Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for high-flux directed mutagenesis of glyphosate resistance of sugarcane through plasma

A plasma and anti-glyphosate technology, applied in horticultural methods, botanical equipment and methods, angiosperms/flowering plants, etc., can solve the inconvenient operation of test tube slant medium, obvious differences in mutagenic effects, and small processing capacity and other problems, to achieve the effect of obvious difference in mutagenic effect, intuitive judgment, and less pollution

Pending Publication Date: 2018-02-02
GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above existing problems, the present invention provides a high-throughput method for mutagenizing sugarcane embryogenic callus for the first time, which eliminates the problem of carcinogenicity caused by chemical mutagens to humans, allows technicians to work with peace of mind, and not only solves the traditional The problem that sugarcane callus is easy to pollute can also meet the needs of large-scale mutagenesis of sugarcane callus, and solve the problem of inconvenient operation and small processing volume of test tube slant medium. The survival rate of callus can quickly determine the time of mutagenesis treatment, and through directional stress screening, the efficiency of mutagenesis breeding can be improved, and the mutagenesis rate can reach 2.50%-2.77%

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for high-flux directed mutagenesis of glyphosate resistance of sugarcane through plasma
  • Method for high-flux directed mutagenesis of glyphosate resistance of sugarcane through plasma
  • Method for high-flux directed mutagenesis of glyphosate resistance of sugarcane through plasma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] A fine sugarcane cultivar callus initiation

[0065] 1) Preparation of inoculation materials

[0066] After the cane stem grows, cut off the tail tip of the strong cane plant, peel off the outer leaves, disinfect with 75% alcohol for 30 seconds under sterile conditions, cut off the outer layer and both ends of the leaves (leaf sheath), and leave 10mm-50mm above the growth point Young leaves (tender sheaths) are cut into thin slices of about 2 mm, and 1 / 2 ring cut for inoculation.

[0067] 2) Obtaining sugarcane embryogenic callus

[0068] Inoculate the prepared slices in medium CM1, culture them in the dark at 26°C-28°C, 6-8 pieces per dish, induce callus, and then in medium CM1 (MS+2,4-D 2.0 μmol·L -1 + sucrose 30g·L -1 +Agar 8g·L -1 , pH value 6.2) subcultured 1-2 times, 6-8 pieces per dish, to make it produce embryogenic callus.

[0069] B high-throughput arrangement

[0070] The sugarcane embryogenic callus subcultured 2-3 times was clamped into small pieces ...

Embodiment 2

[0087] On July 15, 2015, the HPD-280 plasma machine was used to mutate the embryogenic callus of Yuetang 93-159 sugarcane. The mutagenesis power was set to 140W, and the irradiation mutagenesis time was 80s, 100s, and 120s, respectively. , 140s, 160s, 180s. Other operations in embodiment 2 are all the same as embodiment 1. The survival rate and mutation rate of embryogenic callus in each group were detected.

[0088] The test results are shown in Table 1. Among them, the survival rate of the embryogenic callus treated at 120s was the highest, 18.3%, which was significantly higher than that of other treatments, and it could differentiate into adult plants after two rounds of glyphosate selection, with a mutagenesis rate of 2.50%. .

[0089] Table 1 Plasma mutagenesis of Yuetang 93-159 sugarcane embryogenic callus under the conditions of power 140W and treatment time 80-180s

[0090]

[0091]

[0092] Note: Different lowercase English letters after the data in the same ...

Embodiment 3

[0096] On May 4, 2016, the HPD-280 plasma machine was used to mutate the embryogenic callus of Yuetang 93-159 sugarcane, and the irradiation time was 120s. The mutagenic power was set to 140W, 170W, and 200W, respectively. Other operations in implementation 3 are the same as implementation 1. The survival rate and mutation rate of embryogenic callus in each group were detected.

[0097] The test results are shown in Table 2. Among them, the survival rate of embryogenic callus treated with power 140W was the highest, which was 62.4%. It is 140W, reaching 2.77%.

[0098] Table 2 Plasma mutagenesis of Yuetang 93-159 sugarcane embryogenic callus under the conditions of power 140-200W and treatment time 120s

[0099]

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for high-flux directed mutagenesis of the glyphosate resistance of sugarcane through plasma. The method comprises the following steps: carrying out irradiation mutagenesis treatment on embryonic calluses of the sugarcane under aseptic conditions by a plasma instrument under a mutagenesis power being 140-200 W and a discharging spacing being 35-45 mm for 110-140 s under the protection of nitrogen; and carrying out buffering culture, medium-high-concentration glyphosate stress screening and differentiation seedling formation on the processed calluses, and carrying out bottled seedling glyphosate stress screening and potted seedling glyphosate foliar application stress screening. The method has the advantages of safety in operation, simplicity, easiness in implementation, large throughout, less pollution, and realization of high screening efficiency, reduction of the subsequent screening workload and visual determination of the resistance of mutant strainsdue to the implementation of oriented stress screening.

Description

technical field [0001] The invention relates to a method for plasma high-flux directed mutagenesis of sugarcane resistance to glyphosate. Background technique [0002] Sugarcane (Sugarcane) is an important sugar crop in my country, and sugarcane sugar accounts for more than 90% of my country's sugar production. The development of the sugar industry is inseparable from the improvement of sugarcane varieties. The contribution rate of fine varieties to the technological progress of cane sugar production reaches 60%. It is the main core technology for the sustainable development of my country's cane sugar industry and the improvement of the international competitiveness of the sugar industry. Excellent varieties have more or less shortcomings in the production process, such as poor drought resistance or glyphosate resistance. Sugarcane mutation breeding is a fast way to improve cultivated sugarcane varieties, and it is to alleviate the situation of single sugarcane variety stru...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H1/06A01H1/04
CPCA01H1/06A01H4/008A01H4/002A01H6/46A01H1/1235
Inventor 黄忠兴徐苑娴何慧怡樊丽娜劳方业凌秋平
Owner GUANGDONG PROVINCIAL BIOENGINEERING INST (GUANGZHOU SUGARCANE IND RES INST)
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com