Method for culturing edible-medicinal fungi by means of liquid static culture
A technology for edible and medicinal fungi and liquid culture, which is applied in sterilization methods, microorganism-based methods, and methods of supporting/immobilizing microorganisms, etc. Activity enhancement and other issues, to achieve the effect of improving biological activity and nutritional value, expanding culture area, and fast growth
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Embodiment 1
[0062] Bacteria: Ganoderma lucidum
[0063] Liquid seed medium (g L -1 ): Potato 200, Glucose 20, Natural pH.
[0064] Liquid stationary medium (g L -1 ): glucose 20, tryptone 5, YNB (amino-free yeast) 5, KH 2 PO 4 4.5, MgSO 4 ﹒ 7H 2 O2, pH 6.0.
[0065] Specifications of sterile culture container: The incubator unit is a cylinder with a radius of 50mm and a height of 60mm. The bacteria intercepting device is located inside the container at a distance of 25mm from the bottom of the container.
[0066] The strains preserved on the slant of Ganoderma lucidum were inserted into a 250mL sterile Erlenmeyer flask containing 80mL of liquid seed medium, and cultured at 30°C and 150rpm for 7 days to obtain the seed liquid, which was then transferred to a new 250mL sterile flask with a rotor and glass beads. The triangular flask was broken to obtain a homogenized seed solution. Add 230mL of liquid static culture medium into the culture container unit, inoculate 5.75mL of liquid...
Embodiment 2
[0076] Strain: Cordyceps sinensis
[0077] Liquid seed medium (g L -1 ): potato 200, glucose 20, pH natural.
[0078] Liquid stationary medium (g L -1 ): Sucrose 20, Peptone 20, KH 2 PO 4 1. MgSO 4 ·7H 2 O 0.5, pH natural.
[0079] Specifications of sterile culture container: The incubator unit is a cylinder with a radius of 50mm and a height of 60mm. The bacteria intercepting device is located inside the container at a distance of 25mm from the bottom of the container.
[0080] Cordyceps slant-preserved strains were inserted into a 250mL sterile Erlenmeyer flask containing 80mL liquid seed medium, and cultured at 25°C and 150rpm for 5 days, which was the seed liquid, which was transferred to a new 250mL sterile flask with a rotor and glass beads. The triangular flask was broken to obtain a homogenized seed solution. Add 230mL of liquid static culture medium to a sterile circular culture container, inoculate 5.75mL of liquid homogenized seed solution, place it in a con...
Embodiment 3
[0090] Bacteria: Ganoderma lucidum
[0091] Liquid seed medium (g L -1 ): Potato 200, Glucose 20, Natural pH.
[0092] Liquid stationary medium (g L -1 ): glucose 20, tryptone 5, YNB (amino-free yeast) 5, KH 2 PO 4 4.5, MgSO 4 ﹒ 7H 2 O2, pH 6.0.
[0093] Sterile culture container specifications: The incubator unit is a rectangular parallelepiped, detachable, with a length and width of 200mm and a height of 100mm. The bacteria intercepting device is located inside the container at a distance of 45mm from the bottom of the container.
[0094] The strains preserved on the slant of Ganoderma lucidum were inserted into a 250mL sterile Erlenmeyer flask containing 80mL of liquid seed medium, and cultured at 30°C and 150rpm for 7 days to obtain the seed liquid, which was then transferred to a new 250mL sterile flask with a rotor and glass beads. The triangular flask was broken to obtain a homogenized seed solution. Add 2L of liquid static medium into the culture container uni...
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