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Preparation method of dendrobium officinale fungus fermented product, fermented product and application of fermented product

A fermented product, the technology of Dendrobium candidum, applied in the field of bioengineering, can solve the problems of poor solubility, easy layering, and rough taste of Dendrobium powder, and achieve the effects of improving immunity, mellow taste, and excellent taste

Pending Publication Date: 2018-01-26
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE +2
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  • Abstract
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  • Claims
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Problems solved by technology

[0012] The patent with the application number 201610422473 discloses a highly soluble probiotic fermented dendrobium powder and its preparation and application. It uses cellulase to enzymatically treat dendrobium before fermentation, which improves the solubility and palatability of dendrobium, and at the same time the fiber The sugars produced by enzymatic hydrolysis can be used as the substrate of probiotic fermentation, which solves the shortcomings of the existing probiotic fermented dendrobium powder, such as poor solubility, easy layering after brewing, and rough taste

Method used

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  • Preparation method of dendrobium officinale fungus fermented product, fermented product and application of fermented product
  • Preparation method of dendrobium officinale fungus fermented product, fermented product and application of fermented product

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preparation example Construction

[0030] The preparation method of the dendrobium candidum fungal fermentation product of the present invention comprises the steps of preparation of dendrobium, strain activation, liquid seed cultivation, dendrobium fungus fermentation, and treatment of fermented products, and the specific steps are as follows:

[0031] A, preparation of dendrobium: remove the inedible parts of the fresh strips of Dendrobium candidum and wash them, cut into small sections after natural drying, beating to obtain dendrobium pulp for subsequent use;

[0032] B. Activation of strains: transfer the preserved fungal strains of medicinal and edible homology to solid culture media on inclined planes and cultivate them at 28-30°C for 5-10 days;

[0033] C. Liquid seed culture: cut a piece of the above-mentioned activated medicinal and edible homologous fungal strain and inoculate it into a 300mL triangular flask filled with 100mL liquid medium, and culture it with shaking at 140-150r / min at 28-30°C for 7 ...

Embodiment 1

[0055] S100: Remove the inedible parts of the fresh Dendrobium candidum strips, wash them, dry them naturally, cut them into sections, and then make a pulp according to the mass-volume ratio of 1:5 (g / mL) of feed water, and store the dendrobium pulp at -20°C for refrigeration spare.

[0056] S200: The preserved medicinal and edible fungus species Grifola frondosa G 2 m 5 10 strains (preservation number GIM5.10) were cut with an inoculation shovel from the inclined test tube of the parent species to take 1 cm 2 Mycelium pieces of large and small sizes are inoculated at the upper 1 / 3 of the bottom of the slope of the PDA slant medium, and cultured at 28°C for 5-10 days until the mycelia cover the slope; the composition of the PDA slant medium includes by mass percentage: Potato 20%, glucose 2%, agar 2%, chloramphenicol 0.01% and the rest of water.

[0057] S300: Cut off the medicinal and edible homologous fungal strains activated by S200 with an inoculation spatula to take 1c...

Embodiment 2

[0061] S100: Remove the inedible parts of the fresh Dendrobium candidum strips, wash them, dry them naturally, cut them into sections, and then make a pulp according to the mass-volume ratio of feed water 1:10 (g / mL), and store the dendrobium pulp at -20°C for refrigeration spare.

[0062] S200: Preserved medicinal and edible homologous fungal strain Poria cocos G 2 m 5 219 strains (preservation number GIM5.219) were cut with an inoculation spatula to take 1 cm from the inclined tube of the parent species. 2 Mycelium pieces of large and small sizes are inoculated at the upper 1 / 3 of the bottom of the slope of the PDA slant medium, and cultured at 28°C for 5-10 days until the mycelia cover the slope; the composition of the PDA slant medium includes by mass percentage: Potato 20%, glucose 2%, agar 1.5%, chloramphenicol 0.01% and the rest of water.

[0063] S300: Cut off the medicinal and edible homologous fungal strains activated by S200 with an inoculation spatula to take 1c...

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Abstract

The invention discloses a preparation method of a dendrobium officinale fungus fermented product, the fermented product and an application of the fermented product. The preparation method of the dendrobium officinale fungus fermented product includes the steps of dendrobium officinale preparation, strain activation, liquid seed culture, dendrobium officinale fungus fermentation and fermented product processing. The fermented product is obtained by the preparation method of the dendrobium officinale fungus fermented product. The application comprises that the dendrobium officinale fungus fermented product is used for preparing food, health food, cosmetics and / or drugs. Dendrobium officinale is fermented with medicinal and edible fungi containing cellulose and other substances, dendrobium cellulose and the like are decomposed into small-molecular carbon sources, and the beneficial ingredients of the dendrobium officinale are retained; and moreover, the fermented product has mellow mouthfeel, has both faint scent of the dendrobium officinale and unique flavor of the fungi, also is rich in fungal polysaccharides, saponins, polyphenols, flavonoids and other active substances; the effective ingredients are easily absorbed in intestines, and has the efficacies of improving immunity, resisting tumor, resisting oxidation, reducing blood fat and blood pressure, regulating the balance ofintestinal microflora and the like.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and specifically relates to a preparation method of a dendrobium officinale fungus fermented product with the functions of improving immunity, anti-tumor, anti-oxidation, lowering blood fat and blood pressure, and regulating the balance of intestinal flora, as well as the fermented product and its application. Background technique [0002] Macrofungi is an ancient organism. There are about 10,000 to 20,000 species of macrofungi in my country, and about 8,000 species have been described. Both "Shen Nong's Materia Medica" and "Compendium of Materia Medica" have records of the medicinal value of fungi. The macrofungi with the same source of medicine and food refers to a type of fungus that will not cause human poisoning after eating, and can form fleshy or gelatinous fruiting bodies that are visible to the naked eye. The fungi with the same source of medicine and food are rich in terpenoids (...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23L31/00A23L33/10A61K36/8984A61K8/9794A61P37/04A61P35/00A61P39/06A61P3/06A61P9/12A61P1/00C12R1/645
Inventor 赵声兰李雪刘竞元陈朝银杨映宁
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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