0043] CAS (chrome azure S) dye medium: take 2.7mg FeCl 3 ·6H 2 O was dissolved in 10mL of 10mM hydrochloric acid and added to 50mL of 1.21mg·mL -1 chrome azure solution, and then slowly pour this solution into 40mL1.82mg·mL -1 In the CTAB (cetyltrimethylammonium bromide) solution, stir continuously to avoid bubbles, forming a dark blue solution, and autoclave at 121°C for 20min. Add 30.24g Pipes (piperazine diethanolsulfonic acid) and 100mL MM9 salt solution to 750mL deionized water, adjust the pH of the solution to 6.8 with 50% NaOH, 15g agar, and autoclave at 121°C for 20min.

[0044] Quantitative determination of siderophilin production: inoculate the strain B. megaterium YJB3 in MKB liquid medium, culture at 30°C, 150rpm shaker for 3 days, centrifuge...

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Abstract

The invention discloses an application of Bacillus megaterium YJB3 in plant growth promotion and bio-control, and belongs to the field of plant protection. The Bacillus megaterium YJB3 has phosphorusdissolving, nitrogen fixing, ferritin producing and other plant growth promoting functions, and also has a plant pathogen inhibiting bio-control effect. Endophytes are colonized in plants or rhizospheres in a rhizosphere inoculation, blade spray or smear treatment or seed or root soaking manner by using a plant endophyte YJB3 bacterium liquid, so the nutrient level of inoculated plants is improved, the disease resistance is improved, the plant biomass is increased, and the plant growth is promoted. The Bacillus megaterium YJB3 is simple, safe and economic to operate, can reduce the use amountsof pesticides and chemical fertilizers in agricultural production and reduce agricultural non-point source pollution, and is of great significance to improving the ecological environment of farmlandsoil and improving the quality of agricultural products.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to the application of Bacillus megaterium YJB3 in promoting plant growth and biocontrol. Background technique [0002] Due to the excessive pursuit of agricultural production and the abuse of agricultural chemicals such as pesticides, fertilizers, and mulch films, the soil ecological environment has been severely damaged. The soil environment has been seriously polluted by organic pollutants and heavy metals, and soil fertility has declined severely, resulting in a decline in the quality of agricultural products. Residents generally feel that the quality of the current agricultural products has declined, and even the pollution of agricultural products has seriously damaged human health. Studies have found that plant endophytes are systematically distributed in various organs, cells and intercellular spaces of plants. After colonizing the rhizosphere or root surface, cert...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P21/00A01P1/00A01P3/00
Inventor 莫测辉冯乃宪李彦文喻娇赵海明蔡全英李慧左娜
Owner JINAN UNIVERSITY
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